Evaluation of diversity among some melon (Cucumis melo) accessions for resistance to fusarium wilt based on biochemical enzymes activity

Document Type : Research Paper

Authors

1 . Former M.Sc. Student , Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Associate Professor, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 . Professor, Seed and Plant Improvement Institute

Abstract

Fusarium wilt is a restrictive disease of melons in the world and Iran. In this research, fifty seven accessions of Cucumis melo, planted in a randomized complete block design with three replications. The relationship between resistance and changes in biochemical activity were evaluated for these accessions. The wounded root of seedlings after reaching at one to two true leaf stage were placed in inoculum concentrations of 1×106 spores ml-1 of Fusarium oxysporum f. sp. melonis for 3-4 min and then they were transferred to cultivation trays. Area under disease progress curve (AUDPC), disease severity and five physiological traits were measured. Analysis of variance showed significant differences among genotypes for all traits. The correlation coefficient between different traits showed that the AUDPC and disease severity have a negative correlation with Peroxidase, Polyphenol oxidase, Phenol Compound, Superoxide dismutase and Catalase. The grouping results of genotypes based on squared Euclidean distance measurement and Ward method were clustered these accessions in three groups; resistant, moderately resistant and susceptible. In this study the farthest distance (28.67) was observed between Isabelle and Shadegani2. These accessions recognized as the most resistant and susceptible genotypes, respectively. It seems that due to the distance between them, with make crossing and use of heterosis, we may achieve to progeny that are more resistant in comparing to the evaluated genotypes of this study.
 
 
 

Keywords

References

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Iranian Journal of Plant Protection Science
Volume 46, Issue 2 - Serial Number 2
Autumn & Winter
October 2015
Pages 191-205

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History

  • Receive Date: 20 August 2014
  • Revise Date: 06 September 2015
  • Accept Date: 14 September 2015

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