Isolation, identification and dissemination of Fusarium species from soil of two different climates in Afghanistan

Document Type : Research Paper

Authors

1 department of plant protection, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

2 department of plant protection, College of Agriculture & Natural Resources, University of Tehran,Karaj-Iran

3 Professor/Department of Plant Protection, College of Agriculture and Natural Science,, University of Tehran, Karaj, Iran

4 Department of Plant Protection, College of Agriculture and Natural Science,, University of Tehran, Karaj, Iran

5 Associate Professor, College of Agriculture, University of Bamyan, Afghanistan

Abstract

The members of Fusarium is one of the important causal egent of soil borne diseases which cause serious damage in the agricultural and horticultural crops in the world. In order to investigate Fusarium destributions in agricultural soils in the Bamyan and Daikundi with semi desert climates, Kandahar and Helmand with desert climates, sampling was performed in Afghanistan in 2018-2019. The PPA medium (Pepton PCNB Agar) was used for Fusarium isolations. For morphological characterisation, pure cultures were transferred to CLA (Carnation Leaf-piece Agar), SNA (Synthetic Nutrient Agar ) and PDA (Potato Dextrose Agar) mediums and gene sequencing of tef-lα regions were performed for molecular identification. Totally 134 Fusarium isolates were obtained and grouped in 11 different species. The presence of F. incarnatum-equiseti species complex (FIESC) (28%), F. solani (16%), F. acuminatum (6%), F.verticillioides (5%), F. oxysporum (3%), F. coeruleum (1%) and F. compactum (1%) with different frequency were detected in both regions of the sampling areas. F. sambucinum (1%) was detected in semi-desert climates (Bamyan and Daikundi provinces), While F. proliferatum (18%), F. culmorum (13%) and F. Pseudograminearum (1%) were isolated and identified in areas with desert climates (Helmand and Kandahar provinces). The results this study, geographical distribution of various species of Fusarium in Afghanistan, provide useful information about management of soil borne Fusarium pathogens.

Keywords

References

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Iranian Journal of Plant Protection Science
Volume 52, Issue 1
September 2021
Pages 149-159

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History

  • Receive Date: 18 October 2020
  • Revise Date: 05 January 2021
  • Accept Date: 07 March 2021

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