Progeny analysis of Iranian Citrus exocortis viroid isolate in two sensitive and tolerant rootstocks

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Plant Protection, Faculty of Agricultural Sciences & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor, Department of Plant Protection, Faculty of Agricultural Sciences & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Assistant Professor, Department of Plant breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources. Gorgan, Iran

4 Professor, Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

Abstract

Citrus exocortis viroid (CEVd) is the causal agent of exocortis disease, the economically important viroid disease of citrus. In this research, sequence polymorphism and population structure of CEVd were investigated in CEVd tolerant and sensitive hosts (sour orange (Citrus aurantium L.) and citrange (Poncirus trifoliata (L.) Raf. × C. sinensis (L.), respectively) seedlings. Full-length in vitro transcript of single sequence CEVd-S1 isolate was used for mechanical inoculation. The genetic diversity of CEVd populations was estimated in two citrus hosts by single-strand conformation polymorphism (SSCP) and sequencing. The analysis of cloned DNAs recovered from infected hosts by this isolate demonstrated that host species was effective on the variability within a single CEVd isolate. The amount and composition of the genetic diversity were different among the two hosts, and was higher in the tolerant host compared with the sensitive one. Furthermore, the analysis of thermodynamic secondary structures illustrated that nucleotide changes identified in this study did not induce major modifications in the viroid rod-like secondary structure.

Keywords

References

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Iranian Journal of Plant Protection Science
Volume 50, Issue 1
August 2019
Pages 1-13

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History

  • Receive Date: 15 July 2018
  • Revise Date: 05 September 2018
  • Accept Date: 22 November 2018

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