Role of β-amino butyric acid (BABA) in the induction of resistance against cucumber downy mildew and activities of defense-related enzymes

Document Type : Research Paper

Authors

1 Former M. Sc. Student, Faculty of Agriculture, University of Jiroft, Iran

2 Assistant Professor, Faculty of Agriculture, University of Jiroft, Iran

3 Instructor, Faculty of Agriculture, University of Jiroft, Iran

Abstract

Cucumber downy mildew caused by Pseudoperonospora cubensis is one of the most important diseases of cucumberin Iran. In this investigation the role of β-Aminobutyric acid (BABA) in reducing this disease was studied via spraying of leaves and soil drench. The plants were treated with BABA and after 24 hours leaves were detached and inoculated with the pathogen and kept in a plastic box with favorable conditions for the disease agent. The disease was evaluated six days after inoculation with pathogen by scoring the leaves between 0-9 and the disease index calculated. Result showed that spraying the leaves by BABA had no effect on the disease reduction but soil application of this compound statistically reduced the disease (p≤0.05). The second phase of the experiment was done for evaluating the effect of BABA on the activity of enzymes such as β-1,4-Glucanase, phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase. Enzymes activity was assayed at 0, 24, 48, 72 and 96 hours after inoculation with the pathogen. Results showed an increas jn enzyme activity at 48-hours post-inoculation with the pathogen and then β-1,4-Glucanase, PAL, and POX enzymes activities decreased slowly, while PPO enzymes activity decreased rapidly.

Keywords

References

  1. Ahamad, S., Narain, U., Prajapati, R. & LAL, C. (2000). Management of downy mildew of Cucumber. Annals of Plant Protection Sciences, 8(2), 254-255.
  2. Alizadeh, H. & Salari, Kh. (2015). Induced resistance by β-amino butyric acid (BABA) against fusarium stem and root rot of cucumber. Iranian Journal of Plant Protection Science, 45(2), 299-307
  3. Altamiranda, E., Andrendreu, A., Daleoo, G. & Olivieri, F. (2008). Effect of β-aminobutyric acid (BABA) on protection against Phytophthora infestans throughout the potato crop cycle. Australasian Plant Pathology, 37(4), 421-427.
  4. Bagi, F. F., Budakov, D. B., Bursic, V. P., Stojsin, V. B., Lazic, S. D. & Vukovic, S. M. (2014). Efficacy of azoxystrobin for the control of cucumber downy mildew (Pseudoperonospora cubensis) and fungicide residue analysis. Crop Protection, 61(0), 74-78.
  5. Barilli, E., Rubiales, D. & Castillego, M. Á. (2012). Comparative proteomic analysis of BTH and BABA-induced resistance in pea (Pisum sativum) toward infection with Pea rust (Uromyces pisi). Journal of Proteomics, 75(17), 5189-5205.
  6. Bengtsson, T., Weighill, D., Proux-Wéra, E., Levander, F., Resjö, S., Burra, D. D., Moushib, L. I., Hedley, P. E., Liljeroth, E., Jacobson, D., Alexandersson, E. & Andreasson, E. (2014). Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach. BMC Genomics, 15(1), 315.
  7. Beudoin-Eagan, L. D. & Thorpe, T. A. (1985). Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiology, 78(3), 438-441.
  8. Benhamou, N. & Theriaul, G. (1992). Treatment with chitosan enhances resistance of Tomato plants to the crown and root rot pathogen Fusarium oxysporum f. sp. radicis-lycopersici. Physiological and Molecular Plant Pathology, 41(1), 33-52.
  9. Bhiyan, N. H., Selvarag, G., Wei, Y. & King J. (2009). Gene expression profiling and silencing reveal that monolignol biosynthesis plays a critical role in penetration defence in wheat against powdery mildew invasion. Journal of Experimental Botany, 60(2), 509-521.
  10. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry,72(1-2), 248-254.
  11. Chahance, B. & Maehly, A. (1955). Assay of catalases and peroxidases. Methods in Enzymology, 2, 764-775.
  12. Chen, C., Belanger, R. R., Benhamou, N. & Paulitz, T. C. (2000). Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiological and Molecular Plant Pathology, 56(1), 13-23.
  13. Chen, J. Y., Wen, P. F., Kong, W. F., Pan, Q. H., Zhan, J. C., Li, J. M., Wan, S. B. & Huang, W. D. (2006). Effect of salicylic acid on phenylpropanoids and phenylalanine ammonia-lyase in harvested grape berries. Postharvest Biology and Technology, 40(1), 64-72.
  14. Cohen, Y. (1994). 3-Aminobutyric acid induces systemic resistance against Peronospore tabacina. Physiological and Molecular Plant Pathology, 44(4), 273-288.
  15. Cohen, Y. (2001). The BABA story of induced resistance. Phytoparasitica, 29(5), 375-378.
  16. Cohen, Y., Niderman T., Mosinger, E. & Fluhr, R. (1994). beta-Aminobutyric acid induces the accumulation of pathogenesis-related proteins in Tomato (Lycopersicon esculentum L.) plants and resistance to late blight infection caused by Phytophthora infestans. Plant Physiology, 104(1), 59-66.
  17. Cohen, Y. Reuveni, M. & Baider, A. (1999). Local and systemic activity of BABA (DL-3-aminobutyric Acid) against Plasmopara viticola in Grapevines. European Journal of Plant Pathology, 105(4), 351-361.
  18. Cohen, Y. Rubin, A. & Vaknin, M. (2011). Post infection application of DL-3-amino-butyric acid (BABA) induces multiple forms of resistance against Bremia lactucae in lettuce. European Journal of Plant Pathology, 130(1), 13-27.
  19. Cohen, Y., Rubin, A. E. & Kilfin, G. (2009). Mechanisms of induced resistance in lettuce against Bremia lactucae by DL-β-amino-butyric acid (BABA). European Journal of Plant Pathology, 126(4), 553-573.
  20. Conrath, U., Pieterse, C. M. J. & Mauch-Mani, B. (2002). Priming in plant-pathogen interactions. Trends in Plant Science, 7(5), 210-216
  21. Godard, J. F., Ziadi, S. L., Monot, C., Corre, D. L. & Silué D. (1999). Benzothiadiazole (BTH) induces resistance in cauliflower (Brassica oleracea var botrytis) to downy mildew of crucifers caused by Peronospora parasitica. Crop Protection, 18(6), 397-405.
  22. Gozzo, F. (2003). Systemic acquired resistance in crop protection: from nature to a chemical approach. Journal of Agricultural and Food Chemistry, 51(16), 4487-4503.
  23. Hamiduzzaman, M. M., Jakab, G., Barnavon, L., Neuhaus, J. M. & Mauch-Mani, B. (2005). β-Aminobutyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling. Molecular Plant-Microbe Interactions, 18(8), 819-829.
  24. Hu, G. & Rijkenberg, F. H. (1998). Subcellular localization of β-1, 3-glucanase in Puccinia recondita f. sp. tritici-infected wheat leaves. Planta, 204(3), 324-334.
  25. Hwang, B.,  Ksunwoo, J. Y., Kim, Y. J. & Kim, B. S. (1997). Accumulation of β-1, 3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici. Physiological and MolecularPlant Pathology, 51(5), 305-322.
  26. Jakab, G., Cottier, V., Toquin, V., Rigoli, G., Zimmerli, L., Metraux, J. P. & Mauch-Mani, B. (2001). β-Aminobutyric Acid-induced Resistance in Plants. European Journal of Plant Pathology, 107(1), 29-37.
  27. Jeun, Y. C., Lee, Y. J. & Bae, Y. S. (2004). Rhizobacteria-mediated induced systemic resistance in cucumber plants against anthracnose disease caused by Colletotrichum orbiculare. Journal of Plant Pathology 20, 172-176.
  28. Justyna, P. G. & Ewa, K. (2013). Induction of resistance against pathogens by β-aminobutyric acid. Acta Physiologiae Plantarum, 35 (6), 1735-48.
  29. Kim Khiook, I. L., Schneider, C., Heloir, M. C., Bis, B., Daire X., Adrian, M. & Trovelot, S. (2013). Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: Application to the evaluation of resistance to downy mildew. Journal of Microbiological Methods, 95(2), 235-244.
  30. Koen, E., Trapet, P., Brule, D., Kulik, A., Klinguer, A., Atauri-Miranda, L., Meunier-Prest, R., Boni, G., Glauser, G., Mauch-Mani, B., Wendehenne, D. & Besson-Bard, A. (2014). beta-Aminobutyric acid (BABA)-induced resistance in Arabidopsis thaliana: link with iron homeostasis. Molecular Plant Microbe Interaction, 27(11), 1226-1240.
  31. Kuc, J. (2001). Concepts and direction of induced systemic resistance in plants and its application. European Journal of Plant Pathology, 107(1), 7-12.
  32. Lagrimini, L., Burkhart, M., Moyer, W. M. & Rothstein, S. (1987). Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: molecular analysis and tissue-specific expression. Proceedings of the National Academy of Sciences, 84(21), 7542-7546.
  33. Lavanya, S. N., Raj, S. N., Udayashankar, A. C., Kini, K. R., Amruthesh, K. N., Niranjana, S. R. & Shetty, H. S. (2012). Comparative analysis of activities of vital defence enzymes during induction of resistance in pearl millet against downy mildew. Archives Of Phytopathology And Plant Protection, 45(11), 1252-1272.
  34. Llorens, E., García-Agustín, P. & Lapeña, L. (2017). Advances in induced resistance by natural compounds: towards new options for woody crop protection. Scientia Agricola (Piracicaba, Braz.), 74(1), 90-100.
  35. Luna, E., Beardon, E., Ravnskov, S., Scholes, J. & Ton, J. (2015). Optimizing Chemically Induced Resistance in Tomato Against Botrytis cinerea. Plant Disease, 100(4), 704-710.
  36. Marcucci, E., Aleandri, M. P., Chilosi, G. & Magro, P. (2010). Induced resistance by β-aminobutyric acid in artichoke against white mould caused by Sclerotinia sclerotiorum. Journal of Phytopathology, 158(10), 659-667.
  37. Mayer, A. M. (2006). Polyphenol oxidases in plants and fungi: going places? A review. Phytochemistry 67(21), 2318-2331.
  38. Nandeeshkumar, P., Sudisha, J., Ramachandra, K. K., Prakash, H. S., Niranjana, S. R. & Shekar, Sh. H. (2008). Chitosan induced resistance to downy mildew in sunflower caused by Plasmopara halstedii. Physiological and Molecular Plant Pathology, 72, 188-194.
  39. Oka, Y., Cohen, Y. & Spiegel, Y. (1999). Local and systemic induced resistance to the root-knot nematode in tomato by DL-β-amino-n-butyric acid. Phytopathology, 89(12), 1138-1143.
  40. Papavizas, F. (1964). Greenhouse control of Aphanomyces root rot of peas with aminobutyric acid and methylaspartic acid. Plant Disease, 48, 537-541.
  41. Perazzolli, M., Dagostin, S., Ferrari, A., Elad, Y. & Pertot, I. (2008). Induction of systemic resistance against Plasmopara viticola in grapevine by Trichoderma harzianum T39 and benzothiadiazole. Biological Control, 47(2), 228-234.
  42. Raj, S. N., Sarosh, B. R. & Shetty, H. S. (2006). Induction and accumulation of polyphenol oxidaseactivities as implicated in development of resistance against pearl millet downy mildew disease. Plant Biology, 33, 563-571.
  43. Safdarpour, F., Khoda-karamian, Gh. & Soleymani-Pari, M. J. (2013). Induction of systemic resistance against blight disease of Cucumber Leaf margin using sodium silicate. Journal of Plant Protection, 36(1), 29-38. (in Farsi)
  44. Siegrist, J., Orober, M. & Buchenauer, H. (2000). β-Aminobutyric acid-mediated enhancement of resistance in tobacco to tobacco mosaic virus depends on the accumulation of salicylic acid. Physiological and Molecular Plant Pathology, 56(3), 95-106.
  45. Silué, D., Nashaat, N. & Tirilly, Y. (1996). Differential responses of Brassica oleracea and B. rapa accessions to seven isolates of Peronospora parasitica at the cotyledon stage. Plant Disease, 80(2), 142-144.
  46. Silue, D., Pajot, E. & Cohen, Y. (2002). Induction of resistance to downy mildew (Peronospora parasitica) in cauliflower by DL-β-amino-n-butanoic acid (BABA). Plant Pathology, 51(1), 97-102.
  47. Simmons, C. R. (1994). The physiology and molecular biology of plant 1, 3-β-D-glucanases and 1, 3; 1, 4-β-D-glucanases. Critical Reviews in Plant Sciences, 13(4), 325-387
  48. Slaughter, A. R., Hamiduzzaman, M. M., Gindro, K., Neuhaus, J. M. & Mauch-Mani, B. (2008). Beta-aminobutyric acid-induced resistance in grapevine against downy mildew: involvement of pterostilbene. European Journal of Plant Pathology, 122(1), 185-195.
  49. Ton, J. & Mauch-Mani, B. (2004). Beta-amino-butyric acid-induced resistance against necrotrophic pathogens is based on ABA-dependent priming for callose. Plant Journal, 38(1), 119-130
  50. Ton, J., Jakab G., Toquin, V., Flors, V., Iavicoli, A., Maeder, M. N., Mataux, J. P. & Mauch-Mani, B. (2005). Dissecting the β-aminobutyric acid–induced priming phenomenon in Arabidopsis. The Plant Cell, 17(3), 987-999.
  51. Wilkinson, S. W., Pastor, V., Paplauskas, S., Pétriacq, P. & Luna, E. (2018). Long-lasting β-aminobutyric acid-induced resistance protects tomato fruit against Botrytis cinerea. Plant Pathology, 67(1), 30-41.
  52. Yedidia, I., Benhamou, N., Kapulnik, Y. & Chet, I. (2000). Induction and accumulation of PR proteins activityduring early stages of root colonizationby the mycoparasite Trichoderma harzianum strain T-203. Plant Physiology and Biochemistry, 38(11), 863-873.
Iranian Journal of Plant Protection Science
Volume 49, Issue 1 - Serial Number 1
September 2018
Pages 143-154

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History

  • Receive Date: 22 November 2017
  • Revise Date: 21 February 2018
  • Accept Date: 27 February 2018

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