Inducing systemic resistance of tomato by salicylic acid and two biocontrol agents against root- knot nematode

Document Type : Research Paper

Authors

1 Former M.Sc. Student, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Assistant Professor, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Assistant Professor, Faculty of Agriculture, Shahed University, P.O. Box: 33191-18651, Tehran, Iran

4 Instructor, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

To evaluate the effects of the fungus Trichoderma viride, the bacterium Pseudomonas fleurescens CHA0 and salicylic acid against root-knot nematode (Meloidogyne incognita race 2) on the defense enzyme production process of tomato, a pot experiment was conducted under greenhouse conditions. The nematode populations were reproduced on tomato Rutgers cultivar and the test plants were inoculated at four-leaf stage. The peroxidase, phenylalanine ammonia lyase and catalase activities were measured in the first, fourth and seventh days after nematode inoculation. The results showed that all three application modes of fungi, bacteria, and salicylic acid, increased nematode control and significant decreases occurred in the number of gall and egg mass. The infected plants inoculation with fungi, bacteria and salicylic acid, in the cultivars Gina VF, Falat CH, Falat 111 and Karoon reduced the percentage of gall (81, 68, 80, and 83), the number of egg mass (87, 78, 83, and 88) and reproductive factors (83, 69, 82, and 84), respectively. The highest numbers of galls were observed in Karoon, Flat 111, Gina VF and Flat CH in the absence of control agents, respectively. The three agents not only reduced the amount of disease but also increased the activity of catalase peroxidase, and phenylalanine ammonia lyase enzymes in plants, respectively. The enzymes activity reached a maximum on the 4th day after inoculation.

Keywords

References

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Iranian Journal of Plant Protection Science
Volume 48, Issue 2 - Serial Number 2
February 2018
Pages 283-294

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History

  • Receive Date: 21 January 2017
  • Revise Date: 22 July 2017
  • Accept Date: 11 September 2017

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