University of Tehran Iranian Journal of Plant Protection Science 2008-4781 44 1 2013 03 21 Repellency Effect of Three Herbal Essential Oils on the Two--spotted Spider Mite, Tetranychus urticae Repellency Effect of Three Herbal Essential Oils on the Two--spotted Spider Mite, Tetranychus urticae 103 112 35627 10.22059/ijpps.2013.35627 FA Hamid Reza Sarraf Muayeri Assistant Professor, College of Agriculture, University of Zanjan Fatemeh Pirayeshfar Assistant Professor, College of Agriculture, University of Zanjan Orang Kavousi Assistant Professor, College of Agriculture, University of Zanjan Journal Article 2012 02 13 Two-spotted spider mite (<em>Tetranychus urticae</em> Koch), is one of the most seriously important  polyphagous pests that causes considerable damage to agricultural crops. In recent years, due to problems associated with the use of synthetic pesticides, herbal essential oils have received due attention as natural plant pest control compounds. In the present study, repellency effect of herbal plant essential oils extracted from some Lamiaceae family members namely: rosemary (<em>Rosmarinus officinalis</em> L.), savory (<em>Satureja hortensis</em> L.), and peppermint (<em>Mentha</em> <em>piperita</em> L (.were studied on <em>T. urticae</em> employing Twin Towers Bioassay (TTB) method. The experiments were conducted at 23±2°C, 60±5% relative humidity and  fully  light  photoperiodism. Bioassay experiments were carried out at 0.043, 0.073, 0.326 and 0.348 µL/cm² doses of the essential oils within four experimental intervals of time. The results indicated that peppermint essential oil had the most  repellent effect (91%) on female adults of <em>T. urticae</em> at highest dose (0.348 µL/cm²), but there was no significant difference observed between peppermint oil and the oils extracted from other herbal plant. (P> 0.05). In addition, the most persistency was observed for savory essential oil. Throughout the study, a new method was also introduced to evaluate the repellency effect of essential oils on some such herbivorous arthropods as mites.   Two-spotted spider mite (<em>Tetranychus urticae</em> Koch), is one of the most seriously important  polyphagous pests that causes considerable damage to agricultural crops. In recent years, due to problems associated with the use of synthetic pesticides, herbal essential oils have received due attention as natural plant pest control compounds. In the present study, repellency effect of herbal plant essential oils extracted from some Lamiaceae family members namely: rosemary (<em>Rosmarinus officinalis</em> L.), savory (<em>Satureja hortensis</em> L.), and peppermint (<em>Mentha</em> <em>piperita</em> L (.were studied on <em>T. urticae</em> employing Twin Towers Bioassay (TTB) method. The experiments were conducted at 23±2°C, 60±5% relative humidity and  fully  light  photoperiodism. Bioassay experiments were carried out at 0.043, 0.073, 0.326 and 0.348 µL/cm² doses of the essential oils within four experimental intervals of time. The results indicated that peppermint essential oil had the most  repellent effect (91%) on female adults of <em>T. urticae</em> at highest dose (0.348 µL/cm²), but there was no significant difference observed between peppermint oil and the oils extracted from other herbal plant. (P> 0.05). In addition, the most persistency was observed for savory essential oil. Throughout the study, a new method was also introduced to evaluate the repellency effect of essential oils on some such herbivorous arthropods as mites.   https://ijpps.ut.ac.ir/article_35627_6aad2e73abed33e0e504e3ea0f3bd218.pdf