Iranian Journal of Plant Protection Science

Iranian Journal of Plant Protection Science

Identification of Didymellaceae fungi associated with different disease symptoms in some medicinal plants of Khuzestan Province, Iran

Document Type : Research Paper

Authors
Fatemeh Keshavarzi
Reza Farrokhinejad
Mehdi Mehrabi-Koushki
Department of Plant protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz
10.22059/ijpps.2022.341338.1007005
Abstract
Bitter apple (Citrullus colocynthis), camelthorn (Alhagi sp.), cordia (Cordia myxa), dill (Anethum graveolens), eucalyptus (Eucalyptus sp.), licorice (Glycyrrhiza glabra), plantain (Plantago sp.), and rosemary (Rosmarinus officinalis) are medicinal plants distributed in different parts of Khuzestan province. These plants could be host to many pathogenic and non-pathogenic fungi. In this study, 30 samples from medicinal plants showing leaf spot, stem canker and stem necrosis were collected during 2019-2020, and in which 23 isolates of six species in the family Didymellaceae were identified. Molphological characteristics were studied on potato – dextrose - agar (PDA). For molecular study, partial regions of tub2 gene were amplified using appropriate primers and sequenced. Based on molecular phylogeny in combination with morphology, the isolates were identified as follow: Allophoma labilis, Didymella glomerata, Epicoccum italicum, Neodidymelliopsis farokhinejad, Nothophoma raii and Xenodidymella glycyrrhizicola. In our knowledge, this is the first report of above species on surveyed hosts and first record of both E. italicum and N. raii for mycobiota of Iran.
Keywords
Molecular phylogeny
morphology
pycnidium
conidium
plant host
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Iranian Journal of Plant Protection Science
Volume 53, Issue 2
January 2023
Pages 163-176

  • Receive Date 18 April 2022
  • Revise Date 23 July 2022
  • Accept Date 13 September 2022