Lethal and sublethal effects of four insecticides on egg and first larvae green lacewing Chrysoperla carnea in laboratory conditions

Document Type : Research Paper


1 Former M.Sc. Student Aboureyhan Campus, University of Tehran

2 Assistant Professor, Department of Agricultural Entomology, Plant Protection Research Institute, Tehran, Iran

3 Assistant Professor, Aboureyhan Campus, University of Tehran


The common green lacewing is one of the important predators in the field and greenhouse. In this study, lethal effects of azadirachtin, flonicamid, thiacloprid and thiocyclam againstegg and first instar of Chrysoperla carnea were studied in the laboratory. Larval bioassay was carried out with contact method in glass Petri dishes, while egg bioassay was conducted with immersion method. Mortality of eggs and larvae were measured after 72 h and 96 h, respectively. In order to study the sub-lethal effects of the treatments, first instars were subjected to LC25 of each treatment, and the effects were estimated based on IOBC method. Experiments were conducted at 25 ± 2 ºC, 65 ±  5% relative humidity and a photoperiod of 16: 8 h (L:D). The estimated LC50 of azadirachtin, flonicamid, thiacloprid and thiocyclam  for eggs were 510, 451, 394, 862 ppm, while for the first instar, it was 596, 37, 124  271 ppm, respectively. The mean fertility rate for control, azadirachtin, flonicamid, thiacloprid and thiocyclam were 291.32±13.7, 279.67±11.86, 235.75±12.84, 177.84 ± 13.87 and 99.85 ± 10.58 eggs, respectively. Based on IOBC classification method, azadirachtin and flonicamid were categorized as 'not harmful', while thiacloprid and thiocyclam were categorized as 'slightly harmful' against C. carnea. If our results are corroborated with field tests, flonicamid and azadirachtin can be incorporated in integrated pest managements of the field where this predator is active.



    1. Ako, M., Borgemeister, G., Poehling, H. M., Elbert, A. & Nauen, R. (2004). Effect of neonicotinoid insecticides on bionomics of two spotted spider mite (Acari: Tetranychidae). Journal of Economic of Entomology, 97, 1587-1594.
    2. Ayubi, A., Moravvej, Gh., Karimi, J. & Jooyandeh, A. (2013). Lethal effects of four insecticides on immature stages of Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) in laboratory conditions. Turkish Journal of Entomology, 37, 399-407
    3. Bailey, W. J. (1991). Acoustic Behavior of insects: an evolutionary perspective. Chapman & Hall, New York. 356 pp
    4. Bortolotti, L., Micciiarelli Sbrenna, A. & Sbrenna, G. (2005). Action of fenoxycarb on metamorphosis cocoon spinning in Chrysoperla cornea (Neuroptra: Chrysopidae): identification of the JHA-sensitive period. Eurropean journal of Entomology, 102, 27-32.
    5. Bueno, A.F. & Freitas, S. (2004). Effects of the insecticides abamectin and lufenuron on eggs and larvae of chrysoperla externa under laboratory condition. Biological Control, 49, 277-283.
    6. Canard, M., Semeria, Y. & New, T. R. (1984). Biology of cherysopidae. W. Junk Publishers. 308pp.
    7. Croft, B. A. (1990). Arthropod biological control agents and pesticides. John Wiley, New York, 723 pp.
    8. Desneux, N., Decourtye, A. & Delpuech, J. M. (2007). The sublethal effects of pesticides on beneficial arthropods. Annual Review of Entomology, 52, 81-106.
    9. Golmohammadi, Gh., Hejazi, M., Iranipour, Sh. & Mohammadi, S. A. (2009). Lethal and sublethal effects of endosulfan, imidacloprid and indoxacarb on first instars larvae of Chrysoperla carnea (Neuroptera: Chrysopidae) in laboratory. Journal of Entomological Society of Iran, 28, 35-47.
    10. Golmohammadi, Gh., Hejazi, M., Iranipour, Sh. & Mohammadi, S.A. (2012). Effects of imidacloprid, indoxacarb and endosulfan on egg, third-instar larva and pupa of green lacewing Chrysoperla carnea (Neu.: Chrysopidae). Journal of Entomological Society of Iran, 31, 37-50
    11. Golmohammadi, Gh. & Hejazi, M. (2014). Toxicity and side effects of three insecticides on adult Chrysoperla carnea (Neu.:Chrysopidae) under laboratory conditions. Journal of Entomology Society of Iran, 33, 23-28.
    12. Hassan, S.A. (1994). Comparison of three laboratory methods and one semi-field test method to assess side effects of pesticides on Trichogramma cacoeciae. Bulletin OILB SROP, 17, 133-141. 
    13. Kazemi, F. (2012). The side effect of insecticides Envidore, Actara, Mospilan, Calipso and Mitac on common green lacewings (Chrysoperla carnea) and their effect on development and fecundity of this insect in laboratory condition. from: agrisis.areo.ir/_agrisis/documents/42030.pdf
    14. Mandour, N. S. (2009). Influence of spinosad on immature and adult stages of Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae). BioControl, 54, 93-102.
    15. Medina, P., Budia, F., Tirry, L., Smagghe, G. & Viñuela, E.  (2001). Compatibility of spinosad, tebufenozide and azadirachtin with eggs and pupae of the predator Chrysoperla carnea (Stephens) under laboratory conditions. Biocontrol Science and Technology, 11, 597-610.
    16. Medina, P., Budia, F., Estal, P. D., Adan, A. & Viñeula, E. (2003). Side effects of six insecticides on different developmental stages of Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae). Pesticides Beneficial Organization IOBC/wprs Bulletin, 26, 33-40.
    17. Moens, J., Gent, U., Patrick, D. C. & Gent, L. T. (2011). Side effects of pesticides on the larvae of the hoverfly Episyrphus balteatus in the laboratory. Phytoparasitica, 39, 1-9.
    18. Nasreen, A., Ashfaq, M., Mustafa, G. & Rasoolkhan, R. (2005). Mortality of Chrysoperla carnea (Stephens), (Neuroptera: Chrysopidae) after exposure to some insecticides; laboratory studies. Journal of South Pacific studies, 26, 1-6.
    19. Rezaei, M., Talebi, K., Naveh, V. H. & Kavousi, A. (2006). Impacts of the pesticides imidacloprid, propargite, and pymetrozine on Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae): IOBC and life table. BioControl, 52, 385-398.
    20. Robertson, J. L. & Preisler, H. K. (1992). Pesticides bioassays with arthropods, CRC Press, 127 pp.
    21. Saber, M., Hejazi, M. J., Kamali, K. & Moharramipour, S. (2005). Lethal and sublethal effects of fenitrothion and deltamethrin residues on the egg parasitoid Trissolcus grandis (Hymenoptera: Scelionidae). Journal of Economic Entomology, 98, 35-40.
    22. Stapel, J. O., Cortesero, A. M. & Lewis, W. J. (2000). Disruptive sublethal effects of insecticides on biological control: Altered foraging ability and life span of the parasitoid after feeding on extrafloral nectar of cotton treated with systemic insecticides. Biological Control, 17, 243-249.
    23. Vogt, H. (1994). Effects of pesticides on Chrysoperla carnea in the field and comparison with laboratory and semi-field results. Pesticides Beneficial Organization IOBC/wprs Bulletin, 17, 71-82.