Role of Bacillus subtilis Volatiles in Induction of Systemic Resistance in Arabidopsis

Document Type : Research Paper

Authors

1 PhD. Student, University College of Agriculture and Natural Resources, Universiyt of Tehran

2 Associate Professor University College of Agriculture and Natural Resources, Universiyt of Tehran

3 Professor, Industrial Biotechnology Research Institute, South Korea

Abstract

Biological control agents cause induction of systemic resistance through an array of elicitors. In this work, the role of some synthetic compounds in Bacillus subtilis GB03 volatiles, profile was assessed on either direct inhibition or induction of systemic resistance against B. cinerea in Arabidopsis. In comparison with control, all the compounds not only did not suppress fungal sporulation but also increased it significantly. Maximum effects on sporulation were obtained by 3-pentanol and methyl salicylate with 4.7 ×105 and 4 ×105 spore/ml, respectively. Most of the compounds promoted fungal growth, the highest rates of which were  recorded in case of methyl jasmonate and methyl salicylate with respective 23 and 24 percent. Interestingly, minimum vs. maximum of pigment production was also recorded for methyl jasmonate and methyl salicylate, respectively. In induction of resistance experiment, 3- hydroxy-2 butanol, methyl jasmonate, butanediol, acetone and 1-pentanol exerted the highest effect in suppression of disease on Arabidopsis. Methyl salicylate and 3-pentanol,  known for their stimulation of defense against biotrophs did not show any noticeable effect in suppression of this necrotrophic pathogen. Eventually, efficiency of the compounds is dependent upon their doses and in low dose they mainly act as signalling molecule rather than direct inhibitor of fungi.
 

Keywords

Files

History

  • Receive Date: 21 August 2012
  • Revise Date: 25 November 2013
  • Accept Date: 28 May 2013

Share

How to cite

Statistics