Detection and distribution of Barley yellow dwarf virus (BYDV) in some cereal fields of Hamedan province

Document Type : Research Paper


Agriculture faculty of Bu-ali Sina university- Hamadan- Iran


Barley yellow dwarf disease is one of the most prevalent and economically important virus disease of cereals. Barley yellow dwarf virus, BYDV belongs to the family Luteoviridae is causal agent of barely yellow dwarf disease which can result up to 80% yield reduction in wheat. BYDV is present in most regions of Iran. For detection and study on distribution of BYDV, wheat and barley plants showing yellows and dwarfing symptoms were collected from fields of Malayer, Nahavand and Tuyserkan during growing season of 2020-2021. The samples were checked for BYDV infection using indirect-ELISA. The results showed presence of BYDV in most samples. Positive reactions to BYDV antibody in 80% of the Malayer samples, 90% of the Nahavand samples and 80% of the Tuyserkan samples were recorded. Also, total RNA extraction and RT-PCR were performed using specific BYDV-PAV primers; the most prevalent strain of BYDV in most regions of Iran. To verify the results, a sample with symptoms from Tuyserkan selected for sequencing. The BYDV isolate of Tuyserkan had 97% of homology with BYDV isolates were reported from Kahnouj, Karaj, Yazd and Arak, while it showed the least similarity with other Iranian isolates (about 85%).


  1. Adhikari, A., Lockhart, B. E., Ganiger, M., Byamukama, E., Tande, C., Smith, M. J. & Dill-Macky, R. (2020). Barley yellow dwarf virus-PAV is the dominant species causing Barley yellow dwarf disease in South Dakota and Minnesota. Crop Protection, 134, 105171.
  2. Adhikari, A., Steffenson, B. J., Smith, M. J. & Dill‐Macky, R. (2019). Genome‐wide association mapping of seedling net form net blotch resistance in an Ethiopian and Eritrean barley collection. Crop Science, 59(4), 1625-1638.
  3. Aradottir, G. I. & Crespo-Herrera, L. (2021). Host plant resistance in wheat to barley yellow dwarf viruses and their aphid vectors: a review. Current Opinion in Insect Science, 45, 59-68.
  4. Bisnieks, M., Kvarnheden, A., Sigvald, R. & Valkonen, J. (2004). Molecular diversity of the coat protein-encoding region of Barley yellow dwarf virus-PAV and Barley yellow dwarf virus-MAV from Latvia and Sweden. Archives of virology, 149(4), 843-853.
  5. Choudhury, S., Hu, H., Meinke, H., Shabala, S., Westmore, G., Larkin, P. & Zhou, M. (2017). Barley yellow dwarf viruses: infection mechanisms and breeding strategies. Euphytica, 213(8), 1-22.
  6. Choudhury, S., Larkin, P., Meinke, H., Hasanuzzaman, M., Johnson, P. & Zhou, M. (2019). Barley yellow dwarf virus infection affects physiology, morphology, grain yield and flour pasting properties of wheat. Crop and Pasture Science, 70(1), 16-25.
  7. Converse, R. & Martin, R. (1990). ELISA methods for plant viruses. Serological methods for detection and identification of viral and bacterial plant pathogens, a Laboratory Manual, 179-196.
  8. Domier, L. & D'Arcy, C. (2008). Luteoviruses. In Encyclopedia of virology (pp. 231-238). Elsevier Ltd.
  9. Domier, L. L. (2008). Barley yellow dwarf viruses. In Encyclopedia of virology (pp. 279-286). Elsevier Ltd.
  10. Domier, L. L. (2021). Barley Yellow Dwarf Viruses (Luteoviridae). In Encyclopedia of Virology (4th ed.) (pp. 81-97). Elsevier Ltd.
  11. Izadpanah, K. & Lister, R. (1991). Identification of barley yellow dwarf virus serotypes in Iran. Proceedings of the 10th Plant Protection Congress of Iran 1-5 Sept. 1991, Kerman (Iran Islamic Republic).(In Farsi)
  12. Jarošová, J., Singh, K., Chrpová, J. & Kundu, J. K. (2020). Analysis of small RNAs of barley genotypes associated with resistance to Barley Yellow Dwarf Virus. Plants, 9(1), 60.
  13. Kaddachi, I., Souiden, Y., Achouri, D. & Chéour, F. (2014). Barley yellow dwarf virus (BYDV): characteristics, hosts, vectors, disease symptoms and diagnosis. International Journal of Phytopathology, 3(3), 155-160.
  14. Lotfipour, M., Behjatnia, A., Afsharifar, A. & Izadpanah, k. (2012). Distribution and partial biological characterization of wheat and barley strains of wheat dwarf virus in Iran. Iranian Journal of Plant Pathology, 49(1), 17-31.(In farsi)
  15. Oswald, J. W. & Houston, B. (1951). A new virus disease of cereals, transmissible by aphids. Plant Disease Reporter, 11, 471-475.
  16. Pakdel, A., Afsharifar, A., & Niazi, A. (2015). Molecular characterization of the complete genome of a barley yellow dwarf virus-PAV isolate from Iran. Iranian Journal of Plant Pathology, 51(2), 163-176.(In farsi)
  17. Pakdel, A., Afsharifar, A., Niazi, A., Almasi, R. & Izadpanah, K. (2010). Distribution of cereal Luteoviruses and molecular diversity of BYDV‐PAV Isolates in central and southern Iran: Proposal of a new species in the genus Luteovirus. Journal of phytopathology, 158(5), 357-364.
  18. Patil, B. L. (2020). Plant viral diseases: Economic implications. In Encyclopedia of virology (4th ed.). (pp. 81-97). Elsevier Ltd.
  19. Plan and budget organization,‌ statistical center of Iran, statistical yearbook 2018-2019, from
  20. Rastgou, M., Khatabi, B., Kvarnheden, A., & Izadpanah, K. (2005). Relationships of Barley yellow dwarf virus-PAV and Cereal yellow dwarf virus-RPV from Iran with viruses of the family Luteoviridae. European Journal of Plant Pathology, 113(3), 321-326.
  21. Riyaz, S. M., Jesse, D. M. I. & Kathiravan, K. (2021). Plant virus: Diversity and ecology. In Plant Virus-Host Interaction (pp. 319-328). Elsevier.
  22. Shen, C., Wei, C., Li, J., Zhang, X., Zhong, Q., Li, Y., Bai, B. & Wu, Y. (2020).Barley yellow dwarf virus-GAV-derived vsiRNAs are involved in the production of wheat leaf yellowing symptoms by targeting chlorophyll synthase. Virology Journal, 17(1), 1-14.
  23. Van den Eynde, R., Van Leeuwen, T. & Haesaert, G. (2020). Identifying drivers of spatio‐temporal dynamics in barley yellow dwarf virus epidemiology as a critical factor in disease control. Pest management science, 76(8), 2548-2556.
  24. Walls, J., Rajotte, E. & Rosa, C. (2019). The past, present, and future of barley yellow dwarf management. Agriculture, 9(1), 23.
  25. Wang, X., Rong, W., Liu, Y., Wang, X. & Zhang, Z. (2018). Investigation of the mechanism of adult-stage resistance to barley yellow dwarf virus associated with a wheat–Thinopyrum intermedium translocation. The Crop Journal, 6(4), 394-405.