Imidacloprid resistance in Thrips tabaci (Thys. Thripidae) populations collected from onion fields in Isfahan

Document Type : Research Paper

Authors

1 Former Graduate Student, Entomology, Department of Plant Protection, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran

2 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran

Abstract

Onion thrips, Thrips tabaci, is the most important pest of onion in Isfahan. Onion growers often rely on the pesticide spraying. Imidacloprid is frequently used against this pest. To investigate the thrips resistance to this insecticide, 10 onion-growing regions of Isfahan were selected. After their laboratory rearing, certain concentrations of imidacloprid were applied to these populations, using a leaf dip bioassay method. LC50 values of field populations were compared to Harand population as a susceptible reference strain. Different levels of resistance were observed in most populations. To investigate the resistance mechanisms, populations were pre-treated with two synergists namely PBO (piperonyl butoxide) and DEM (diethyl maleate) as inhibitors of monooxygenases and glutathione-S-transferases, respectively.  Based on the results, theses enzymes do not seem to play a substantial role in the resistance. The results also indicated that there is no high level of cross resistance in most imidacloprid-resistant populations against acetamiprid, another neonicotinoids insecticide

Keywords


  1. Bielza, P. (2008). Insecticide resistance management strategies against the western flower thrips, Frankliniella occidentalis. Pest Management Science, 64(11), 1131-1138.
  2. Bielza, P., Espinosa, P., Quinto, V., Abellan, J. & Contreras, J. (2007). Synergism studies with binary mixtures of pyrethroid, carbamate and organophosphate insecticides on Frankliniella occidentalis (Pergande). Pest Management Science, 63(7), 84-89.
  3. Broadbent, A. & Pree, D. (1997). Resistance to insecticides in populations of Frankuniella occidentalis (Pergande) (Thysanoptera: Thripidae) from greenhouses in the Niagara region of Ontario. The Canadian Entomologist, 129(5), 907-913.
  4. Burnstone, J. A. (2009). Investigations into the Biology and Behaviour of Thrips tabaci L. UK: University of Warwick.
  5. Capinera, J. (2001) Handbook of Vegetable Pests. California: Academic Press.
  6. Cifuentes, D., Chynoweth, R., Guillen, J., De La Rua, P. & Bielza, P. (2012). Novel cytochrome P450 genes, CYP6EB1 and CYP6EC1, are over-expressed in acrinathrin-resistant Frankliniella occidentalis (Thysanoptera: Thripidae). Journal of Economic Entomology, 105(3), 1006-1018.
  7. Crossthwaite, A. J., Rendine, S., Stenta, M., & Slater, R. (2014). Target-site resistance to neonicotinoids. Journal of chemical biology, 7(4), 125-128.
  8. Diaz-Montano, J., Fuchs, M., Nault, B. A., Fail, J., & Shelton, A. M. (2011). Onion thrips (Thysanoptera: Thripidae): A global pest of increasing concern in onion. Journal of Economic Entomology, 104(1), 1-13.
  9. Espinosa, P. J., Bielza, P., Contreras, J. & Lacasa A. (2002). Field and laboratory selection of Frankliniella occidentalis (Pergande) for resistance to insecticides. Pest Management Science, 58, 920-927.
  10. Espinosa, P. J., Contreras, J., Quinto, V., Gravalos, C., Fernandez, E. & Bielza, P. (2005). Metabolic mechanisms of insecticide resistance in the western flower thrips, Frankliniella occidentalis (Pergande). Pest Management Science, 61, 1009-1015.
  11. Feng, Y., Wu, Q., Wang, S., Chang, X., Xie, W., Xu, B. & Zhang, Y. (2010). Cross-resistance study and biochemical mechanisms of thiamethoxam resistance in B-biotype Bemisia tabaci (Hemiptera: Aleyrodidae), Pest Management Science, 66, 313-318.
  12. Foster, S. P., Gorman, K. & Denholm, I. (2010). English field samples of Thrips tabaci show strong and ubiquitous resistance to deltamethrin. Pest Management Science, 66(8), 861-864.
  13. Gao, C. F., Ma, S. Z., Shan, C. H. & Wu, S. F. (2014). Thiamethoxam resistance selected in the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae): Cross-resistance patterns, possible biochemical mechanisms and fitness costs analysis. Pesticide Biochemistry and Physiology, 114, 90-96.
  14. Gao, Y., Lei, Z. & Reitz, S. R. (2012). Western flower thrips resistance to insecticides: detection, mechanisms and management strategies. Pest Management Science, 68(8), 1111-1121.
  15. Herron, G. A., James, T. M., Rophial, J. & Mo, J. (2008). Australian populations of onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), are resistant to some insecticides used for their control. Australian Journal of Entomology, 47(4), 361-364.
  16. Herron G. A., Rophail, J., James, T. M. (2006). A method to bioassay onion thrips, Thrips tabaci Lindeman (thysanoptera: Thripidae) for pesticide response. Genetic and Applied Entomology, 35, 15-19.
  17. Jensen, S. E., (2000). Insecticide resistance in the western flower thrips, Frankliniella occidentalis. Integrated Pest Management Review, 5, 131-146.
  18. Lewis, T., (1997). Field and Laboratory Techniques. In Lewis, T. (Ed.). Thrips as crop pests. (pp. 435-475). CAB, Wallingford, Oxon, UK.
  19. Martin, N., Workman, P., & Butler, R., (2003). Insecticide resistance in onion thrips (Thrips tabaci) (Thysanoptera: Thripidae). New Zealand Journal of Crop and Horticultural Science, 31(2), 99-106.
  20. Martin, N., Workman, P., & Butler, R. (2005). Insecticide bioassays for western flower thrips (Frankliniella occidentalis) (Thysanoptera: Thripidae) and greenhouse whitefly (Trialeurodes vaporariorum) (Hemiptera: Aleyrodidae). New Zealand Journal of Crop and Horticultural Science, 33(2), 177-184.
  21. Martin, N., Workman, P. J., Hedderly, D. and Fagan, L. L. (2008). Monitoring onion (Allium cepa) crops for onion thrips (Thrips tabaci) (Thysanoptera: Thripidae): testing a commercial protocol. New Zealand Journal of Crop and Horticultural Science, 36(2), 145-152.
  22. Modarres Avval, M. (2011). List of agricultural pests and their natural enemies. Mashhad: Ferdowsi University Press. (In Farsi).
  23. Nauen, R. and Denholm I. (2005). Resistance of insect pests to neonicotinoid insecticides: current status and future prospects. Archives of Insect Biochemistry and Physiology, 58(4), 200-215.
  24. Richardson, B. & Wene, G. (1956). Control of onion thrips and its tolerance to certain chlorinated hydrocarbons. Journal of Economic Entomology, 49(3), 333-335.
  25. The Arthropod Pesticide Resistance Database (ARPD) (2014). available in http://www.irac-online.org.
  26. Yu, S. J. (2008). The toxicology and biochemistry of insecticides. CRC Press, Taylor & Francis Group. Boca Raton, FL.
  27. Zhao, G., Liu, W. E. I., Brown, J. M. & Knowles, C. O. (1995). Insecticide resistance in field and laboratory strains of western flower thrips (Thysanoptera: Thripidae). Journal of Economic Entomology, 88(5), 1164-1170.
  28. Zewen, L., Zhaojun, H., Yinchang, W., Lingchun, Z., Hongwei, Z. & Chengjun L. (2003). Selection for imidacloprid resistance in Nilaparvata lugens: cross-resistance patterns and possible mechanisms. Pest Management Science, 59(12), 1355-1359.