Genetic Diversity of Chilo supperssalis(Lepidoptera: Pyralidae) Populations Based on RAPD Marker in Guilan Province, Iran

Farahpor Haghanni, Atusa; Hoseini, Reza; Ebadi, Ali Akbar; Aelami, Ali; Majidi Shilsar, Farzad

doi:10.22059/ijpps.2014.36684

Genetic Diversity of Chilo supperssalis(Lepidoptera: Pyralidae) Populations Based on RAPD Marker in Guilan Province, Iran

Document Type : Research Paper

Authors

Atusa Farahpor Haghanni ¹

Reza Hoseini ²

Ali Akbar Ebadi ³

Ali Aelami ²

Farzad Majidi Shilsar ⁴

¹ FormerMSc.Student, Faculty of Agriculture, University of Guilan, Iran

² Assistant Professor, Faculty of Agriculture, University of Guilan, Iran

³ Instructor, Rice Research Institute, Amol, Iran

⁴ Assistant Professor, Rice Research Institute, Rasht, Iran

10.22059/ijpps.2014.36684

Abstract

To investigate the genetic diversity of Chilo suppressalis populations in Guilan province,
a number of 37 of samples (4-6th larva stages) were collected from 17 different regions of
the province. The collected samples were parted into three population groups coming
from west, center and east parts of Guilan. Extracted DNAs were polymerized through 12
RAPD markers. RAPD-PCR products were electrophoresed on a 1.5% agarose gel and
photographed through GelDoc. Following bands scoring, analysis of data revealed that
the 12 primers produced a total of 112 polymorphic bands. Results indicated that C.
suppressalis population differs from the other two populations. Center and east Guilan
populations demonstrated more similarities to each other. This could be due to an
occurrence of gene-flow between the two populations causing the similarities. The
population coming from the center of Guilan demonstrated a highest level of
intrapopulation genetic variation due to a wider activity area and as well due to a higher
number of the studied samples. These results finally indicated that population’s active in
the province lack homogeny and are comprised of two different biotypes. In total, the
results could be considered as an explanation for morphological and biochemical
differences found among the active biotypes within the studied areas.

Keywords

Biotype

electrophoresis

Genetic variation

marker

RAPD-PCR