Identification of mating type idiomorphs in Paecilomyces formosus, the causal agent of die-back disease in pistachio and some other hosts and investigation on the possibility of in vitro sexual reproduction

Document Type : Research Paper

Authors

1 Ph. D. Candidate, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Assistant Professor, Horticultural Science Research Institute, Pistachio Research Center, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan, Iran

4 Professor, Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

Pistachio die-back disease is an important disease in Iranian pistachio orchards. One of the most important fungal agents of the disease is Paecilomyces which has been recently identified as P. formosus based on phylogenetic and physiologic studies. In this study, four pairs of primers designed using mating type idiomorph sequences of P. variotii deposited in GenBank (NCBI). Designed primers were tested in some isolates of P. formosus and finally two primer pairs including; Mat1-1f224 and Mat1-1r224 for amplification of Mat1-1 and Mat1-2f165 and Mat1-2r165 primers for amplification of Mat1-2 idiomorphs were selected. Mating type idiomorphs were amplified in 124 isolates of P. formosus which have been obtained from pistachio trees and eight other species including; Pistacia mutica, Punica granatum, Prunus amygdalus, Caesalpinia gilliesii, Nerium oleander, Tamarix aphylla, Tamarix hispida, Haloxylon sp. and air of pistachio orchards. In 50 isolates (40.3%) Mat1-1, in 59 isolates (47.6%) Mat1-2 and in 15 isolates (12.1%) both idiomorphs were identified. 16 isolates from each mating type and three isolates which had both idiomorphs were selected and crossed in all of the possible combinations on PDA culture medium and cultures were incubated at 25 °C in continuous dark condition. After eight months, sexual reproduction was not observed in crosses.

Keywords

References

  1. Alizadeh, A., Alaei, H. & Ershad, D. (2000). Etiological study on dieback disease of pistachio trees in Rafsanjan. Journal of Modares Agricultural Sciences, 1(2), 53-63. (in Farsi)
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410.
  3. Aminaei, M. M. & Ershad, D. (1989). Pistachio dieback disease in Kerman province. In: Proceedings of 9th Iranian Plant Protection Congress, 8-13 Sept., Mashhad, Iran. P. 82.
  4. Ashkan, M., Abusaidi, D. & Ershad, D. (1997). Etiological study of dieback and canker of pistachio nut tree in Rafsanjan. Iran. Iranian Journal of Plant Pathology, 33, 15-26. (In Farsi) & 5-6. (in English)
  5. Blastn [online software]. (2014). Retrieved July 30, 2014, from https://blast.ncbi.nlm.nih.gov
  6. Chromas Pro 1.7.6 [computer software]. (2014). Retrieved July 30, 2014, from http://technelysium.com.au/wp/chromaspro.
  7. Burdon, J. J. & Thrall, P. H. (2009). Co-evolution of plants and their pathogens in natural habitats. Science, 324, 755-756.
  8. Coppin, E., Debuchy, R., Arnaise, S. & Picard, M. (1997). Mating types and sexual development in filamentous Ascomycetes. Microbiology and Molecular Biology Reviews, 61(4), 411-428.
  9. Davis, R. H. & Weller, S. G. (1998). The gist of genetics. Jones and Bartlett publishers.
  10. Döll, K., Chatterjee, S., Scheu, S., Karlovsky, P. & Rohlfs, M. (2013). Fungal metabolic plasticity and sexual development mediate induced resistance to arthropod fungivory. In: Proceedings of the Royal Society of London B: Biological Sciences, 280(1771), 20131219.
  11. Ebrahimi, S. (2012). Study on genetic diversity of Paecilomyces variotii isolates in Kerman province. M.Sc. thesis. Departmant of Plant Protection, University of Zabol, Iran.
  12. Ershad, D. (2009). Fungi of Iran. (3rd ed.). Iranian Research Institute of Plant Protection, Tehran, Iran.
  13. Futuyma, D. J. (1998). Evolutionary biology. Sinauer Associates, Inc.
  14. Ghelichi, M., Mohammadi, A., Haghdel, M. & Eskandari, A. (2012). Distribution of pistachio die-back in Khorasan-Razavi province and application of some fungicides for the disease control. International Journal of Nuts and Related Sciences, 3(1), 23-28.
  15. Haghdel, M. (2008). Pistachio die back disease in Iran. 1st ed., Iranian Pistachio Research Institute.
  16. Halama, P. & Lacoste, L. (1992). Determination of sexual reproduction of Phaeosphaeria (Leptosphaeria) nodorum agent of septoriose of wheat. Action de la temperature et de la lumière. Canadian Journal of Botany, 70, 1563-1569.
  17. Heidarian, R., Fotouhifar, Kh.-B., Mohammadi, A. H., Javan-Nikkhah, M., Debets, A. J. M. & Aanen, D. K. (2015a). Host diversity and distribution of Paecilomyces formosus, the causal agent of pistachio die-back disease in Iran. In: Proceedings of 2nd Iranian Mycological Congress, 23-25 Aug., University of Tehran, Karaj, Iran, P. 210.
  18. Heidarian, R., Fotouhifar, Kh.-B., Mohammadi, A. H., Javan-Nikkhah, M., Debets, A. J. M. & Aanen, D. K. (2015b). Phylogeny of genus Paecilomyces sp., the causal agent of Pistachio die-back disease in Iran. In: Proceedings of 2nd Iranian Mycological Congress, 23-25 Aug., University of Tehran, Karaj, Iran, P. 30.
  19. Heitman, J., Sun, S. & James, T. Y. (2013). Evolution of fungal sexual reproduction. Mycologia, 105(1), 1-27.
  20. Houbraken, J., Samson, R. A. & Frisvad, J. C. (2006). Byssochlamys: significance of heat resistance and mycotoxin production. Advances in Experimental Medicine and Biology, 571, 211-224.
  21. Houbraken, J., Varga, J., Rico-Munoz, E., Johnson, S. & Samson, R. A. (2008). Sexual reproduction as the cause of heat resistance in the food spoilage fungus Byssochlamys spectabilis (anamorph Paeclomyces variotii). Applied and Environmental Microbiology, 74(5), 1613-1619.
  22. Houbraken, J., Verweij, P. E., Rijis, A. J. M. M., Borman, A. M. & Samson, R. A. (2010). Identification of Paecilomyces variotii in clinical samples and settings. Journal of Clinical Microbiology, 48(8), 2754-2761.
  23. Iranian Pistachio Society Journal. (2014). Pistachio. Retrieved March 18, 2015, from www.Iranpistachio.org.
  24. Jamali, S. & Banihashemi, Z. (2011). First report of Byssochlamys spectabilis as a teleomorph of Paecilomyces variotii from Iran. Rostaniha,12(2), 191-192.
  25. Luangsa-ard, J. J. & Hywel-Jones, N. L. (2004). The polyphasic nature of Paecilomyces sensu lato based on 18S-generated rDNA phylogeny. Mycologia, 96(4), 773-780.
  26. McCarthy, C. (1996). Chromas: version 1.3. Brisbane, Griffith University
  27. Mohammadi, A. & Haghdel, M. (2006). Diseases of dried fruit trees in temperate areas. (1st ed.). IPRI.
  28. Mozafari, V., Malakouti, M. J., Kholdebarin, B. & Bybordi, M. (2006). Investigation of some causes of die-back disorder of pistachio trees and its control through balanced fertilization in southern Iran. Iranian Journal of Soil and Waters Sciences, 19(2), 154-164. (in Farsi)
  29. Mostowfizadeh Galamfarsa, R. & Habibi, A. (2010). Essential fungal genetics. (1st ed.). Academic center for education, culture and research of Mashhad.
  30. Ni, M., Feretzaki, M., Sun, S., Wang, X. & Heitman, J. (2011). Sex in fungi. Annual Review of Genetics, 45, 405- 430.
  31. Oligo Analyzer 3.1 [online software]. (2014). Retrieved July 10, 2014, from eu.idtdna.com.
  32. Offerman, J. D. & Rychlik, W. (2003). Oligo Primer Analysis Software. In Krawetz, S. A. and Womble, D. D. (Ed), Introduction to bioinformatics: a theoretical and practical approach. (pp. 345-361) Humana Press Inc.
  33. Primer3web, version 4.0.0 [online software]. (2013). Retrieved July 10, 2014, from Primer3.ut.ee.
  34. Samson, R. A. (1974). Paecilomyces and some allied Hyphomycetes. Studies in Mycology, 6, 1-119.
  35. Samson, R. A., Houbraken, J., Varga, J. & Frisvad, J. C. (2009). Polyphasic taxonomy of the heat resistant ascomycete genus Byssochlamys and its Paecilomyces anamorphs. Persoonia, 22, 14-27.
  36. Solomon, P. S., Parker, K., Loughman, R. & Oliver, R. P. (2004). Both mating types of Phaeosphaeria (anamorph Stagonospora) nodorum are present in Western Australia. European Journal of Plant Pathology, 110, 763-766.
  37. Untergasser, A., Cutcutache, L., Koressaar, T., Ye, J., Faircloth, B. C., Remm, M. & Rozen, S. G. (2012). Primer3-new capabilities and interfaces. Nucleic Acids Research, 40(15), 115.
  38. Yun, S. H., Berbee, M. L., Yoder, O. C. & Turgeon, B. G. (1999). Evolution of the fungal self-fertile reproductive life style from self-sterile ancestors. In: Proceedings of the National Academy of Sciences, 96, 5592-5597.
Iranian Journal of Plant Protection Science
Volume 48, Issue 2 - Serial Number 2
February 2018
Pages 295-305

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History

  • Receive Date: 28 May 2016
  • Revise Date: 19 November 2016
  • Accept Date: 21 November 2016

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