Effect of Cucumber mosaic virus and Cauliflower mosaic virus Infections on the Resistance of Canola to Turnip mosaic virus

Document Type : Research Paper

Authors

1 Former M. Sc. Student, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Associate Professor, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

Abstract

Turnip mosaic virus (TuMV) has frequently been reported from canola fields. Cultivation of resistant varieties is the best method to control viral diseases. However, plants that are resistant to a virus may lose their resistance in co-infection with other viruses. Therefore, in the present study the effect of mixed infection of TuMV with Cucumber mosaic virus (CMV) and Cauliflower mosaic virus (CaMV) on the resistance of a tolerant canola cultivar (Karaj 3) and a transgenic canola of RGS003spring variety with hairpin construct and high resistance to TuMV was investigated under greenhouse conditions. Canola plants were inoculated mechanically and then, relative TuMV content was assayed by semi-quantitative RT-PCR. Results demonstrated that mixed infection of TuMV and CaMV did not affect the resistance of transgenic canola plants against TuMV. Also, there was not any significant difference in TuMV content in the presence of CaMV. However, synergistic interaction between TuMV and CMV could overcome the resistance of canola plants to TuMV. The result of semi-quantitative RT-PCR indicated that TuMV accumulation was enhanced in the presence of CMV in transgenic plants, whereas TuMV levels were not affected by co-infection with CMV in Karaj 3. Therefore, it is concluded that for the introduction of TuMV-resistant transgenic plants, it is necessary to design a construct that the resulting transgenic plants would also be resistant to CMVsimultaneously.

Keywords

References

  1. Anderson, E. J., Qiu, S. G. & Schoelz, J. E. (1991). Genetic analysis of determinants of disease severity and virus concentration in cauliflower mosaic virus. Virology, 181(2), 647-655.
  2. Bance, V. B. (1991). Replication of potato virus X RNA is altered in coinfections with potato virus Y. Virology, 182(2), 486-494.
  3. Banuelos, G. S., Bryla, D. R. & Cook, C. G. (2002). Vegetative production of kenaf and canola under irrigation in central California. Industrial crops and products, 15(3), 237-245.
  4. Broadbent, L. (2015). Investigation of virus diseases of Brassica crops: Cambridge University Press, 112 pp.
  5. Ding, S. W., Anderson, B. J., Haase, H. R. & Symons, R. H. (1994). New overlapping gene encoded by the Cucumber mosaic virus genome. Virology, 198(2), 593-601.
  6. Dougherty, W., Lindbo, J., Smith, H., Parks, T., Swaney, S. & Proebsting, W. (1994). RNA-mediated virus resistance in transgenic plants: exploitation of a cellular pathway possibly involved in RNA degradation. Molecular Plant-Microbe Interactions, 7(5), 544-552.
  7. Doyle, J. J. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.
  8. Faria, J. C., Albino, M. M., Dias, B. B., Cançado, L. J., da Cunha, N. B., Silva, L. d. M., Vianna, G. R. & Aragao, F. J. (2006). Partial resistance to Bean golden mosaic virus in a transgenic common bean (Phaseolus vulgaris L.) line expressing a mutated rep gene. Plant Science, 171(5), 565-571.
  9. Garcia-Cano, E., Resende, R. O., Fernandez-Munoz, R. & Moriones, E. (2006). Synergistic interaction between Tomato chlorosis virus and Tomato spotted wilt virus results in breakdown of resistance in tomato. Phytopathology, 96(11), 1263-1269.
  10. Ghasemzadeh, A. & Shams-bakhsh, M. (2016). Reaction of canola cultivars to Cauliflower mosaic virus and co-infection of Turnip mosaic virus and Cauliflower mosaic virus under greenhouse condition. Plant Protection (Scientific Journal of Agriculture), 39(3), 71-82.‌ (in Farsi)
  11. Guerini, M. N. & Murphy, J. F. (1999). Resistance of Capsicum annuum ‘Avelar’ to pepper mottle potyvirus and alleviation of this resistance by co-infection with cucumber mosaic cucumovirus are associated with virus movement. Journal of General Virology, 80(10), 2785-2792.
  12. Guo, H. S. & Ding, S. W. (2002). A viral protein inhibits the long range signaling activity of the gene silencing signal. The European Molecular Biology Organization Journal, 21(3), 398-407.
  13. Hamilton, R. & Dodds, J. (1970). Infection of barley by tobacco mosaic virus in single and mixed infection. Virology, 42(1), 266-268.
  14. Hii, G., Pennington, R., Hartson, S., Taylor, C. D., Lartey, R., Williams, A., Lewis, D. & Melcher, U. (2002). Isolate-specific synergy in disease symptoms between cauliflower mosaic and turnip vein-clearing viruses. Archives of Virology, 147(7), 1371-1384.
  15. Jafari, M., Shams-bakhsh, M. & Moieni, A. (2016). Reaction of commercial canola varieties and lines against Turnip mosaic virus (TuMV) isolate. Iranian Journal of Plant Pathology, 52(2), 147-159.‌ (in Farsi)
  16. Jafari, M., Shams-bakhsh, M. & Moieni, A. (2018). Efficiency of the partial genome of Turnip mosaic virus (TuMV) to induce resistance in canola. Iranian journal of plant protection science, (in press). (in Farsi)
  17. Li, H. W., Lucy, A. P., Guo, H. S., Li, W. X., Ji, L. H., Wong, S. M. & Ding, S. W. (1999). Strong host resistance targeted against a viral suppressor of the plant gene silencing defence mechanism. The European Molecular Biology Organization Journal, 18(10), 2683-2691.
  18. Li, Y., Zhang, J., Zhao, F., Ren, H., Zhu, L., Xi, D. & Lin, H. (2016). The interaction between Turnip crinkle virus p38 and Cucumber mosaic virus 2b and its critical domains. Virus Research, 222, 94-105.
  19. Martín, S. & Elena, S. F. (2009). Application of game theory to the interaction between plant viruses during mixed infections. Journal of General Virology, 90(11), 2815-2820.
  20. Mitter, N., Sulistyowati, E. & Dietzgen, R. G. (2003). Cucumber mosaic virus infection transiently breaks dsRNA-induced transgenic immunity to Potato virus Y in tobacco. Molecular Plant-Microbe Interactions,16(10), 936-944.
  21. Mitter, N., Sulistyowati, E., Graham, M. W. & Dietzgen, R. G. (2001). Suppression of gene silencing: a threat to virus-resistant transgenic plants? Trends in Plant Science,6(6), 246-247.
  22. Nomura, K., Uekusa, H. & Kita, N. (2014). Suppression of transgene RNA silencing in transgenic Arabidopsis thaliana by a mild strain of Cucumber mosaic virus. Journal of General Plant Pathology,80(5), 443-448.
  23. Palukaitis, P. & García-Arenal, F. (2003). Cucumoviruses. Advances in Virus Research, 62, 241-323.
  24. Ryang, B. S., Kobori, T., Matsumoto, T., Kosaka, Y. & Ohki, S. T. (2004). Cucumber mosaic virus 2b protein compensates for restricted systemic spread of Potato virus Y in doubly infected tobacco. Journal of General Virology, 85(11), 3405-3414.
  25. Sanchez, F., Wang, X., Jenner, C., Walsh, J. & Ponz, F. (2003). Strains of Turnip mosaic potyvirus as defined by the molecular analysis of the coat protein gene of the virus. Virus Research, 94(1), 33-43.
  26. Sano, Y. & Kojima, M. (1989). Increase in cucumber mosaic virus concentration in Japanese radish plants co-infected with turnip mosaic virus. Japanese Journal of Phytopathology, 55(3), 296-302.
  27. Savenkov, E. & Valkonen, J. (2001). Coat protein gene-mediated resistance to Potato virus A in transgenic plants is suppressed following infection with another potyvirus. Journal of General Virology, 82(9), 2275-2278.
  28. Shahraeen, N., Farzadfar, S. & Lesemann, D. E. (2003). Incidence of viruses infecting winter oilseed rape (Brassica napus ssp. oleifera) in Iran. Journal of Phytopathology, 151(11‐12), 614-616.‌ (in Farsi)
  29. Siddiqui, S. A., Valkonen, J. P., Rajamäki, M. L. & Lehto, K. (2011). The 2b silencing suppressor of a mild strain of Cucumber mosaic virus alone is sufficient for synergistic interaction with Tobacco mosaic virus and induction of severe leaf malformation in 2b-transgenic tobacco plants. Molecular Plant-Microbe Interactions, 24(6), 685-693.
  30. Simon-Mateo, C., López-Moya, J. J., Guo, H. S., Gonzalez, E. & García, J. A. (2003). Suppressor activity of potyviral and cucumoviral infections in potyvirus-induced transgene silencing. Journal of General Virology, 84(10), 2877-2883.
  31. Smith, N. A., Singh, S. P., Wang, M. B., Stoutjesdijk, P. A., Green, A. G. & Waterhouse, P. M. (2000). Gene expression: total silencing by intron-spliced hairpin RNAs. Nature, 407(6802), 319-320.
  32. Syller, J. (2012). Facilitative and antagonistic interactions between plant viruses in mixed infections. Molecular Plant Pathology, 13(2), 204-216.
  33. Takeshita, M., Koizumi, E., Noguchi, M., Sueda, K., Shimura, H., Ishikawa, N., Matsuura, H., Ohshima, K., Natsuaki, T. & Kuwata, S. (2012). Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus. Molecular Plant-Microbe Interactions, 25(1), 18-27.
  34. Walsh, J. A. & Jenner, C. E. (2002). Turnip mosaic virus and the quest for durable resistance. Molecular Plant Pathology, 3(5), 289-300.
  35. Zahedi Tabarestani, A., Shams-bakhsh, M. & Safaei, N. (2010). Distribution of three important aphid borne canola viruses in Golestan province. Iranian journal of plant protection science, 41(2), 251-259.‌ (in Farsi)
Iranian Journal of Plant Protection Science
Volume 50, Issue 1
August 2019
Pages 119-127

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History

  • Receive Date: 12 December 2018
  • Revise Date: 01 March 2019
  • Accept Date: 13 April 2019

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