University of Tehran Iranian Journal of Plant Protection Science 2008-4781 50 1 2019 05 22 Study on shallow bark canker disease of walnut in Kerman province Study on shallow bark canker disease of walnut in Kerman province 107 117 72573 10.22059/ijpps.2019.226410.1006759 FA Elahe Sadeghi M. Sc. Student, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran Pejman Khodaygan Associate Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran Ahmad Hosseini Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran Roohallah Saberi-Riseh Associate Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Iran Journal Article 2017 01 30 Walnut is one of the important nuts with a high economic value. Shallow bark canker, caused by Brenneria nigrifluens, is one of the most dangerous diseases of the walnut trees. Early detection of the causal agent has an important role in preventing economic and irreparable losses caused by this disease. In this study, 57 bacterial strains were isolated from walnut trees with the typical lesions of the shallow bark cankers from different regions of Kerman province. These isolates were divided into two groups, based on phenotypic and biochemical characteristics and then, amplification product of the expected size (255 bp) was obtained with genomic DNA using specific pair primers in B. nigrifluens isolates.Pathogenicities of the isolates were assessed on immature walnut fruits. To identify the causal and the associated bacteria molecularly, a part of 16s rDNA gene sequence was analyzed. Results showed that B. nigrifluens with a significant genetic diversity spread over different regions of Kerman province. In addition, the genus Gibbsiella can probably contribute to the increase of the disease severity, which requires further studies. Walnut is one of the important nuts with a high economic value. Shallow bark canker, caused by Brenneria nigrifluens, is one of the most dangerous diseases of the walnut trees. Early detection of the causal agent has an important role in preventing economic and irreparable losses caused by this disease. In this study, 57 bacterial strains were isolated from walnut trees with the typical lesions of the shallow bark cankers from different regions of Kerman province. These isolates were divided into two groups, based on phenotypic and biochemical characteristics and then, amplification product of the expected size (255 bp) was obtained with genomic DNA using specific pair primers in B. nigrifluens isolates.Pathogenicities of the isolates were assessed on immature walnut fruits. To identify the causal and the associated bacteria molecularly, a part of 16s rDNA gene sequence was analyzed. Results showed that B. nigrifluens with a significant genetic diversity spread over different regions of Kerman province. In addition, the genus Gibbsiella can probably contribute to the increase of the disease severity, which requires further studies.

https://ijpps.ut.ac.ir/article_72573_030cc22d0ee27512770fd7cb7d65a031.pdf