SCREENING OF TRICHOTHECENE -PRODUCing FUSARIUM CULMORUM ISOLATES FROM WHEAT FARMS OF WEST AZARBAYJAN PROVINCE ACCORDING TO THE TRI13 GENE

Lava, Azad; Salari, Mohammad

doi:10.22059/ijpps.2014.52250

SCREENING OF TRICHOTHECENE -PRODUCing FUSARIUM CULMORUM ISOLATES FROM WHEAT FARMS OF WEST AZARBAYJAN PROVINCE ACCORDING TO THE TRI13 GENE

Document Type : Research Paper

Authors

Azad Lava

Mohammad Salari

nothing

10.22059/ijpps.2014.52250

Abstract

Fusarium culmorum which is considered as the causal agent of Fusarium head blight, can produce deoxynivalenol (DON), nivalenol (NIV) and their acetylated derivatives. This study has been assessed in order to detect the Tri13 gene using primer pairs Tri13DONR/Tri13F and Tri13R/Tri13NIVF, to investigate the potential of F. culmorum isolates in the production of DON and NIV in West Azarbayjan province, Iran. In this study, 98 F. culmorum isolates were obtained from Urmia, Khoy, Miandoab, Maku, Tekab and Mahabad regions. For this purpose, F. culmorum isolates were identified based on the morphological characteristics as well as specific primer pairs, C51F/C51R. Production of DON and NIV were detected in these isolates according to Tri13 gene. Results showed that 60.2 percent of isolates have the potential to produce DON and 39.8 percent of isolates have ability to produce NIV.

Keywords

Deoxynivalenol

fusarium head blight

Nivalenol

tri13

Wheat