Assessments of formulated bacterial strains against crown and root rot of pistachio trees

Document Type : Research Paper


1 Former M. Sc. Student, Plant Protection Department, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran

2 Associate Professor, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran

3 Assistant Professor, Pistachio Research Center, Horticultural Science Research Instittute, Agriculture Research, Education and Extension Organization (AREEO), Rafsanjan, Iran


Gummosis (Crown and root rot) is the most important disease of pistachio trees caused by different species of Phytophthora which destroy significant numbers of fertile and non-fertile trees each year in Iran. Different approaches have been applied to reduce the severity and incidence of the disease. In recent years, biological control as an eco-friendly approach has been increased in the world. The effectiveness of Talk powder and Wheat bran formulations of five Pseudomonas strains (T17-4, VUPF760, VUPF5, VUPF506, and CHA0) was assessed on seedlings inoculated with Phytophthora drechsleri under greenhouse conditions. The highest and lowest reduction rates of disease severity belonged to VUPF760 and VUPF506 with 55 and 11%, respectively compared to the control and other treatments. Seedling inoculated with bacterial strains had higher heights, fresh and dry weights of shoot and roots. VUPF5 increased the ratios of height, fresh and dry weight of shoot and root by 1.4, 1.3, 1.1, 1.2, and 1.1 respectively, compared to non-inoculated control seedlings.


  1. Abbas-Zadeh, P., Saleh-Rastin, N., Asadi-Rahmani, H., Khavazi, K., Soltani, A., Shoary-Nejati, R. & Miransari, M. (2010). Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta Physiologiae Plantrum, 32, 281-288.
  2. Adesemoye, A. O. & Kloepper, J. W. (2009). Plant microbe’s interactions in enhanced fertilizer-use efficiency. Applied Microbiology and Biotechnology, 85, 1-12.
  3. Ahmadzadeh, M., Sharifi Tehrani, A. & Talebi Jahromi, K. (2004). Study on Production of Some Antimicrobial Metabolites by flourescent pseudomonads. Iranian Journal of Agriculture Science, 35(3), 731-45.
  4. Bora, T., Ozaktan, H., Gore, E. & Aslan, E. (2004). Biological control of Fusarium oxysporum f. sp. melonis by wettable powder formulations of the two strains of Pseudomonas putida. Journal of Phytopathology, 152, 471-475.
  5. Chiou, A. L. & Wu, W. S. (2003). Formulation of Bacillus amyloliquefaciens B190 for control of lily grey mould (Botrytis elliptica). Journal of Pathology, 151, 13-18.
  6. Fallahzadeh-Mamaghani, V., Ahmadzadeh, M. & Sharifi, R. (2009). Screening systemic resistance-inducing fluorescent pseudomonads for control of bacterial blight of cotton caused by Xanthomonas campestris pv. malvacearum. Journal of Plant Pathology, 91, 663-670.
  7. Glick, B. R. (2014). Bacteria with ACC deaminase can promote plant growth and help tofeed the world. Microbiological Research, 169, 30-39.
  8. Gupta, C. P., Dubeg, R. C., Kang, S. C. & Maheshwari, K. (2001). Antibiosis mediated necro trophic effect of Pseudomonas GRCz against two fungal plant pathogens. Current Science, 81(1), 91-94.
  9. Hokeberg, M., Gerhardson, B. & Johnsson, L. (1997). Biological control of cereal seed-borne diseases by seed bacterization with greenhouse-selected by bacteria. European Journal of Plant Pathology, 103, 25-33.
  10. Holmes, K. A. & Benson, D. M. (1994). Evaluation of Phytophthora parasitica var. nicotianae for biocontrol of Phytophthora parasitica on Catharanthus roseus. Plant Disease Journal, 78(2), 193-199.
  11. Kamilova, F., Validov, S., Azarova, T., Mulders, I. & Lugtenberg, B. (2005). Enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria. Environmental Microbiology, 7 , 1809-1817.
  12. Khatamidoost, Z., Jamali, S., Moradi, M. & Saberi Riseh, R. (2014). Effect of Iranian strains of Pseudomonas spp. on the control of root-knot nematodes on Pistachios. Biocontrol Science and Technology, 25, 291-301.
  13. Kloepper, J.W. (1993). Plant Growth-promoting Rhizobacteria as biological control agents. In: F. Blaine Metling, Jr. (Ed.), Soil Microbial Ecology, Application in Agricultural and Environmental Management. New York: Marcel Dekker Inc., pp. 255-274.
  14. Lagzian, A., SaberiRiseh, R., Khodaygan, P., Sedaghati, E. & Dashti, H. (2013). Introduced Pseudomonas fluorescens VUPf5 as an important biocontrol agent for controlling Gaeumannomyces graminis var. tritici the causal agent of take-all disease in wheat.Archives of Phytopathology and Plant Protection, 1, 1-5.
  15. Liu, H., He, Y., Jiang, H., Peng, H., Huang, X., Zhang, X., Thomashow, L. S. & Xu, Y. (2007). Characterization of a phenazine- producing strain Pseudomonas chlororaphis GP72 with broad-spectrum antifungal activity from green pepper rhizosphere. Current Microbiology, 54, 302-306.
  16. Ma, Y., Prasad, M. N. V. Rajkumar, M. & Freitas, H. (2011). Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnology Advances, 29, 248-258.
  17. Mahmudi mimand, B., Saberi Ryseh, R., Moradi, M., Alaei, H. & Mohammadi, A. H. (2016). Induction of plant defense response against Phytophthora crown and root rot in pistachio by Pseudomonas fluorescens strains. Iranian Journal of Plant Protection Science, 47(1), 103-105. (in Farsi)
  18. Melin, P., Hakansson, S. & Schnurer, J. (2007). Optimisation and comparison of liquid and dry formulations of the biocontrol yeast Pichia anomala J121. Applied Microbiology and Biotechnology, 73, 1008-1016.
  19. Mirabolfathy, M., Cooke, D. E. L., Duncan, J. M., Williams, N. A., Ershad, J. & Alizadeh, A. (2001). Phytophthora pistaciae sp. nov. and P. melonis the principal causes of pistachio gummosis in Iran. Mycological Research, 105, 1166-1175.
  20. Moradi, M. (1998). Isolation and identification of Phytophthora species from root and crown of pistachio in Kerman and Fars provinces and resistance determination of root and crown among current pistachio cultivars. M.Sc. dissertation, Faculty of Agriculture, Shiraz University, Iran. (in Farsi)
  21. Nakkeeran, S., Renukadev, P. & Marimuthu, T. (2005). Antagonistic potentiality of Trichoderma viride and assessment of its efficacy for the management of cotton root rot. Archives of Phytopathology and Plant Protection, 38(3), 209-225.
  22. Roberts, M. A. (2002). Actinomycetes, biocontrol, questions and answers. Available on internet at:
  23. Sabaratnam, S. & Traquair, J. A. (2002). Formulation of a streptomyces biocontrol agent for the suppression of rhizoctonia damping-off in tomato transplants. Biological Control, 23, 245-253.
  24. Saberi-Riseh, R., Sharifi-Tehrani, A., Khezri, M., Ahmadzadeh, M. & Nikkhah, M. J. (2006). Study on biocontrol of Phytophthora citrophthora, the causal agent of pistachio gummosis. Acta Horticulture (ISHS), 726, 627-630.
  25. Shahidi Bonjar, G. H., Barkhordar, B., Pakgohar, N., Aghighi, S., Biglary, S., Rasid Farrokhi, P., Aminaii, M., Mahdavi, M. J. & Aghelizadeh, A. (2006). Biological control of Phytophthora drechsleri Tucker, The causal agent of pistachio gummosis, under greenhouse conditions by use of Actinomycetes. Journal of Plant Pathology, 5(1), 20-23.
  26. Shahidi Bonjar, G. H., Fooladi, M. H., Mahdavi, M. J. & Shahghasi, A. (2004). Broadspectrim, a novel antibacterial from Streptomyces sp. Biotechnology, 3, 126-130.
  27. Shirzad, A., Fallahzadeh-Mamaghani, V. & Pazhouhandeh, M. (2012). Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri. Plant Pathology Journal, 28(1), 1-9.
  28. Tahmasbi, F., Lakzian, A., Khavazi, K. & Pakdin Parizi, A. (2014). Isolation, identification and evaluation of siderophore production in Pseudomonas bacteria and its effect on hydroponically grown corn. Journal of Cell and Molecular Research, 87-75, 1, 27.