Genetic diversity of fluorescent pseudomonads isolated from wheat fields with biocontrol ability against Gaeumannomyces graminis var. tritici

Document Type : Research Paper

Authors

1 Assistant Professor/Azarbayjan Shahid Madani University

2 PhD Candidate, University of Tehran, Iran

Abstract

In this research different isolates of fluorescent pseudomonads was provided and after investigation of their biochemical characteristics, and their ability to control take-all disease of wheat, their genetic diversity and relation between DNA fingerprinting and biocontrol ability of the isolates was investigated using rep-PCR molecular marker. DNA fingerprinting results and the drown dendrogram showed that19 fluorescent pseudomonads isolates differentiated in 25-100% genetic similarity level and the isolates belonged to nine fingerprinting groups assigned by letters A-I. Based on these results, there was a relation between DNA fingerprinting and inhibition zone, production of HCN and diacetylphloroglucinol production bioassays. Therefore, we suggested that rep-PCR molecular marker is a useful tool for identification and screening of fluorescent pseudomonads with biocontrol ability.

Keywords

Iranian Journal of Plant Protection Science
Volume 45, Issue 1 - Serial Number 1
Spring and Summer
May 2014
Pages 121-138

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History

  • Receive Date: 21 November 2013
  • Revise Date: 15 February 2014
  • Accept Date: 28 April 2014

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