Iranian Journal of Plant Protection Science

Iranian Journal of Plant Protection Science

The effect of arbuscular mycorrhizae on Verticillium wilt of pistachio rootstocks Ahmad Aghaei and Badami Zarand: growth, nutritional and biochemical characteristics

Document Type : Research Paper

Authors
sakineh jamali paghaleh 1
Naser Radman 2
Amir Hossein Mohammadi 3
Mahdi Pirnia 2
Abdolhosein Taheri 4
1 ِPh. D. student, Department of Plant Pathology, Faculty of Agriculture, University of Zabol, Iran
2 Associate Professor, Department of Plant Pathology, Faculty of Agriculture, University of Zabol, Iran
3 Assistant Professor, Pistachio Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran
4 Associate Professor, Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Iran
10.22059/ijpps.2022.348955.1007010
Abstract
Verticillium wilt is one of the important diseases that causes economic damage to pistachio trees. Arbuscular mycorrhizae in addition to improvement of plant growth, can increase tolerance to plant pathogens. In this research, the effect of mixture of three species of arbuscular mycorrhizal fungi, Funneliformis mosseae, Rhizophagus irregularis and Claroideoglomus etunicatum was evaluated on Verticillium wilt of pistachio rootstocks Ahmad Aghaei and Badami Zarand (susceptible and resistant to Verticillium dahliae, respectively). Arbuscular mycorrhizae (AM) inoculation was done at the time of sowing pistachio seeds and pathogen (Vd) inoculated after 52 days using amended inoculum on sand-barley flour-distilled water substrate. The experiment was performed as factorial in completely randomized design with 5 replications in greenhouse conditions. The results showed that the inoculation of AM increases shoot and root dry weight, stem height and diameter, leaf area, concentration of nutrients, proline, soluble sugars, chlorophyll comparison with the control and inoculated plants with the pathogen. In AM+Vd treatment, the presence of the pathogen only in Ahmad Aghaei rootstock caused a decrease in the colonization percentage of arbuscular mycorrhizal fungi comparison with the AM treatment. At the end of the experiment, the pathogenicity index in the Vd treatment was 3.9 and 1.9 in Ahmad Aghaei and Badami Zarand rootstocks respectively but in the AM+Vd treatment it reached to 3.6 and 1.1, which showed a significant decrease in disease severity. It is concluded that inoculation of AM by improving the growth, nutritional and biochemical characteristics can increase resistance of pistachio seedlings to Verticillium wilt.
Keywords
Biological control
Chlorophyll
Proline
Mineral elements
Verticillium dahliae
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Iranian Journal of Plant Protection Science
Volume 53, Issue 2
January 2023
Pages 239-254

  • Receive Date 04 October 2022
  • Revise Date 26 October 2022
  • Accept Date 07 November 2022