The combined effect of root-knot nematode and broomrape on tomatoes chlorophyll content, fluorescence parameters and relative water content under greenhouse conditions

Document Type : Research Paper

Authors

1 M.Sc. Student, Plant Protection Department, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 , Assistant Professor , Plant Protection Department, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Ph.D. Student, Plant Protection Department, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

Different species of the root knot nematodes (Meloidogyne spp.) and broomrape Orobanche aegyptica are parasitic of different plants roots. In order to study the interaction between the parasites of tomato, an experiment was conducted in a completely randomized design with four treatments 1- control, 2- nematode (2000, second stage juveniles), 3- seeds of orobanche (30 mg per kg of soil) and 4- nematode (2000, second stage juveniles), with seeds of orobanche (30 mg per kg of soil) with four replications under greenhouse conditions. Treated seeds were planted in sterilized soil. Four-leaf stage seedlings were inoculated with treatments and transferred to pots and fluorescence parameters were measured after 60 days. The results showed that both biological stresses caused a significant reduction in shoot dry weight, number of galls, chlorophyll, leaf number, leaf area, plant height, root length, stem diameter and photosystem II quantum efficiency in both light and darkness compared with control. Also the number of second stage larvae, egg sacs, accessories orobanche, nematode reproductive factor, the M, F, QP, QN, NPQ, Fo, Fm and Fv of both light and darkness in the combined treatment were increased. Whereas the dry weight of root in nematodes tension increased and were decreased in stress of aegyptian orobanche. The root volume was highest in alone nematode treatment. The results indicated that root- knot nematode and orobanche had a synergistic effect.
 

Keywords

References

  1.  

    1. Arraus, S.L., Amaro, T.V., Has, J., Nakkoal, H. & Nachit, M.M. (1998). Chlorophyll fluorescence as a selection criterion for gain yield in durum wheat under Medditerranean condition. Field Crops Research, 55, 202-223.
    2. Ashraf, M. & Harris, P.J.C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Sciences, 166, 3-16.
    3. Audebert, A., Coyne, D.L., Dingkuhn, M. & Plowright, R.A. (2000). The influence of Cyst Nematodes (Heterodera sacchari) and drought on water relation and growth of upland rice in Coted Ivoire plant and soil. Journal of Nematology, 235-242P.
    4. Baker, N. & Rosenqvist, E. (2004). Application of chlorophyll fluorescence can improve crop production strategies: An examination of future possibilities. Journal of Experimental Botany, 55, 1607-1621.
    5. Barker, K.R., Carter, C.C. & Sasser, J.N. (1985). An advanced treatise on Meloidogyne. Plant Pathology, pp: 212-233.
    6. Berger, S., Benediktyova, Z., Matous, K., Bonfig, K., Muler, M.J., Nedbal, L. & Roitsch, T. (2007). Visualization of dynamics of plant-pathogen interaction by novel combination of chlorophyll fluorescence imaging and statistical analysisi: differential effects of virulent and avirulent strains of P. syringae and of oxylipins on A. thaliana. Journal of Experimental Botany, 58(4), 797-806.
    7. Ellis, M.A., Ferree, D.C. & Spring, D.E. (1981). Photosynthesis, transpiration, and carbohydrate content of apple leaves infected by Podosphaera leucotricha, Phytopathology, 71, 392-395.
    8. Faostat. Fao.org/site/default.aspx.
    9. Francheboud, Y. & Leipner, J. (2003). The application of chlorophyll fluorescence to study light, temperature and drought stress. In: Dw-Ell, J.R., P.M.A. Tiovonen (eds.). Practical application of chlorophyll fluorescence in plant biology. Boston: Kluwer Academic Publisheres, Pp: 125-150.
    10. Gabriels, R., Keirsblulk, W.V. & Engels, H. (1993). A rapid method for the determination of physical propertits of growing media. Acta Horticulturae, 342, 243-247.
    11. Gharabadiyan, F., Jamali, S., Ahmadiyan Yazdi, A. & Eskandari, A. (2012). Source of resistance to root knot nematode (Meloidogyne javanica) in tomato cultivars. Journal of Agricultural Technology, 2011-2021.
    12. Hagigi, H., Taheri, A., Razavi, S.A., Tanha maafii, Z. & Mamagani, M. (2008). Investigation greenhouse of Interaction Race two Root-Knot Nematode Meloidogyne incognita and Verticillum dahliae agent verticillium wilt olive (Olea europaea) Seedlings in Gorgan. Journal Agriculture Sciences and Natural Resource, 15(4). (in Farsi)
    13. Hartman, K.M. & Sasser, J.N. (1985). Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. In: An advanced tretise on meloidogyne. Vol, 2, methodology, Barker, K.R., C.C. Carter., and J.N. Sasser. Eds. Relrigh, NC, USA: NCSU Graphics. P: 69-77.
    14. Hosseini Nejad, S.A. & Khan, M.W. (2001). Interaction of root-knot nematode, Meloidogyne incognita (race 1) on chick–pea cultivars. Applied Entomology and Phytopathology, 68, 1-11.
    15. Hussain, M.A., Mukhtar, T. & Kayani, M.Z. (2011). Assessment of the damage cased by Meloidogyne incognita on OKRA (Abelmoschus esculentus). The Journal of Animal and Plant Sciences, 21(4), 857-861.
    16. Hussey, R.S. & Barker, K.R. (1973). A comparison of methods of collecting inocula of Meloidogyne spp. Including a new technique, Plant Disease Reporter, 57, 1025-1028.
    17. Izadpanah, K.A., Aahkan, S.M., Banihashemi, Z., Rahimian, H.A. & Minasian, V. (2010). Plant Pathology. Volume 1, 5th ed. Page 344. (in Farsi)
    18. Jepson, S.B. (1987). Identification of Root-knot nematodes. Cambrian News Ltd.
    19. Krause, G.H. & Weis, E. (1991). Chlorophyll fluorescence and photosynthesis: The basics. Annual Review of Plant Physiology. Plant Molecular Biology, 42, 313-349.
    20. Ma, B.L., Morison, M.J. & Videng, H.D. (1995). Leaf greenness and photosynthetic rates in soybean. Crop Science, 35, 1411-1414.
    21. Maxwell, K. & Johnson, G.N. (2000). Chlorophyll fluorescence- A practical Guide. Experimental Botany, 51, 659-668.
    22. Melakeberhan, H. & Ferris, H. (1989). Impact of Meloidogyne incognita on Physiological Efficiency of Vitis vinifera. Journal of Nematology, 21(1), 74-80P.
    23. Mohsenzadeh, S., Farahi-Ashtiani, C., Mlbobi, M.A. & Ganati, F. (2003). Effect of drought and chlorocholine chloride on seedling growth and photosynthesis of two cultivars of wheat (Triticum aestivum L.). Pajouhesh and Sazandegi, 60, 56-64. (in Farsi)
    24. Netto, A., Campostrini, E., Oliveira, J. & Bressan-Smith, R. (2005). Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 reading in coffee leaves. Scientia Horticulturae, 104, 199-202.
    25. Oostenbrink, M. (1966). Major characteristics of the relationships between nematodes and plants. Meded. Landbouwhoo. Gesch. Wageningen, 66, 1-46.
    26. Ort, D. (2002). Chilling-induced limitations on photosynthesis in warm climate plants: Contrasting mechanisms. Environmental Control in Biology, 40, 7-18.
    27. Oxborough, K. (2004). Imaging of chlorophyll a fluorescence: theoretical and practical aspect of an emerging technigue for the monitoring of photosynthetid performance. Journal of Experimental Botany, 55, 1195-1205.
    28. Perry, R.N., Maurice, M. & Starr, J.L. (2010). Root-Knot Nematodes. CABI Head Office, UK. 531pp.
    29. Rapacz, M., Tokarz, K. & Janowiak, F. (2001). The initiation of elongation growth during long-term low-temperature stay of spring-type oilseed rape may trigger loss of frost resistance and change in photosynthetic apparatus. Plant Science, 161, 231-236.
    30. Rizza, F., Pagani, D., Stance, A.M. & Cattivelli, L. (2001). Use of chlorophyll fluorescence to evaluate the cold acclimation and freezing tolerance of winter and Spring oats. Plant Breeding, 120, 389-396.
    31. Sahebani, N., Zad, J., Sharifiee Tehrani, A. & Kheyri, A. (2006). Study of spore germination, growth rate and orientation of Fusarium oxysporum f.sp. lycopersici toward roots infected by Meloidogyne javanica in vitro. Journal Agriculture Science and Natural Resource, 13(5). Nov-Dec. (in Farsi)
    32. Shaukat, S.S. & Khan, M.A. (2009). Growth and Physiological responses of OKRA (Abelmoschus bsculentus (L.) Moench) to simulated acid rain and Roor-knot Nematode (Meloidogyne incognita). Nematol. Medit. Horticultural Research, 37, 17-23.
    33. Swain, B. & Prasad, S. (1989). Photosynthetic rate in rice as influenced by the Root-knot Nematode, Meloidogyne graminicola, infection. Revue de Nematology, 12(4), 431-432.
    34. Taylor, A.L. & Sasser, J.N. (1978). Biology, identification and control of root-knot nematodes (Meloidogyne spp.). Coop Publisher. Plant. North. Carolina State. University and U.S Agency international Development. Raleigh, N.C. 111pp.
    35. Taylor, D.P. & Netscher, C. (1974). An improved technique for preparing perennial pattern of Meloidogyne spp.. Nematologica, 20, 268.
    36. Ye. De-You, Qianchum- Too & Chen Jin-Feng. (2011). Photosynthetic response to the Root-knot Nematode Meloidogyne incognita in resistant cultivar Sour Cucumber (Cucumis hystria Chaker.) Journal China Agriculture Science, 44(20), 4248-4257P.
    37. Zobayed, S., Afreen, F. & Kozai, T. (2005). Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. Johns Wort. Plant Physiology and Biochemistry, 43, 977-984.

     

Iranian Journal of Plant Protection Science
Volume 46, Issue 2 - Serial Number 2
Autumn & Winter
October 2015
Pages 295-306

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History

  • Receive Date: 12 May 2015
  • Revise Date: 06 December 2015
  • Accept Date: 17 December 2015

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