Iranian Journal of Plant Protection Science

Iranian Journal of Plant Protection Science

Diversity between different Gibberella fujikuroi isolates on their gibberellin hormone production and disease severity in rice plant

Document Type : Research Paper

Authors
Sara Efati lakeh 1
Fereidoun Ppadasht-Dehkaei 2
Hossein Saremi 3
1 Former M.Sc. Student, Department of Plant Pathology, Faculty of Agriculture, University of Zanjan, Iran
2 Assistant Professor, Department of Plant Protection, Rice Research Institute, Rasht, Iran
3 Professor, Department of Plant Protection, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijpps.2018.203699.1006704
Abstract
Bakanae disease of rice is one of the most important rice diseases all over the worlds. Elongation is one of the conspicuous symptoms of Bakanae disease that is caused by gibberellins-producing isolates of the pathogen. The present investigation was done to reconsider the species of Bakanae, the ratio of pathogen’s species, virulence, gibberellin production and the relationship between virulence and the rate of gibberellin production in 23 isolates by gathering 50 samples of infected plants from rice fields. In order to identify the disease severity, inoculation of the plant stem was done by injection of spore suspension at the seedling stage in the Gohar variety. Results led us to identify two species of pathogen Fusarium fujikuroi and Fusarium verticillioides, and each one of them had a population of 88.4 and 11.6%, respectively. Results of the pathogenicity test showed that inoculation of the seedling causes infection in all the plant. Furthermore, the disease severity caused by the tested isolates were different in all the host plants. Results from tests of gibberellin determination using spectrophotometry and HPLC methods revealed that all the isolates were able to produce the hormone but the amount of it differed among isolates. No significant correlation was observed between the amount of gibberellin and percentage of stem elongation (disease severity). Perhaps, it may be due to early effects of virulence ability of the pathogen in comparison with late effects of gibberellin in the host plant. This leads to a lack of relationship between gibberellin levels and the disease severity of the pathogen.
Keywords
Fusarium fujikuroi
gibberellic acid
HPLC
spectrophotometry
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Iranian Journal of Plant Protection Science
Volume 49, Issue 1 - Serial Number 1
September 2018
Pages 81-90

  • Receive Date 03 April 2016
  • Revise Date 30 January 2018
  • Accept Date 23 April 2018