Genetic diversity of fluorescent pseudomonads isolated from wheat fields with biocontrol ability against Gaeumannomyces graminis var. tritici

Shirzad, Akbar; Fallahzade Mamaghani, Vahid; Pajouhande, Maghsoud; Alizade, Alireza

doi:10.22059/ijpps.2014.52254

Genetic diversity of fluorescent pseudomonads isolated from wheat fields with biocontrol ability against Gaeumannomyces graminis var. tritici

Document Type : Research Paper

Authors

Akbar Shirzad ¹

Vahid Fallahzade Mamaghani ¹

Maghsoud Pajouhande ¹

Alireza Alizade ²

¹ Assistant Professor/Azarbayjan Shahid Madani University

² PhD Candidate, University of Tehran, Iran

10.22059/ijpps.2014.52254

Abstract

In this research different isolates of fluorescent pseudomonads was provided and after investigation of their biochemical characteristics, and their ability to control take-all disease of wheat, their genetic diversity and relation between DNA fingerprinting and biocontrol ability of the isolates was investigated using rep-PCR molecular marker. DNA fingerprinting results and the drown dendrogram showed that19 fluorescent pseudomonads isolates differentiated in 25-100% genetic similarity level and the isolates belonged to nine fingerprinting groups assigned by letters A-I. Based on these results, there was a relation between DNA fingerprinting and inhibition zone, production of HCN and diacetylphloroglucinol production bioassays. Therefore, we suggested that rep-PCR molecular marker is a useful tool for identification and screening of fluorescent pseudomonads with biocontrol ability.

Keywords

take-all disease of wheat

Genetic diversity

Pseudomonas flourescens

biological control