University of Tehran Iranian Journal of Plant Protection Science 2008-4781 44 2 2014 02 20 Effect of Culture Substrates on Virulence of Metarhizium anisopliae Conidia and Blastospores against Sunn Pest, Eurygaster integriceps Effect of Culture Substrates on Virulence of Metarhizium anisopliae Conidia and Blastospores against Sunn Pest, Eurygaster integriceps 225 234 36668 10.22059/ijpps.2014.36668 FA Siyamak Roshandel PhD Student, College of Agriculture andNatural Resources,University of Tehran, Tehran, Iran Reza Talaei-Hassanlouei Associate Professor,College of Agriculture andNatural Resources,University of Tehran, Tehran, Iran Hasan Askary Associate Professor, Research InstituteofPlant Protection,Tehran, Iran Hossein Allahyari Associate Professor,College of Agriculture andNatural Resources,University of Tehran, Tehran, Iran Rasoul Marzban Associate Professor, Research InstituteofPlant Protection,Tehran, Iran Journal Article 2013 10 01 Virulence of blastospores and conidia of the fungus Metarhizium anisopliae were investigated<br />on different instar nymphs and adults of Sunn pest, Eurygater integriceps. Experiments were<br />carried out through a factorial arrangement in a completely randomized design of three<br />replications. The bioassays were performed through immersion method. Results revealed<br />significant differences among virulence of blastospores and of conidia produced in different<br />media.The highest vs the lowest total mortality percentages of the second instar nymphs were<br />recorded as 100 and 41.6% on rice bran vs wheat bran+ rice bran media respectively in<br />blastopore treatments. As for conidia, the highest total mortality percentage of second instar<br />nymphs was 100% on wheat bran, Behnoush waste barley and rice flour media whilst the<br />lowest total mortality percentage recorded as 86.1% on maize medium. Minimum LT50 was<br />recorded (2.3 days) on wheat bran + yeast extract medium in the second instar nymphs whilst<br />the highest LT50 recorded (10.1 days) on wheat bran+ rice bran extract medium for the fourth<br />instar nymphs in blastopore treatments. Minimum LT50 was recorded (4.7 days) on the rice<br />bran medium and at second instar nymphs while the highest recorded (13.1 days) for the fourth<br />instar nymphs by conidia harvested from non-broken rice medium. Virulence of blastospores and conidia of the fungus Metarhizium anisopliae were investigated<br />on different instar nymphs and adults of Sunn pest, Eurygater integriceps. Experiments were<br />carried out through a factorial arrangement in a completely randomized design of three<br />replications. The bioassays were performed through immersion method. Results revealed<br />significant differences among virulence of blastospores and of conidia produced in different<br />media.The highest vs the lowest total mortality percentages of the second instar nymphs were<br />recorded as 100 and 41.6% on rice bran vs wheat bran+ rice bran media respectively in<br />blastopore treatments. As for conidia, the highest total mortality percentage of second instar<br />nymphs was 100% on wheat bran, Behnoush waste barley and rice flour media whilst the<br />lowest total mortality percentage recorded as 86.1% on maize medium. Minimum LT50 was<br />recorded (2.3 days) on wheat bran + yeast extract medium in the second instar nymphs whilst<br />the highest LT50 recorded (10.1 days) on wheat bran+ rice bran extract medium for the fourth<br />instar nymphs in blastopore treatments. Minimum LT50 was recorded (4.7 days) on the rice<br />bran medium and at second instar nymphs while the highest recorded (13.1 days) for the fourth<br />instar nymphs by conidia harvested from non-broken rice medium. https://ijpps.ut.ac.ir/article_36668_fe572f2d006b2e36644bfec3e5d94f51.pdf