Molecular characterization of a Potato virus Y isolate from tomato crop in Yazd (Iran) based on sequencing of partial genome

Document Type : Research Paper

Authors

1 Former M. Sc. Student, Department of Plant Protection, College of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran

2 Assistant Professor, Department of Plant Protection, College of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran

3 Instructor, Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran

Abstract

Tomato (Lycopersicum esculentum Mill.) is one of the most important crops in Iran and throughout the world. In the previous survey conducted in 2013, PVY was introduced as the dominant virus in Yazd (Iran). In the present study, the partial genome sequence of a selected isolate called T5 was determined and analyzed. Following total RNA extraction of the T5 isolates, three fragments with 824, 1700 and 837 bp lengths were amplified using CPr/CPf, M4T/Sprimer and P1/P2 primer pairs respectively in RT-PCR. According to the nucleotide and amino acid sequences comparison of the CP, NIb and P1 regions of the isolate with the other PVY isolates in the Genebank, T5 is a member of NTN strain.

Keywords

References

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Iranian Journal of Plant Protection Science
Volume 47, Issue 2 - Serial Number 2
December 2016
Pages 189-199

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History

  • Receive Date: 31 January 2016
  • Revise Date: 03 October 2016
  • Accept Date: 19 October 2016

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