University of Tehran Iranian Journal of Plant Protection Science 2008-4781 46 1 2015 03 21 Study on genetic structure of Pyrenophora graminea populations the causal agent of barley leaf stripe disease using ISSR marker Study on genetic structure of Pyrenophora graminea populations the causal agent of barley leaf stripe disease using ISSR marker 161 177 54731 10.22059/ijpps.2015.54731 FA Khoshnood Nooralahi Assistant Professor, Department of Plant Protection, Plant Pathology, Faculty of Agriculture, University of Ilam Masoumeh Shahbazi Former Graduate Student, Plant Pathology, Faculty of Agriculture, University of Ilam Journal Article 2014 10 30 Barley leaf stripe caused by Pyrenophora graminea is one of the most important diseases of barley in Ilam province. In order to determine the genetic structure of P. graminea populations, sampling was randomly carried out on aerial parts of barley plants with leaf brown lesions, from different regions in Ilam province. A total of 69 P. graminea isolates were obtained from infected samples using potato dextrose agar media. Pathogenicity test and inoculation of seeds were done with Sandwich method, because causal agent cannot produce enough spores on artificial media. A set of eight Inter simple sequence repeat primers (ISSR) were used to determine genetic diversity of P. graminea isolates. This set ISSR primers revealed a total of 171 alleles for P. graminea isolates in all populations. The number of alleles varied from 16 to 30 for each marker. Cluster analysis were carried out with according Dice similarity coefficient and UPGMA method. Based on dendrogram thirteen groups were revealed. A Comparison of the parameters of genetic diversity in seven population showed that Darehshahr population has the highest genetic diversity (h= 0.32) and the smallest genetic distance was observed between population Asemanabad and Sirvan (0.019). The phonogram revealed two distinct groups, one group contained Darehshahr and the other Ivan, Asemanabad, Sirvan and Chardavol populations. Molecular analysis of variance to compare the genetic diversity within and between populations showed the greatest diversity related within populations (94%) and the level of genetic variation among populations was very low (6%). High level of genetic variation within populations can be justified by highest amount of gen flow (Nm= 4.576). Barley leaf stripe caused by Pyrenophora graminea is one of the most important diseases of barley in Ilam province. In order to determine the genetic structure of P. graminea populations, sampling was randomly carried out on aerial parts of barley plants with leaf brown lesions, from different regions in Ilam province. A total of 69 P. graminea isolates were obtained from infected samples using potato dextrose agar media. Pathogenicity test and inoculation of seeds were done with Sandwich method, because causal agent cannot produce enough spores on artificial media. A set of eight Inter simple sequence repeat primers (ISSR) were used to determine genetic diversity of P. graminea isolates. This set ISSR primers revealed a total of 171 alleles for P. graminea isolates in all populations. The number of alleles varied from 16 to 30 for each marker. Cluster analysis were carried out with according Dice similarity coefficient and UPGMA method. Based on dendrogram thirteen groups were revealed. A Comparison of the parameters of genetic diversity in seven population showed that Darehshahr population has the highest genetic diversity (h= 0.32) and the smallest genetic distance was observed between population Asemanabad and Sirvan (0.019). The phonogram revealed two distinct groups, one group contained Darehshahr and the other Ivan, Asemanabad, Sirvan and Chardavol populations. Molecular analysis of variance to compare the genetic diversity within and between populations showed the greatest diversity related within populations (94%) and the level of genetic variation among populations was very low (6%). High level of genetic variation within populations can be justified by highest amount of gen flow (Nm= 4.576).

https://ijpps.ut.ac.ir/article_54731_b955eb4ad82bc67b50dd99da39d03e65.pdf