Mating type and fertility status in populations of Bipolaris sorokiniana, the causal agent of root and crown common rot of wheat in Iran

Document Type : Research Paper

Authors

1 Department of Plant Protection, College of Agriculture and Natural Resources, University of Zabol, Zabol, Iran.

2 Department of Plant Protection, College of Agriculture and Natural Resources, University of Zabol, Zabol, Iran

3 Assistant Prof. of Mycology, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran

4 Higher Education Center Shahid Bakeri Miyandoab, Urmia University, Iran.

5 Professor/Department of Plant Protection, College of Agriculture and Natural Science,, University of Tehran, Karaj, Iran

Abstract

Root and crown common rotdisease caused by Bipolaris sorokiniana, has caused severe losses of wheat products worldwide. A collection of 147 monoconidial isolates was made from cultivated wheat in West Azerbaijan (Miyandoab), Golestan (Gorgan), Alborz (Karaj), Kermanshah (Kermanshah) and Fars (Shiraz) provinces in 2017-2018. After morphological and molecular identification, mating type primers were designed based on a conserved DNA binding (alpha domain) in MAT-1 and the high mobility group (HMG) domain in MAT-2. Frequency of mating type idiomorphs of B. sorokiniana isolates were done by multiplex PCR. Among 147 isolates of five population, 86 isolates amplified by MAT-1 primer and 61 isolates by MAT-2 primer. Frequencies of MAT-1 alleles in Shiraz and Gorgan populations was more than the MAT-2 alleles. According to the results of the Chi-square test (X2), Karaj, Kermanshah and Miyandoab populations had not significant difference (P<0.05). Therefore, these three populations have the highest potential for sexual reproduction than the otherthreepopulations. In addition, isolates from all of the populations were studied to evaluate the sexual fertility in vitro. After two months, pseudothecia without mature asci and ascospores formed in media.

Keywords

References

  1. Abbas, K. I., Hameed, A. M and Jameel, M. S. 2008. Susceptibility of different cultivars of date palm Phoenix dactylifera L. to casualty of inflorescence rot in some Basrah regions. Basrah Journal of Date Palm Research, 7 (1), 61-70. (In Arabic).
    1. Abdullah, S.K., Asensio, L., Monfort, E., Gomez-Vidal, S., Palma-Guerrero, J., Salinas, J., Lopez-Llorca, L.V., Jansson, H. B. and Guarro, J. 2005. Occurrence in Elx, SE Spain of inflorescence rot disease of date palms caused by Mauginiella scaettae. Phytopathology, 153(7-8), 417–422.
    2. Al- Ahmar, M.A. 2009. An object-oriented expert system for diagnosis fungal disease of date palm. International Journal of Soft Computing, 4(5), 201-207.
    3. Alizadeh, A., Javan-Nikkhah, M., Zare, R., Fotouhifar, K. B., Damm, U. and Stukenbrock, E. H. 2015. New records of Colletotrichum species for the mycobiota of Iran. Mycologia Iranica, 2(2), 94–108.
    4. Alvanipour, H., Aminian, H., Alami-Saeid, K., Sorkheh, K., Farrokhinejad, R., Ali Nejati, A., and Javan-Nikkhah, M. (2020). Cross-transferability of SSR loci of Phaeosphaeria nodorum to Mauginiella scaettae. Mycologia Iranica, 7(1), 135-142.
    5. Al-Yaseri, I. I. and Ismail, A. Z. 2011. Efficacy of some fungicides to controlling date palm inflorescence rot caused by Mauginiella scaettae Cav. http://www. uokufa.edu.iq. (In Arabic).
    6. Baird, R. E., Phillip, A., Allen, T. W., McNeill, D., Wang, Z., Moulton, J. K., Rinehart, T. A., Abbas, H. K., Shier, T. and Trigiano, R. B. 2010. Variability of United States isolates of Macrophomina phaseolina based on simple sequence repeats and cross genus transferability to related genera within Botryosphaeriaceae. Mycopathologia, 170(3), 169-180.
    7. Bensaci, M. B., Rahmania, F. and Luis, A. J. M. 2015. The date palm inflorescence rot fungus Mauginiella scaettae can infects the model host Arabidopsis thaliana. 18th International Plant Protection Congress, 3-6 April., Berlin, pp. 299.
    8. Dowson, V. H. W. 1991. Date Production and Protection. (1st ed). Tehran. Ministry of Agriculture Extension Organization. (In Farsi).
    9. Djerbi, M. 1998. Disease of date palm: present status and future prospects. Journal of Agricultural and Marine Science, 3(1), 103-114.
    10. Dutech, C., Enjalbert, J., Fournier, E., Delmotte, F., Barre`s, B., Carlier, J., Tharreau, D. and Giraud, T. 2007. Challenges of microsatellite isolation in fungi. Fungal Genetics and Biology, 44(10), 933–949.
    11. Farrokhinejad, R. 1994. Investigation on the inflorescence rot of the date palm in Khuzestan. M.Sc. dissertation. Shahid Chamran University of Ahvaz. Khuzestan. (In Farsi).
    12. Fawcett, H. S. and Klotz, L. J. 1932. Diseases of the date palm, Phoenix dactylifera. Bulletin 522. University of California, Berkeley, College of agriculture. 47 PP.
    13. Galehdari, H., Foroughmand, A. M., Roshanfekr, H. and Nazari, M. 2005. Exhaustive Genetic Engineering. (1st ed). Qom. Golhaye Behesht. (In Farsi).
    14. Hameed, A. M. 2005. Susceptibility of different cultivars of Date palm (Phoenix dactylifera L.) to Mauginiella scaettae the causal agent of inflorescence rot. Basrah Journal for Date Palm Research, 4)1-2), 37-53. (In Arabic).
    15. Huang, L., Deng, Z., Li, R., Xia, Y., Bai, G., Siddique, K. H. M. and Guo, P. 2018. A fast silver staining protocol enabling simple and efficient detection of SSR markers using a non-denaturing polyacrylamide gel. Journal of Visualized Experiments, 134, 1-7.
    16. Jonoobi, M., Shafie, M., Shirmohammadli, Y., Ashori, A., Hosseinabadi, H. Z. and Mekonnen, T. 2019. A review on date palm tree: properties, characterization and its potential applications. Journal of Renewable Materials, 7(11), 1055-1075.
    17. Karaoglu, H., Lee, C. M. Y. and Meyer, W. 2005. Survey of simple sequence repeats in completed fungal genomes. Molecular Biology and Evolution, 22(3), 639-649.
    18. Kumar, S., Rai, S., Maurya, D. K., Kashyap, P. L., Srivastava, A. K. and Anandaraj, M. 2013. Cross-species transferability of microsatellite markers from Fusarium oxysporum for the assessment of genetic diversity in Fusarium udum. Phytoparasitica, 41(5), 615-622.
    19. Motallebi, M., Zamani, M. R. and Hoseinzadeh Kargar, A. 2002. Evaluation of the relationship between polygalacturonase activity and pathogenicity of Iranian isolates of Ascochyta rabiei, Journal of Hydrology and Soil Science, 6(4), 159-168. (In Farsi).
    20. Mohagery, A. R. 1995. Evaluation of reaction and persistency of date palm cultivars to inflorescence rot disease and yield loss in Khuzestan province. 12th Iranian Plant Protection Congress, September. Karaj, Iran. pp. 251. (In Farsi).
    21. Qayem, A. A. 2015. Middle Arabic - Persian Contemporary Culture (4th ed). Tehran. Farhang Moaser. (In Farsi).
    22. Rossetto, M. 2001. Sourcing of SSR markers from related plant species (Chap. 14). (pp. 211–224). Plant genotyping the DNA fingerprinting of plants. New York. CABI.
    23. Sheikholeslami, M., Okhovat, S. M., Hejaroude, Gh., Sharifi Tehrani, A., Javan Nikkhah, M. and Najafi Mirak, T. 2005. Investigation on interaction of isolate-genotype between Erysiphe betae and Beta vulgaris under greenhouse condition. Journal of sugar beet, 21(2), 123-135. (In Farsi).
    24. Sigler, L. and Carmichael, J. W. 1976. Taxonomy of Malbranchea and some other hyphomycetes with arthroconidia. Mycotaxon, 4, 349-488.
    25. Singh, R., Kumar, S., Lalkashyap, P., Kumar Srivastava, A., Mishra, S. and Kumar Sharma, A. 2014. Identification and characterization of microsatellite from Alternaria brassicicola to assess cross-species transferability and utility as a diagnostic marker. Molecular Biotechnology, 56(11), 1049–1059.
    26. Stukenbrock, E. H., banke, S., Zala, M., Mcdonald, B. A. and Oliver, R. P. 2005. Isolation and characterization of EST-derived microsatellite loci from the fungal wheat pathogen Phaeosphaeria nodorum. Molecular Ecology Notes, 5(4), 931–933.
    27. von Arx, J. A., van der Walt, J. and Liebenberg N. V. D. W. 1982. On Mauginiella scaettae. Sydowia, 34, 42–45.
    28. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innes, M. A., Gelfand, D. H., Sninsky, J. J., White, T. J. (eds) PCR protocols. Academic Press, London, pp 315–322.
    29. Zhong, S. and Steffenson, B. J. 2001. Virulence and molecular diversity in Cochliobolus sativus. Genetics and Resistance, 91(5), 469–476.
Iranian Journal of Plant Protection Science
Volume 52, Issue 2
February 2022
Pages 1-14

Files

History

  • Receive Date: 04 April 2021
  • Revise Date: 26 August 2021
  • Accept Date: 07 September 2021

Share

How to cite

Statistics