Investigation of biological species concept in some Armillaria species in Iran

Document Type : Research Paper

Authors

1 Ph. D. Candidate, Department of Plant Protection, Faculty of Agricultural Science and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professr, Department of Plant Protection, Faculty of Agricultural Science and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Professor, Department of Plant Protection, Faculty of Agricultural Science and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

4 Assistant Professor, Department of Botany, Iranian Plant Protection Research Institute, Tehran, Iran

Abstract

During September to November 2010-2012, 17 fruiting body specimens of Armillaria were collected from diverse forest sites in Guilan, Mazandaran and Golestan Provinces (Iran) and 24 basidiocarp derived isolates were obtained. Mating tests were performed using 10 Iranian representative isolates and 12 European tester strains of European species including A. mellea, A. gallica and A. cepistipes. Crossing tests were also used for identification of interspecific and intraspecific separation criteria. In the mating test of a total number of 360 interspecific crosses, Iranian isolates were divided into two A. mellea and A. gallica groups. Hemi-compatible and compatible reactions were found in 126 interspecific crosses. Analysis of crosses demonstrated clear separation for both MFF (mycelial fusion frequency) and BLF (black line frequency) and also change in culture morphology. Mating data clearly distinguished three species including A. mellea, A. gallica and A. cepistipes and defined sexual compatibility of Iranian species with their European counterparts. In spite of close phylogenetic relationship between Iranian A. gallica and A. cepistipes, it also represented a significant clear cut based on the sexual fertility. The results suggest that biological species concept in these fungi consist of using both somatic and sexual compatibilities that still can be used in case of selection of a proper pattern of isolates.

Keywords

References

  1.  

    1. Anderson, J. B. & Ullrich, R. C. (1979). Biological species of Armillaria mellea in North America. Mycologia, 71, 402-14.

    2. Antonin, V., Tomsovsky, M., Sedlak, P., Majek, T. & Jankovsky, L. (2009). Morphological and molecular characterization of the Armillariacepistipes-gallica complex in the Czech Republic and Slovakia. Mycological Progress, 8, 259-271.

    3. Asef, M. R., Mohammadi Goltapeh, E. & Alizadeh, A. (2003). Identification of Armillaria biological species in Iran. Fungal Diversity, 14, 51-60.

    4. Banik, M. T. (1996). Armillarianabsnona, a new species from western North America. Mycologia, 88, 484-491.

    5. Banik, M. T. & Burdsall, H. H. (1998). Assessment of compatibility among Armillaria cepistipes, A. sinapina, and North American Biological species X and XI using culture morphology and molecular biology. Mycologia, 90, 798-805.

    6. Berubee, J. A., Dessureault, M., Betthelay, S. & Guillaumin, J. J. (1996). Interfertility between Armillaria cepistipes and A. sinapina. Phytoprotection, 77, 67-74.

    7. Brazee, N. J., Helvey, J. P. & Wick, R. L. (2011). Evaluation of partial tef1, rpb2, and nLSU sequences for identification of isolates representing Armillaria calvescens and Armillaria gallica from northeastern North America. Fungal Biology, 115, 741-749.

    8. Coetzee, M. P. A., Wingfield, B. D. & Zhao, J., Van Coller, S. J., Wingfield, M. J. (2015). Phylogenetic relatioship among biological species of Armillaria from China. Mycoscience, 56(5), 530-541.

    9. Davari, M. & Askari, B. (2005). Armillaria mellea as a cause of oak decline in Hatam-baigh forest of Iran. Communications in Agricultural and Applied Biological Sciences, 70(3), 295-304.

    10. Dumas, M. T. (1998). Biological species of Armillaria in the mixed wood forest of northern Ontorio Canada. Canadian Journal of Forest Research, 18, 872-874.

    11. Ferguson, B. A., Dreisbach, T. A., Parks, C. G., Filip, G. M. & Schmitt, C. L. (2003). Coarse-scale population structure of pathogenic Armillaria species in a mixed-conifer forest in the Blue Mountains of northeast Oregon. Canadian Journal of Forest Research, 33, 612-623.

    12. Fox, R. T. V. (2000). Pathogenicity, Chapter 6. pp. 113-136 in Fox, R. T. V., ed. Armillaria Root Rot: Biology and Control of Honey Fungus. Intercept Limited, Andover, UK. 222 p.

    13. Hasegawa, H., Ota, Y. & Kikuchi, T. (2010). Sequence based identification of Japanes Armillaria species using the elongation factor-1 alpha. Mycologia, 102(4), 895-910.

    14. Hintikka, V. (1973). A note on the polarity of Armillariella mellea. Karstenia, 13, 32-39.

    15. Kile, G. A. & Watling, R. (1998). Identification and occurrence of Australian Armillaria species including A. pallidula sp. nov.and comparative studies between them and non- Australian tropical and Indian Armillaria. Transaction of the British Mycological Society, 91, 305-315.

    16. Kim, M. S. & Klopfenstein, N. B., Hanna, J. W., McDonald, G. I. (2006). Characterization of North American Armillaria species: genetic relationships determined by ribosomal DNA sequences and AFLP markers. Forest Pathology, 36, 145-164.

    17. Kim, M-S., Klopfenstein, N. B., McDonald, G. R., Arumuganathan, K. & Vidaver, A. K. (2001). Use of flowcytometry, flouresence microscopy and PCR-based techniques to assessintras pecific and interspecific matings of Armillaria species. Mycologica Research, 105, 153-163.

    18. Korhonen, K. (1978). Interfertility and clonal size in the Armillariella mellea complex. Karstenia, 18, 31-42.

    19. Korhonen, K. (1995). Armillaria since Elias Fries. Acta Univ.symb. Bot. Ups. (pp.: 153-161).

    20. Morrison, D. J., Chu, D. & Johnson, A. L. S. (1985). Species of Armillaria in British Columbia. Canadian Journal of Plant Pathology, 7(3), 242-246.

    21. Ota, Y., Matsushita, N., Nagasawa, E., Terashita, T., Fukuda, K. & Suzuki, K. (1998). Biological species of Armillaria in Japan. Plant Disease, 82, 537-548.

    22. Ota, Y., Sotome, K. & Hasegawa, E. (2009). Seven Armillaria species identified from Hokaido Island, Northern Japan. Mycoscience, 50, 442-447.

    23. Peterson, R. H. (1995). There is more to a mushroom than meets the eye: mating studies in the Agaricales. Mycologia, 87, 1-17

    24. Qin, G. F., Zhao, J. & Korhonen, K. (2007). A study on intersterility groups of Armillaria in china. Mycologia, 99(3), 430-441.

    25. Shaw, C. & Klie, A. (1991). Armillaria root disease. Agricultural Hand book. No.691, USDA Forest Service, Washington, USA.

    26. Smith, M. L., Bruhn, J. N. & Anderson, J. B. (1992). The fungus Armillaria bulbosa is among the largest and oldest living organisms. Nature, 356, 428-431.

    27. Vilgalys, R. (1991). Speciation and species concept in the Collybiadryophila complex. Mycologia, 83, 758-773.

    28. Worall, J. J. (1991). Media for selective isolation of Hymenomycetes. Mycologia, 83(3), 296-302.

    29. Worall, J. J. (1997). Somatic incompatibility in basidiomicetes. Mycologia, 89, 24-36

Iranian Journal of Plant Protection Science
Volume 47, Issue 2 - Serial Number 2
December 2016
Pages 241-252

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History

  • Receive Date: 08 February 2016
  • Revise Date: 22 August 2016
  • Accept Date: 22 November 2016

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