The toxicity of acetamiprid, dicholorvos and azadirachtin pesticides on melon aphid, Aphis gossypii Glover and Lysiphlebus fabarum Marshall

Document Type : Research Paper

Authors

1 Department of Plant Protection, College of Agricultural, Shahid Chamran University, Ahvaz, Iran

2 Department of Plant Protection, Agricultural and Natural Resources Research Center of Hormozgan, Bandar Abbas, Iran

3 Department of Plant Protection, Agricultural and Natural Resources Research Center of Khozestan, Ahvaz, Iran

Abstract

Cotton aphid, Aphis frangulae gossypii Glover, is a major pest of cucumber in Iran. One of the important parasitoids playing role in population control of this pest in the fields and greenhouses is Lysiphlebus fabarum Marshall. In this study, we investigated sensitivity of different instars of cotton aphids and pupal stage of L. fabarum to field recommended and half-recommended concentration of three pesticides, acetamiprid, dichlorvos and azadirachtin by leaf dipping method. The persistence toxicity test and bioassays were also carried out on adult of parasitoid. Results revealed a significant reduction in the toxicity by increasing the nymphal stages of the aphid. These results also demonstrated the highest mortality for dichlorvos on pupal stage ofparasitoid. According to obtained results, LC50 of acetamiprid was 28.18, for dichlorvos was 7.052 and for azadirachtin was 1780.338, µg/lit on adult of parasitoid respectively. Based on IOBC categories, azadirachtin with stability of less than 5days, dichlorvos with stability 5-15 days and acetamiprid with stability between 15-31 days were classified as short lived (class A), slightly persistent (class B) and moderately persistent (class C), respectively. These results approved a better integration potential of azadirachtin with the parasitoid, L. fabarum, for aphid control.

Keywords


  1. Amini Jam, N., Kocheyli, F., Mossadegh, M.S., Rasekh, A. & Saber, M. (2014). Lethal and sublethal effects of imidacloprid and pirimicarb on the melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae) under laboratory conditions. Journal of Crop Protection, 3(1), 89-98.
  2. Ashoka, J., Bheemanna, M., Nagangouda, A., Sreenivas, A. G. & Mekali, J. (2013). Waiting period for insecticides and a botanical used in control of Mulberry Thrips. Annals of Plant Protection Sciences, 21(1), 42-45.
  3. Attia, A. A. & El-Hamaky, M. A. (1987). The biology of the cotton aphid Aphis gossypii Glover in Egypt (Homoptera: aphididae). Bulletin Societe Entomologique Egypte, 85, 359-371.
  4. Baghery-Matin, Sh., Sahragard, A. & Rasoolian, G. (2005). Some behavioural characteristics of Lysiphlebus fabarum (Hymenoptera: Aphidiidae) parasiting Aphis fabae (Homoptera: Aphididae) under laboratory conditions. Journal of Entomology, 20, 64-68.
  5. Baroon, N. (2007). A study of population fluctuations of black bean aphid, Aphis fabae (Homoptera: Aphididae) and the efficiency of its parasitoid Lysiphlebus fabarum Marshall (Hymenoptera: Braconidae) on Faba bean in Ahvaz. M. Sc. dissertation. Shahid Chamran University of Ahvaz. (in Farsi)
  6. Blackman, R.L. & Eastop, V.F. (1984). Aphids on the world's crops. An identification and information guide. John Wiley and Sons Ltd., Chichester, U k.
  7. Busvin, J. R. (1971). A critical review of the techniques for testing insecticides: Common Wealth Agricultural Bureau, London, 345 pp. 
  8. Chang-Geun, Y. i., Byeoung-Ryeol, Ch., Hyung-Man, P., Chang-Gyu, P. & Young-Joon, A. (2006). Fumigant toxicity of plant essential oils to Thrips palmi (Thysanoptera: Thripidae) and Orius strigicollis (Heteroptera: Anthocoridae). Journal of Economic Entomology, 99, 1733-1738.
  9. Debach, P. & Rosen, D. (1991). Biological control by natural enemies. Cambrige Universuty Press.
  10. Desneux, N., Decourtye, A. & Delpuech, J. M. (2007). The sublethal effects of pesticides on beneficial arthropods. Annual Review of Entomology, 52, 81-106.
  11. Isman, M.B. (2006). Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated World. Annual Review of Entomology, 51(1), 45-66.
  12. Kavallieratos, N.G., Tomanovic, Z., Stary, P., Athanassiou, C.G., Sarlis, G.P., Petrovic, O., Niketic, M. & Anagnou-Veroniki, M. (2004). A survey of aphid parasitoids (Hymenoptera: Braconidae: Aphidiinae) of southeastern Europe and theiraphid-plant associations. Applied Entomology and Zoology, 39, 527-563.
  13. Kontsedalov, S., Gottlieb, Y., Ishaaya, I., Nauen, R., Horowitz, R. & Ghanim, M. (2009). Toxicity of spiromesifen to the developmental stages of Bemisia tabaci biotype B. Pest Management Science, 65(1), 5-13. 
  14. Kresting, U., Satar, S. & Uygun, N. (1999). Effect of temperature on development rate and fecundity of apterous Aphis gossypii Glover (Homoptera: Aphididae) reared on Gossypium hirsutum. Journal of Applied Entomology, 123(1), 23-27.
  15. LeOra Software. (2006). POLO-Plus 1.0 Probit and Logit Analysis. LeOra Software, Petaluma. 
  16. Liu, B. & Sengonca, C. (2002). Investigations on side‐effects of the mixed biocide GCSC‐BtA on different predators of Plutella xylostella Linnaeus (Lepidoptera: Plutellidae) in southeastern China. Anzeiger für Schädlingskunde, 75(3), 57-61.
  17. Martinez-Villar, E., Saenz-de-Cabezon, F.J., Moreno-Grijaba, F., Marco, V. & Perez-Moreno, I. (2005). Effects of Azadirachtin on the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Experimental and Applied Acarology, 35(3), 215-222.
  18. Millar, N.S. & Denholm, I. (2007). Nicotinic acetylcholine receptors: targets for commercially important insecticides. Invertebrate Neuroscience, 7(1), 53-66.
  19. Moens, J., Tirry, L. & Clercq, P. (2012). Susceptibility of cocooned pupae and adults of the parasitoid Microplitis mediator to selected insecticides. Phytoparasitica, 40, 5-9.
  20. Mordue, A.J. & Blackwell, A. (1993). Azadirachtin: an update. Journal of Insect Physiology, 39, 903-924.
  21. Noorbakhsh, S. & Sahraeian, H. (2015). List of pests, diseases and weeds important agricultural products; Pesticides and recommended practices to control them. Prognosis Bureau of Plant Protection Organization, 208 pp. (in Farsi)
  22. Polgar, L. & Sagi, K. (1983). The effect of pesticides on a beneficial hymenopterous parasite: Aphidius matricariae Haliday. Bulletin of International Conference on Integrated Plant Protection, Budapest, 4, 65-69.
  23. Prabhaker, N., Toscano, N.C. & Coudriet, D.L. (1989). Susceptibility of the immature and adult stage of the sweet-potato whitefly to selected insecticides. Journal of Economic Entomology, 82(4), 938-988. 
  24. Rezaei, N. (2014). Effects of two insecticides on biological parameters of mustard aphid Lipaphis erysimi kal and its parasitoid Diaeretiella rapae M'Intosh and the effects of these insecticides on the aphid's energy metabolism. Ph.D. Thesis. Shahid Chamran University of Ahvaz. (in Farsi)
  25. Rouhani, M., Samih, M. A., Izadi, H. & Mohmmadi, E. (2013). Toxicity of new insecticides against pomegranate aphid, Aphis punicae Passerini. International Research Journal of Applied and Basic Sciences, 4(3), 496-501.
  26. Sabahi, Q., Kosari, A. & Ashore, A. (2010). Side effects of four pesticides imidacloprid, dichlorvos, pymethrozine and abamectin on Orius albidipennis Reuter (Hemiptera: Anthocoridae). Journal of Plant Protection Science, 40(1), 9-16. (in Farsi)
  27. Sabahi, Q., Rasekh, A. & Michaud, J.P. (2011). Toxicity of three insecticides to Lysiphlebus fabarum, a parasitoid of the black bean aphid Ahis fabae. Journal of Insect Science, 11, 1-8.
  28. Schuld, M. & Schmuck, R. (2000). Effects of thiacloprid, a new chloronicotinyl insecticide, on the egg parasitoid Trichogramma cacoeciae. Ecotoxicology, 9, 197-205.
  29. Stark, J. D. & Rangus, T. (1994). Lethal and sublethal effects of the neem insecticide, Margosan-O, on the pea aphid. Pesticide Science, 41, 155-60.
  30. Stark, J. D. & Wennergren, U. (1995). Can population effects of pesticides be predicted from demographic toxicological studies? Journal of Economic Entomology, 88(5), 1089-1096. 
  31. Stary, P. (1999). Aggregations of aphid parasitoid adults (Hymenoptera, Aphidiidae). Journal of Applied Entomology, 105, 270-279.
  32. Tadeo, L. (2008). Analysis of pesticides in food and environmental samples CRC Press, 382 pp.
  33. Tang, Y. Q., Weathersbee, A. A. & Mayer, R. T. (2002). Effect of neem seed extract on the brown citrus aphid (Homoptera: Aphididae) and its parasitoid Lysiphlebus testaceipes (Hymenoptera: Braconidae). Environmental Entomology, 31(1), 172-176.
  34. Ukeh, D.A., Emosairie, S.O., Udo, I.A. & Ofem, U.A. (2007). Field Evaluation of Neem, (Azadirachta indica A. Juss) Products for the management of lepidopterous stem borers of maize, (Zea mays) in Calabar, Nigeria. Research Journal of Applied Sciences, 2(6), 653-658.
  35. Wang, X.Y., Yang, Z.Q., Shen, Z.R., Lu, J. & Xu, W.B. (2008). Sublethal effects of selected insecticides on fecundity and wing dimorphism of green peach aphid (Homoptera: Aphididae). Journal of Applied Entomology, 132(2), 135-142.