The purpose of this study was to determine the effect of some additives on stability and suspensibility of wettable powder (WP) formulation of Bacillus thuringiensis (Bt) in laboratory products (optimized formulation) and commercial formulations. For preparation of optimized formulation; sucrose, lactose, sodium alginate, coconut fiber and aluminum silicate were added to the dried powder of bacterial product in two different treatments; which were different in aluminum silicate (optimized formulation 1) and coconut fiber (optimized formulation 2). The efficacy and toxicity of wettable powders were tested on diamondback moth, Plutella xylostella (L.) (Lep.: Plutellidae) larvae. The results of various tests for stability showed that the additives had positive effects on the storage life of the optimized formulation. The suspensibility of the optimized formulations (70%) was significantly higher than those of the commercial formulations. Our results showed that adding sucrose in both commercial products caused an increase in toxicity (lower LC50s), whereas, the suspensibility parameter varied depending on the type and feature of the products. In conclusion, selecting low-cost and available additives would improve the physical and biological characteristics of Bt formulations, which has a significant effect on the development and application of biopesticides.
Bradford, M.M. (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry, 72 (1–2): 248–254, doi:1016/0003-2697(76)90527-3.
Bryant, J .(1994). Commercial production and formulation of Bacillus thuringiensis. Agriculture, Ecosystems & Environment ,49(1): 31-35.
Brar, SK., Verma, M., Tyagi, R., Valéro, J. (2006 a). Recent advances in downstream processing and formulations of Bacillus thuringiensis based biopesticides. Process Biochemistry ,41(2): 323-342.
Brar, SK., Verma, M., Tyagi, R., Valéro J., Surampalli, RY .(2005). Starch Industry Wastewater-Based Stable Bacillusthuringiensis Liquid Formulation. Journal of Economic Entomology ,98(6):1890-1898.
Brar, SK., Verma, M., Tyagi, R., Valéro, J., Surampalli, RY. (2006 b). Screening of Different Adjuvants for Wastewater/Wastewater Sludge-Based BacillusthuringiensisJournal of Economic Entomology ,99(4):1065-1079.
Burges, HD., Jones, KA. (1998) Formulation of bacteria, viruses and protozoa to control insects, In Burges HD (Ed.), Formulation of microbial biopesticides: beneficial organisms, nematodes & seed treatments.(pp.34-109). Kluwer Academic Publishers, Netherlands.
Corrêa, EB., Sutton, JC., Bettiol, W. (2015). Formulation of Pseudomonaschlororaphis strains for improved shelf life. Biological Control,80 :50–55.
Couch, TL., Jurat-Fuentes, JL. (2013). Commercial Production of Entomopathogenic Bacteria,In: Morales-Ramos, JA,. Rojas, MG.and Shapiro-Ilan, DI (Ed.), Massproductionofbeneficialorganisms: invertebrates and entomopathogens. 1st Edition.(pp.415-436). Academic Press, United States of America.
El‐Hassan, S., Gowen, S. (2006). Formulation and delivery of the bacterial antagonist Bacillussubtilis for management of lentil vascular wilt caused by Fusariumoxysporum sp. lentis. Journal of Phytopathology, 154(3): 148-155.
Glare, TR., Ocallaghan, M. (2000) Bacillus thuringiensis : biology, ecology and safety. New York: Wiley.
Goerge, Z., Crickmore, N. (2012). Bacillus thuringiensis Application in Agriculture, In: Sansinenea (Ed.), Bacillus thuringiensis(pp.19-39). Springer, Netherlands.
Jallouli, W., Sellami, S., Sellami, M., Tounsi, S. (2014). Efficacy of olive mill wastewater for protecting Bacillusthuringiensis formulation from UV radiations. Acta Tropica, 140: 19–25.
Jayaraj, J., Kannan,R., Sakthivel, K., Suganya, D., Venkatesan, S., Velazhahan, R. (2005). Development of new formulations of Bacillussubtilis for management of tomato damping-off caused by Pythiumaphanidermatum. Biocontrol Science and Technology, 15(1): 55-65.
Khaliq, MSAA., Haque, M .(2007). Scope of commercial formulations of Bacillusthuringiensis Berliner as an alternative to methyl bromide against Trogodermagranarium Everts larvae. Pakistan Journal of Botany, 39(3): 871-880.
Khoramnezhad, A., Talaei-Hassanloui, R., Ghassemi-Kahrizeh, A. (2015). Evaluating the virulence of Bacillus thuringiensis strains isolated from host and different habitats on diamondback moth, Plutella xylostella (Lep.: Plutellidae). Biological Control of Pests and Plant Diseases, 4(2): 168-172. (In Farsi)
Khorramvatan, S., Marzban, R., Ardjmand, M., Seifkordi, A., Askary, H. (2014) .Preparation of concentrated suspension of microencapsulated formulation of Bacillus thuringiensis. Biocontrol in Plant Protection ,2 (1):81-89. In Farsi.
Koul, O. (2011). Microbial biopesticides: opportunities and challenges. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 6(056): 1-26.
Lacey, LA., Frutos, R., Kaya, HK., Vail, P. (2001). Insect pathogens as biological control agents: do they have a future?. Biological Control, 21(3): 230-248.
Morris, ON., Convers, V., Kanagratnam, P. (1995). Chemical additive effects on the efficacy of B.t. var. kurstaki against Mamestriaconfigurata. Journal of Economic Entomology, 88(4): 815-824.
Palma, L .(2017). Bacillusthuringiensis-based biopesticides, are they as effective as they should be?. Revista Argentina de microbiologia, 49(1): 119.
Rosas-García, NM., Villegas-Mendoza, JM.,Torres-Ortega JA. (2009). Design of a Bacillusthuringiensis-based formulation that increases feeding preference on Spodopteraexigua (Lepidoptera: Noctuidae) larvae. Journal of Economic Entomology, 102(1): 58-63.
Salehi Jouzani, GH., Moaven, E., Morsali, H. (2014). Optimization of a wettable powder formulation for two native BacillusthuringiensisBiological Control of Pests and Plant Diseases, 3(1): 7-15. (In Farsi)
Singh, A., Boora, SK., Chaudhary, K .(2007). Effect of different additives on the persistence and insecticidal activity of native strains of BacillusIndian Journal of Microbiology, 47(1): 42–45.
Sneh, B., Schuster, S., Gross, S. (1983). Improvement of the insecticidal activity of Bacillusthuringiensisentomocidus on larvae of Spodoptera littoralis (Lepidoptera, Noctuidae) by addition of chitinolytic bacteria, a phagostimulant and a UV‐protectant. Journal of Applied Entomology, 96(1-5): 77-83.
Sun, S., Cheng, Z., Fan, J., Cheng, X., Pang, Y. (2012). The utility of camptothecin as a synergist of Bacillus thuringiensiskurstaki and nucleopolyhedroviruses against Trichoplusia ni and Spodoptera exigua. Journal of Economic Entomology, 05(4): 1164-1170.
Talekar, N., Shelton, A. (1993). Biology, ecology, and management of the diamondback moth. Annual Review of Entomology, 38: 275-301.
Teera-Arunsiri, A., Suphantharika, M., Ketunuti, U. (2003). Preparation of spray-dried wettable powder formulations of Bacillus thuringiensis-based biopesticides. Journal of Economic Entomology, 96(2): 292-299.
Zouari, N., Achour O., Jaoua S. (2002). Production of delta‐endotoxin by Bacillusthuringiensis subsp kurstaki and overcoming of catabolite repression by using highly concentrated gruel and fish meal media in 2‐ and 20‐dm3 fermenters. Journal of Chemical Technology and Biotechnology, 77(8): 877-882.
Zhang, L., Zhang, X., Zhang, Y., Wu, S., Gelbic, I., Xu, L., Guan, X. (2016). new formulation of Bacillusthuringiensis: UV protection and sustained release mosquito larvae studies. Scientific Reports, 6: 39425.
Ashjaei, Z., Talaei-Hassanloui, R., Khorramnejad, A., & Talebi Jahromi, K. (2021). Optimizing suspensibility, stability and virulence of commercial products of Bacillus thuringiensis. Iranian Journal of Plant Protection Science, 52(1), 81-89. doi: 10.22059/ijpps.2020.301765.1006942
MLA
Zahra Ashjaei; Reza Talaei-Hassanloui; Ayda Khorramnejad; Khalil Talebi Jahromi. "Optimizing suspensibility, stability and virulence of commercial products of Bacillus thuringiensis", Iranian Journal of Plant Protection Science, 52, 1, 2021, 81-89. doi: 10.22059/ijpps.2020.301765.1006942
HARVARD
Ashjaei, Z., Talaei-Hassanloui, R., Khorramnejad, A., Talebi Jahromi, K. (2021). 'Optimizing suspensibility, stability and virulence of commercial products of Bacillus thuringiensis', Iranian Journal of Plant Protection Science, 52(1), pp. 81-89. doi: 10.22059/ijpps.2020.301765.1006942
VANCOUVER
Ashjaei, Z., Talaei-Hassanloui, R., Khorramnejad, A., Talebi Jahromi, K. Optimizing suspensibility, stability and virulence of commercial products of Bacillus thuringiensis. Iranian Journal of Plant Protection Science, 2021; 52(1): 81-89. doi: 10.22059/ijpps.2020.301765.1006942