Iranian Journal of Plant Protection Science

Iranian Journal of Plant Protection Science

Molecular characterization of a Potato virus Y isolate from tomato crop in Yazd (Iran) based on sequencing of partial genome

Document Type : Research Paper

Authors
Seyed reza Mirrahimi Bidakhavidi 1
Samin Hosseini 2
Ahmad Hosseini 2
Seyyed Alireza Esmailzadeh Hosseini 3
1 Former M. Sc. Student, Department of Plant Protection, College of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
2 Assistant Professor, Department of Plant Protection, College of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
3 Instructor, Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran
10.22059/ijpps.2017.140753.1006685
Abstract
Tomato (Lycopersicum esculentum Mill.) is one of the most important crops in Iran and throughout the world. In the previous survey conducted in 2013, PVY was introduced as the dominant virus in Yazd (Iran). In the present study, the partial genome sequence of a selected isolate called T5 was determined and analyzed. Following total RNA extraction of the T5 isolates, three fragments with 824, 1700 and 837 bp lengths were amplified using CPr/CPf, M4T/Sprimer and P1/P2 primer pairs respectively in RT-PCR. According to the nucleotide and amino acid sequences comparison of the CP, NIb and P1 regions of the isolate with the other PVY isolates in the Genebank, T5 is a member of NTN strain.
Keywords
Coat protein
P1 gene
RT-PCR
PVY
Tomato
  1. Anonymous. (2014). Agricultural statistics, Ministry of Agriculture Jihad, 169p, from http://www.maj.ir/. (in Farsi)
  2. Barret, A. J. (1986). An introduction to the proteinases; in Proteinase inhibitors, New York, Elsevier.
  3. Chen, J. & Adams, M. J. (2001). A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Archives of Virology, 146, 757-766.
  4. Clark, M. F. & Adams, A. M. (1977). Characteristics of the microplate method of enzyme-linked Immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475-483.
  5. Chung, B. Y. W., Miller, W. A., Atkins, J. F. & Firth, A. E. (2008). An overlapping essential gene in the Potyviridae. Proceedings of the National Academy of Sciences, 105, 5897-5902.
  6. Danci, O., Ziegler, A., Torrance, L., Gasemi, S. & Danci, M. (2009). Potyviridae family-short review. Journal of Horticulture, Forestry and Biotechnology, 13, 410-420.
  7. De Bokx, J. & Huttinga, H. (1981). Potato virus Y. CMI/AAB Descriptions of Plant Viruses. N. 242.
  8. Domier, L. L., Franklin, K. M., Shahabuddin, M., Hellmann, G. M., Overmeyer, J. H., Hiremath, S. T., Siaw, M. F., Lomonossoff, G. P., Shaw, J. G. & Rhoads, R. E. (1986). The nucleotide sequence of Tobacco vein mottling virus RNA. Nucleic Acids Research,14, 5417-5430.
  9. Folwarczna, J., Plchova, H., Moravec, T., Hoffmeisterova, H., Dedic, N. & Cerovska, N. (2008). Production of polyclonal antibodies to a recombinant coat protein of Potato virus Y. Folia Microbiologica, 53 (5), 438-442.
  10. Hema, M., Sreenivasulu, P. and Savithri, H. (2002). Taxonomic position of Sugarcane streak mosaic virus in the family Potyviridae. Archives of Virology, 147, 1997-2007.
  11. Hosseini, A., Massumi, J., Heydarnejad, J., Hosseinipour, A. & varsani, A. (2010). Characterisation of Potato virus Y isolates from Iran.Virus Genes, 42, 128-140.
  12. Jafari, M., Valizadeh, M., Valizadeh, G., Artyay, F. & Bigami, M. (2010). Detection of important viruses insecting cucurbits and tomato in fields and greenhouses of baluchstan. In: Proceeding of 19th Iranian plant protection congress, 1-3 Aug, iranian Research Institute of Plant Protection, Tehran, Iran, pp. 669. (in Farsi)
  13. Jones, R., Kumar, S. & Mackie, A. (2003). Potato virus Y. Western Australia Department of Agriculture, 1443-7783.
  14. Khakvar, R., Pourahim, R. & Shamsbakhsh, M. (2000). Occurrence of C strain potato virus Y in Khuzestan province. In: Proceeding of 14th Iranian plant protection congress, 5-8 Sep., Isfahan University of Technology, Isfahan, Iran ,pp. 108-109. (in Farsi)
  15. Koonin, E. V. & Dolja, V. V. (1993). Evolution and taxonomy of positive- strand RNA viruses: Implications of comparative analysis of amino acid sequences. Critical Reviews in Biochemical and Molecular Biology, 28, 375-430.
  16. Maghsoudi, R., Jafarpour, B. & Falahaty, M. (2004). Occurrence of N strain potato virus Y in Khorasan province and O strain Potato Virus Y in Khorasan and Gilan and Mazandaran Provinces. In: Proceeding of 19th Iranian Plant Protection Congress. 28-30 Aug., Tabriz University, Tabriz, Iran, pp. 223-224. (in Farsi)
  17. Masoumi, H., Heidarnejad, G. & Hosseinipoor, A. (2008). Evaluation of tomato varieties to natural infection with farms and greenhouses viruses in central and south east area in Iran. Quarterly of Agricultural Sciences and Natural Resources, 16(1), 467-473. (in Farsi)
  18. Mirrahimi, S. R., Hosseini, S., Hosseini, A., Esmailzadeh Hosseini, S. A. & Mohammadi, E. (2015). Occurrence and distribution of mosaic inducing viruses and detection of pvy strains from tomato fields and greenhouses of yazd. Journal of Plant Protection, 29(1), 154-158. (in Farsi)
  19. Missiou, A., Kalantidis, K., Boutla, A., Tzortzakaki, S., Tabler, M. & Tsagris, M. (2004). Generation of transgenic potato plants highly resistant to Potato virus Y (PVY) through RNA silencing. Molecular Breeding,14, 185-197.
  20. Shukla, D. D., Ward, C. W. & Brunt, A. A. (1994). The Potyviridae. Cab International.
  21. Singh, R. P., Valkonen, J. P., Gary, S. M., Boonham, N., Jones, R. A., Kerlan, C. & Schubert, J. (2008). Discussion paper: The naming of Potato virus Y strain infecting potato. Archives of Virology, 153, 1-13.
  22. Toosi, N., Ahunmanesh, A., Purrahim, R. & Bahar, M. (2004). Identification C and V Strains of Potato Virus Y using RT-PCR and RFLP. In: proceeding of 14th Iranian Plant Protection Congress. 28-30 Aug., Tabriz University, Tabriz, Iran, pp. 224-225. (in Farsi)
  23. Urcuqui-Inchima, S., Haenni, A. L. & Bernardi, F. (2001). Potyvirus proteins: a wealth of functions. Virus Research, 74, 157.
  24. Visser, J. C. & Bellstedt, D. U. (2009). An assessment of molecular variability and recombination patterns in South African isolates of Potato virus Y. Archives of Virology, 154, 1891-1900.
Iranian Journal of Plant Protection Science
Volume 47, Issue 2 - Serial Number 2
December 2016
Pages 189-199

  • Receive Date 31 January 2016
  • Revise Date 03 October 2016
  • Accept Date 19 October 2016