Iranian Journal of Plant Protection Science

Iranian Journal of Plant Protection Science

Effect of an extracted lectin from Sclerotinia sclerotiorum de Bary on mortality and physiological parameters in the larvae of large cabbage butterfly, Pieris brassicae (Lepidoptera: Pieridae)

Document Type : Research Paper

Authors
Zeynab Alborzi 1
Arash Zibaee 2
Jalal Jalali Sendi 3
Azadeh Karimi Malati 2
Babak Rabiee 4
Mehdi Salimi 5
1 M. Sc. Student, Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Iran
2 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Iran
3 Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Iran
4 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Guilan, Rasht, Iran
5 Library Expert
10.22059/ijpps.2015.54720
Abstract
In the current study, the extracted lectin from plant pathogenic fungus, Sclerotinia sclerotiorum was added to diet of third larval instars of Large cabbage butterfly, then its effects were determined on mortality, feeding and intermediary metabolism. Concentrations of 0.5, 1 and 2 mg/ml of the lectin caused 20-60% of mortality in the treated larvae. Amounts of nutritional indices in the treated larvae significantly decreased versus control although level of Metabolic cost in the treated larvae showed statistical increase versus control. Activities of the digestive enzymes such as alpha-amylase, glucosidases, lipase, general and specific proteases (Serine and Exopeptidases) decreased in the fed larvae on lectin concentrations versus control. Although activities of aspartate aminotransferase and γ-glutamyl transferase significantly increased in the concentrations of 1 and 2 mg/ml but activity of alanine aminotransferase had a significant decrease versus control. Activity of acid phosphatase had no statistical differences among treatments and control but activity of alkaline phosphatase was significantly lower than control larvae. Activity of lactate dehydrogenase in the fed larvae on the concentration of 2 mg/ml had significant increase but activities of aldolase in the control and fed larvae on 0.5 mg/ml of lectin were higher than those of other treatments. Amounts of storage macromolecules such as protein, triglyceride and glycogen in the fed larvae on the lectin were lower than those of control so that it was more significant in the concentration of 2 mg/ml. Results of the current study demonstrated that the lectin from S. clerotiorumcaused mortality and interference in digestive physiology and intermediary metabolism of P. Brassicae larvae so it could be used as a toxic molecule to control of the pest.
Keywords
Lectin
Sclerotinia sclerotiorum
Pieris brassicae
Digestive physiology
Intermediary metabolism
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Iranian Journal of Plant Protection Science
Volume 46, Issue 1 - Serial Number 1
Spring & Summer
May 2015
Pages 41-57

  • Receive Date 19 November 2014
  • Revise Date 27 February 2015
  • Accept Date 16 June 2015