Investigation on the effect of orange and tangerine nanoemulsions' controlled release on their fumigant toxicity against Tribolium confusum (Col.: Tenebrinoidea) and Sitophilus oryzae (Col: Curculionidae)

Articles in Press

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agricultural Science, University of Jiroft, Jiroft, Iran

2 General directorate inso kerman office,Kerman,Iran

3 Syngenta Company Ahvaz, Iran

4 Department of Chemistry, University of Jiroft, Jiroft, Iran

Abstract

Nanoemulsion formulation has been one of the most effective types of formulation to achieve controlled release of essential oils. In this study, in order to show the effect of controlled release, fumigant toxicity of essential oils and nanoemulsions from orange and tangerine peels were investigated on two storage pests, T. confusum and S. oryzae, in 24 and 48 hours. The essential oils were extracted from dried orange and tangerine peels using a hydrodistillation method and their content were analyzed by gas chromatography mass spectrometry (GC-MS). Ultrasonic probe sonicator (UPS) was utilized to convert the essential oil particles into the nanometer scale. Particle sizes were determined using a dynamic light scattering (DLS) instrument and was 65 nanometer. Based on preliminary experiments, eight concentrations (5, 10, 15, 20, 25, 50, 75, and 95 microliters) of the essential oils and nanoformulations were prepared for fumigant toxicity evaluation. The bioassays were conducted under controlled conditions at a temperature of 27±1 C◦, relative humidity of 65±5%, and with three independent replicates at 24 and 48 hours. Fumigant toxicity of the essential oils was higher at 24 hours, while the nanoemulsions exhibited greater efficacy at 48 hours. The highest observed mortality percentage was recorded for orange nanoemulsion at 48 hours and a concentration of 95 µl. Results showed higher efficiency of nanoemulsions in 48 h while they were less efficient than non-formulated essential oils in 24 h that is related to their better release function.

Keywords

Iranian Journal of Plant Protection Science

Articles in Press, Accepted Manuscript
Available Online from 18 September 2024

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History

  • Receive Date: 08 June 2024
  • Revise Date: 17 September 2024
  • Accept Date: 18 September 2024

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