University of Tehran Iranian Journal of Plant Protection Science 2008-4781 55 2 2025 02 19 Evaluation of the combination of Bacillus velezensis JPS19 and Bacillus thuringiensis E13 in the control of Fusarium oxysporum f.sp. radicis-lycopersici in tomato Evaluation of the combination of Bacillus velezensis JPS19 and Bacillus thuringiensis E13 in the control of Fusarium oxysporum f.sp. radicis-lycopersici in tomato 349 366 103947 10.22059/ijpps.2025.397451.1007082 FA Sara Salari Ganjabad Department of Phytomedicine, Faculty of Agriculture, University of Tehran, Karaj, Iran Keivan Behboudi Department of Phytomedicine, Faculty of Agriculture, University of Tehran, Karaj, Iran. Journal Article 2025 06 28 Tomato root and crown rot, caused by the fungus Fusarium oxysporum f. sp. radicis-lycopersici (UJ152) (FORL), is a significant soil-borne disease and a major factor causing damage to tomato production worldwide. The use of soil-borne antagonists to enhance yield and crop protection is a promising approach in modern sustainable agricultural systems.The controlling effects of a bacterial combination on the mycelial growth of the pathogen were investigated by culture in the laboratory. Two strains, Bacillus thuringiensis E13 and B. velezensis JPS19, were able to form an inhibition halo against the mycelial growth of the FORL (UJ152) pathogen.In this study, various treatments, including the inoculation of the two bacterial strains (B. thuringiensis E13 and B. velezensis JPS19), inoculation with the pathogenic fungus, and the combination of both bacterial strains, were evaluated under greenhouse conditions to investigate the reduction in disease severity and inhibition of the causal fungus. While 73.33% disease severity was observed in the inoculated control sample, the application of the two bacterial strains reduced the disease severity to 6.66%.A suspension of the combination of the two aforementioned bacterial strains was prepared at 109 concentration, and the inhibition of pathogen growth was investigated under greenhouse conditions. It was observed that the combination of the two mentioned strains resulted in an 8.83% improvement in tomato plant growth compared to the healthy control, and also led to an increase in the fresh weight of the root and shoot of the plants treated with the two bacterial strains compared to the plants inoculated with FORL (UJ152). Tomato root and crown rot, caused by the fungus Fusarium oxysporum f. sp. radicis-lycopersici (UJ152) (FORL), is a significant soil-borne disease and a major factor causing damage to tomato production worldwide. The use of soil-borne antagonists to enhance yield and crop protection is a promising approach in modern sustainable agricultural systems.The controlling effects of a bacterial combination on the mycelial growth of the pathogen were investigated by culture in the laboratory. Two strains, Bacillus thuringiensis E13 and B. velezensis JPS19, were able to form an inhibition halo against the mycelial growth of the FORL (UJ152) pathogen.In this study, various treatments, including the inoculation of the two bacterial strains (B. thuringiensis E13 and B. velezensis JPS19), inoculation with the pathogenic fungus, and the combination of both bacterial strains, were evaluated under greenhouse conditions to investigate the reduction in disease severity and inhibition of the causal fungus. While 73.33% disease severity was observed in the inoculated control sample, the application of the two bacterial strains reduced the disease severity to 6.66%.A suspension of the combination of the two aforementioned bacterial strains was prepared at 109 concentration, and the inhibition of pathogen growth was investigated under greenhouse conditions. It was observed that the combination of the two mentioned strains resulted in an 8.83% improvement in tomato plant growth compared to the healthy control, and also led to an increase in the fresh weight of the root and shoot of the plants treated with the two bacterial strains compared to the plants inoculated with FORL (UJ152).

https://ijpps.ut.ac.ir/article_103947_52f2d62f19fa475b4cdf9e76369543cc.pdf