Iranian Journal of Plant Protection Science

Iranian Journal of Plant Protection Science

Biological control of major fungal causal agent of root and crown rot of bean in Zanjan province with antagonistic bacteria

Document Type : Research Paper

Authors
Afagh Faraji 1
Roghaye Hemmati 2
Alıreza Marefat 3
1 Former M.Sc. Student Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Assistant Professor, Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Razi, Kermanshah, Iran
10.22059/ijpps.2015.57387
Abstract
Root and crown rot of bean caused with Fusarium solani, Rhizoctonia solani and Fusarium oxysporum, is one of the important diseases of bean in Zanjan province. Using native biocontrol agents integrated with chemical agents is one of the effective management strategies. This is also important to choose control methods effective against all major fungal pathogens and mixed contamination of bean, with them. This research was conducted to study synergistic effect of three major fungal pathogens and biocontrol effects of some native rhizobacteria on disease. During the mid-summer of 2011, 46 bacterial isolates were obtained from bean rhizosphere in Zanjan bean fields. Two isolates of each three fungal species (pathogenic on bean) were also received from mycology collection of Zanjan University. Two isolates of Rhizobacteria were chosen as the best biocontrol agents against all three fungi in terms of production of antibiotic, volatile metabolites, HCN and protease and selected for in-vitro antagonistic experiments. Greenhouse results showed that, seed treatment of bean with bacteria, decreased the disease and increased some vegetative factors of the plant. There was synergistic effect among the fungal species.
 
 
 
Keywords
Synergistic effect
Biocontrol
rhizobacteria
fungal agent
Bean

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Iranian Journal of Plant Protection Science
Volume 46, Issue 2 - Serial Number 2
Autumn & Winter
October 2015
Pages 317-329

  • Receive Date 27 December 2014
  • Revise Date 12 July 2015
  • Accept Date 19 October 2015