Iranian Journal of Plant Protection Science

Iranian Journal of Plant Protection Science

Optimizing suspensibility, stability and virulence of commercial products of Bacillus thuringiensis

Document Type : Research Paper

Authors
Zahra Ashjaei
Reza Talaei-Hassanloui
Ayda Khorramnejad
Khalil Talebi Jahromi
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijpps.2020.301765.1006942
Abstract
The purpose of this study was to determine the effect of some additives on stability and suspensibility of wettable powder (WP) formulation of Bacillus thuringiensis (Bt) in laboratory products (optimized formulation) and commercial formulations. For preparation of optimized formulation; sucrose, lactose, sodium alginate, coconut fiber and aluminum silicate were added to the dried powder of bacterial product in two different treatments; which were different in aluminum silicate (optimized formulation 1) and coconut fiber (optimized formulation 2). The efficacy and toxicity of wettable powders were tested on diamondback moth, Plutella xylostella (L.) (Lep.: Plutellidae) larvae. The results of various tests for stability showed that the additives had positive effects on the storage life of the optimized formulation. The suspensibility of the optimized formulations (70%) was significantly higher than those of the commercial formulations. Our results showed that adding sucrose in both commercial products caused an increase in toxicity (lower LC50s), whereas, the suspensibility parameter varied depending on the type and feature of the products. In conclusion, selecting low-cost and available additives would improve the physical and biological characteristics of Bt formulations, which has a significant effect on the development and application of biopesticides.
Keywords
wettable powder
optimization
sucrose
formulation
Plutella xylostella
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Iranian Journal of Plant Protection Science
Volume 52, Issue 1
September 2021
Pages 81-89

  • Receive Date 31 May 2020
  • Revise Date 31 July 2020
  • Accept Date 04 August 2020