Investigation of the interaction of Bacillus velezensis and Trichoderma harzianum in controlling Fusarium wilt of potatoes caused by Fusarium oxysporum f. sp. tuberosi

Articles in Press

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Plant Protection Research Department, Khorasan Razavi Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

10.22059/ijpps.2025.402124.1007092

Abstract

The biological control of Fusarium wilt in potato, which is caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. tuberosi, is essential for sustainable agriculture. Therefore, the present study was conducted to investigate the interaction between two beneficial microorganisms, the bacterium Bacillus velezensis UTB96 and the fungus Trichoderma harzianum T22, in controlling Fusarium wilt in potato plants in vitro and in vivo conditions. The experiment was designed using a completely randomized design to ensure accurate and reliable results. The results showed that in vitro conditions, the combined treatment of B. velezensis + T. harzianum inhibited the pathogen's growth by 77.73%, while individual treatments of B. velezensis and T. harzianum inhibited fungal growth by 40.12% and 70.12%, respectively. In greenhouse conditions, the combined use of these two microorganisms outperformed the individual treatments. The combination of B. velezensis and T. harzianum controlled Fusarium wilt by 76.75% and significantly enhanced plant height, fresh and dry weights of different parts of the potato plant. In comparison, B. velezensis and T. harzianum alone controlled the disease by 62.3% and 57.5%, respectively. Thus, although the individual isolates of B. velezensis and T. harzianum can partially control the disease, this study demonstrated that their combined application is more effective to control Fusarium wilt of potato.

Keywords

Iranian Journal of Plant Protection Science

Articles in Press, Accepted Manuscript
Available Online from 13 December 2025

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History

  • Receive Date: 13 September 2025
  • Revise Date: 22 November 2025
  • Accept Date: 13 December 2025

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