Biochemical characterization of α- amylase in melon ladybird, Epilachna chrysomelina (F.) and inhibitory effects of inhibitors extracted from plant seeds on its activity

Document Type : Research Paper

Authors

1 Former M. Sc. Student, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan

2 Associate Professor, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan

3 Associate Professor, Department of Biochemistry, Faculty of Sciences, University of Tarbiat Modares, Tehran

Abstract

Epilachna chrysomelina is one of major pests of cucurbitaceous plants. Biochemical properties of α- amylase were studied in melon ladybird. The optimal pH for α- amylase was found at 4. Also, high amylolytic activity was found at temperature 50 °C. The specific activity of this enzyme in digestive system of different larval instar, foregut, midgut, hindgut and female and male adults were determined. Results showed that the highest specific α- amylase activity was detected in the digestive system of 3rd instar larvae and the enzyme activity in males and females digestive system did not show any significant difference. The enzyme specific activity in midgut was 5- and 8.3-fold higher than foregut and hindgut. The Michaelis–Menten constant (Km) of α-amylase was obtained as 0.69 mg/ml.  Zymogram analysis of α- amylase showed one isoform. Also, inhibitory effect of inhibitors extracted from seeds of Lathyrus sativus, Trifolium alexandrium, Zea mays, Faba vulgaris, Lentis culinaris, Vigna unguiculata, Phaseolus vulgaris and Vigna radiate were evaluated on E. chrysomelina α- amylase activity. Among these inhibitors, the inhibitors extracted P. vulgaris and V. radiate were able to inhibit E. chrysomelina amylase.

Keywords

References

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Iranian Journal of Plant Protection Science
Volume 45, Issue 2 - Serial Number 2
Autumn & Winter
May 2015
Pages 251-263

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History

  • Receive Date: 12 October 2013
  • Revise Date: 27 September 2014
  • Accept Date: 24 December 2014

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