Gene ontology of Trichoderma harzianum differential ESTs during colonization of tomato spermosphere and rhizoplane

Mehrabi-Koushki, Mehdi; Rouhani, Hamid; Mahdikhani Moghaddam, Esmat

doi:10.22059/ijpps.2014.52258

Gene ontology of Trichoderma harzianum differential ESTs during colonization of tomato spermosphere and rhizoplane

Document Type : Research Paper

Authors

Mehdi Mehrabi-Koushki

Hamid Rouhani ¹

Esmat Mahdikhani moghaddam ²

¹ Professor of Ferdowsi University of Mashhad

² Ferdowsi University of mashhad

10.22059/ijpps.2014.52258

Abstract

Some Trichoderma strains are used as biocontrol agents against phytopathogens. Differential display reverse transcriptase PCR has been developed to detect differentially expressed genes of T. harzianum T7 during colonization stages of tomato spermosphere and rhizoplane. DDRT-PCR products were diplayed on gel and 62 differential bands excised, purified, cloned, and sequenced. Obtained ESTs were submit-queried to NCBI database by BLAST2GO search and evaluated their gene ontology. Most of the transcripts corresponded to known or hypothetical proteins. These proteins correspond to different functional groups, which play role in metabolism, signaling both within and between cells, host interaction, protein transport, translation, controlling of cell growth and survival, adaptation to environmental variables, transportation of biomolecules between nucleus and cytoplasm, regulating protein function, cell division, catalyzing analogous reactions, repairing damaged DNA molecules, and other vital functions in different organisms. Some of the detected ESTs in this study corresponded to genes encodes enzymes potentially involved in nutritional support of Trichoderma in its ecological niches.

Keywords

BLAST2GO

ESTs

DDRT-PCR and Gene Function