Document Type : Research Paper
Authors
1
Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2
Associate Professor of Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3
Department of Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran
4
Professor of Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Abstract
In order to identification of endophytic fungi of damask rose (Rosa damascena Mill.) in Iran, sampling during the years 2017 and 2018 from healthy branches and leaves in the provinces of East Azarbaijan, West Azarbaijan, Isfahan, Alborz, Khorasan Razavi, Zanjan, Kerman, Kermanshah, Mazandaran, and Yazd were done. A total of 58 fungal isolates were identified using the morphological characteristics and molecular analysis based on ITS-rDNA regions and part of tef1-α gene. In this study, Fusarium globosum, Sarocladium kiliense, Colletotrichum karsti, Lophotrichus ampulus, Ascotricha chartarum, Hyphodontia setulose, and Kalmusia variispora were identified. The taxa obtained in this research are reported for the first time in the world as endophytic fungi from the damask rose. Lophotrichus ampullus is a new taxon for the funga of Iran. The pathogenicity test of the selected isolates was performed on the seedlings obtained from tissue culture of the rose by creating wounds on the branch. Evaluation of the results after 25 days showed that the isolates used on this plant are not pathogenic. The screening of endophytic fungal isolates was done based on the amount of total phenolics and flavonoids production in the culture medium using spectrophotometric method. The results showed that the two isolates Rd45 (L. ampullus) and Rd393 (K. variispora) have the highest and lowest amount of total flavonoids, respectively. In addition, two isolates Rd36 (H. setulosa) and Rd45 also produced the highest and lowest amount of total phenol, respectively.
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