Identification and partial characterization of the virus infecting saffron (Crocus sativus) in Iran

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Professor, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

4 Associate Professor, Department of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

5 Former M.Sc. Student, Department of Horticultural Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

Following the random sampling of saffron (Crocus sativus L.)leaves and corms from six provinces of Iran, during growing seasons of 2011-2015, a total of 890 leaf tissue samples were investigated by enzyme-linked immunosorbent assay (ELISA) tests. The results revealed that 641 samples showed positive reaction only with Potyvirus genus specific and exactly with Bean common mosaic virus (BCMV) specific antibodies. The molecular mass of several isolates of the virus coat protein (CP) was estimated as 35 kDa by western blotting. Following total RNA extraction from virus infected samples, three discontinuous fragments (corresponding to genomic 3', partial CI and HC-Pro regions) were amplified. The CP region of the virus infecting saffron has the highest nucleotide identity with Ceratobium mosaic virus (CerMV) and Telosma mosaic virus (TelMV) (74%) and the highest deduced amino acid identity with Bean common mosaic necrosis virus (BCMNV) (78.8%). Despite the high serological relationship with BCMV and a close phylogenetic relationship with BCMV subgroup based on CP region, the identity values are less than described species demarcation criteria for Potyviridae family. Overall, it seems that the saffron infecting Potyvirus is a new species of Potyvirus genus but the definitive statement requires sequencing the entire genome of the virus.

Keywords

References

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Iranian Journal of Plant Protection Science
Volume 47, Issue 2 - Serial Number 2
December 2016
Pages 263-275

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History

  • Receive Date: 18 September 2016
  • Revise Date: 01 December 2016
  • Accept Date: 13 December 2016

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