Effect of Culture Substrates on Virulence of Metarhizium anisopliae Conidia and Blastospores against Sunn Pest, Eurygaster integriceps

Roshandel, Siyamak; Talaei-Hassanlouei, Reza; Askary, Hasan; Allahyari, Hossein; Marzban, Rasoul

doi:10.22059/ijpps.2014.36668

Effect of Culture Substrates on Virulence of Metarhizium anisopliae Conidia and Blastospores against Sunn Pest, Eurygaster integriceps

Document Type : Research Paper

Authors

Siyamak Roshandel ¹

Reza Talaei-Hassanlouei ²

Hasan Askary ³

Hossein Allahyari ²

Rasoul Marzban ³

¹ PhD Student, College of Agriculture andNatural Resources,University of Tehran, Tehran, Iran

² Associate Professor,College of Agriculture andNatural Resources,University of Tehran, Tehran, Iran

³ Associate Professor, Research InstituteofPlant Protection,Tehran, Iran

10.22059/ijpps.2014.36668

Abstract

Virulence of blastospores and conidia of the fungus Metarhizium anisopliae were investigated
on different instar nymphs and adults of Sunn pest, Eurygater integriceps. Experiments were
carried out through a factorial arrangement in a completely randomized design of three
replications. The bioassays were performed through immersion method. Results revealed
significant differences among virulence of blastospores and of conidia produced in different
media.The highest vs the lowest total mortality percentages of the second instar nymphs were
recorded as 100 and 41.6% on rice bran vs wheat bran+ rice bran media respectively in
blastopore treatments. As for conidia, the highest total mortality percentage of second instar
nymphs was 100% on wheat bran, Behnoush waste barley and rice flour media whilst the
lowest total mortality percentage recorded as 86.1% on maize medium. Minimum LT50 was
recorded (2.3 days) on wheat bran + yeast extract medium in the second instar nymphs whilst
the highest LT50 recorded (10.1 days) on wheat bran+ rice bran extract medium for the fourth
instar nymphs in blastopore treatments. Minimum LT50 was recorded (4.7 days) on the rice
bran medium and at second instar nymphs while the highest recorded (13.1 days) for the fourth
instar nymphs by conidia harvested from non-broken rice medium.

Keywords

Culture substrate

Metarhizium anisopliae

Sunn pest

virulence