Evaluation of the Rouin1 biological fungicide in management of Rhizoctonia root rot of sugar beet

Document Type : Research Paper

Authors

1 Agricultural and natural resources research center of Kermanshah

2 Prof. Assistant for biological control Research Department, Iranian Research institute of Plant Protection, AREEO, Tehran, Iran.

3 Prof. Associate for Research Department of plant breeding, Sugar beet seed institute, Karaj, Iran.

4 Prof. Associate for Sugar beet Research Department, Kermanshah Agricultural and Natural Resources Research and Education Center, AREEO, Kermanshah, Iran

Abstract

In this study, the efficacy of the biological material named as Rouin1 based on Bacillus subtilis BS106 in controlling Rhizoctonia root rot disease of sugar beet was evaluated. The experiment was carried out as split plots in the pattern of randomized complete block design in two research farms in Kermanshah county. The major factor was seed coating with Rouin1 at various conditions. The minor factor was irrigating and not irrigating the plots with Rouin1 solution. All of the plants were inoculated with R. solani RS 124 inoculum 8 weeks after planting. The results of this study showed that major factor as seed coating had no effect on disease severity or crop yield in different forms, but minor factor as irrigating with bacterial solution reduced disease severity and increased crop yield at 1% probability level. By applying irrigation treatment of Rouin1 commercial material at the rate of 200 g/ha at two stages of 45 and 90 days after planting, the average disease index decreased from 4.4 (average control treatment) to 3.58 (average irrigation treatment). As a result, the disease index decreased by 0.82 units (approximately equivalent to 8.2% of the infection rate). Also, the average yield increased from 39,560 kg in the control treatment to 48080 in the irrigation treatment, which is equivalent to 17.72% of production increasing per hectare.

Keywords


  1. Asaka, O. & Shoda, M. (1996). Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Applied Environmental Microbiology, 62, 4081-4085.
  2. Behdad, E. (1980). Diseases of field crops in Iran. Ashrafi publications. Esfahan. Iran. 428p.
  3. Bhattacharyya, P. N. & Jha, D. K. (2012). Plant Growth Promoting Rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology, 28(4), 1327-1350. doi: 10.1007/s11274-011-0979-9.
  4. Brantner, J. R. & Windels, C. E. (1999). In furrow and postemergence application of Quadris for control of Rhizoctonia damping off and root and crown rot. Sugar beet Research and Extension Reports, 29, 275-277.
  5. Büttner, G., Pfähler, B. & Märländer, B. (2004). Greenhouse and field techniques for testing sugar beet for resistance to Rhizoctonia root and crown rot .Plant Breeding, 123, 158-166.
  6. Dunleavy, J. (1955). Control of damping-off of sugar beet by Bacillus subtilis. Phytopathology, 45, 252-258.
  7. El-Kazzaz, M. K., Badr, M. M., El-Zahaby, H. M. & Gouda, M. I. (2002). Biological control of seedling damping-off and root rot of sugar beet plants. Plant Protection Science, 38(2), 645–647.
  8. Fendrihan, S., Constantinescu, F., Sicuia, O. A. & Dinu, S. (2016). Beneficial Bacillus strains improve plant resistance to phytopathogens: a review. International Journal of Environment, Agriculture and Biotechnology, 1(2), 137-142.
  9. Fiddaman, P. J. & Rossal, S. (1993). The production of antifungal volatiles by Bacillus subtilis. Journal of Applied Bacteriology, 74, 119-126.
  10. Fiddaman, P. J. & Rossal, S. (1994). Effect of substrate on the production of antifungal volatiles from Bacillus subtilis. Journal of Applied Bacteriology, 76, 395-405.
  11. Gond, S. K., Bergen, M. S., Torres, M. S. & White J. J. F. (2015). Endophytic Bacillus spp. produce antifungal lipopeptides and induce host defense gene expression in maize. Microbiological Research, 172, 79-87.
  12. Harveson, R. M., Hein, G. L., Smith, J. A., Wilson, R. G. & Yonts, C. D. (2002). An integrated approach to cultivar evaluation and selection for increased profitability: a successful case study for the Central High Plains. Plant Disease, 86, 192-204.
  13. Hecker, R. J. & Ruppel, E. G. 1975. Inheritance of resistance to Rhizoctonia root rot in sugarbeet. Crop Science, 15, 487-490.
  14. Hussain, T. & Khan, A. A. (2019). Bacillus subtilis HussainT-AMU and its antifungal activity against potato black scurf caused by Rhizoctonia solani on tuber, Biocatalysis and Agricultural Biotechnology, doi: https://doi.org/10.1016/j.bcab.2019.101443
  15. Jamali, H., Sharma, A., Roohi & Srivasta, A. K. (2020). Biocontrol potential of Bacillus subtilis RH5 against sheath blight of rice caused by Rhizoctonia solani. Journal of Basic Microbiology, 60(3), 268-280.
  16. Karimi, E., Sadeghi, A., Abbaszade Dehaji, P., Dalvand, Y., Omidvari, M. & Kakouie nezhad, M. (2012). Biocontrol activity of salt tolerant Streptomyces isolates against phytopathogens causing root rot of sugar beet. Biocontrol Science and Technology, 22, 333-349
  17. Karimi, E, Safaie, N., Shams-Baksh, M. & Mahmoudi, S. B. (2016). Bacillus amyloliquefaciens SB14 from rhizosphere alleviates Rhizoctonia damping-off disease on sugar beet. Microbiological Research, 192, 221–230.
  18. Kajimora, Y., Svgiyama, M. & Kaneda, M. (1995). Bacillopeptins, new cyclic lipopeptide antibiotic from Bacillus subtilis FR-2. The Journal of Antibiotics, 48 (10), 1095-103.
  19. Kiewnick, S., Jacobsen, B. J., Braun-Kiewnick, A., Eckhoff, J. L. A. & Bergman, J. W. (2001). Integrated control of Rhizoctonia crown and root rot of sugar beet with fungicides and antagonistic bacteria. Plant Disease, 85, 718-722.
  20. Mahmoudi, S. B., Mesbah, M. & Alizadeh, A. (2004). Pathogenic variability of sugar beet isolates of Rhizoctonia solani. Iranian Journal of PlantPathology, 40 (3-4), 253-280.
  21. Mahmoudi, S. B. & Soltani, J. (2005). Sugar beet root rot in Iran. Newsletter of Training Center for Studying Sugar Industry in Iran, 178, 14-18.
  22. Mahmoud, A. F. (2016). Suppression of sugar beet damping-off caused by Rhizoctonia solani using bacterial and fungal antagonists. Archives of Phytopathology and Plant Protection, 49(19–20), 575–585. http://dx.doi.org/10.1080/03235408.2016.1245052.
  23. Narasimhan, A. & Shivakumar, S. (2016). Biocontrol of Rhizoctonia solani root rot of chilli by Bacillus subtilis formulations under pot conditions. Journal of Biological Control, 30(2), 109-118, doi: 10.18641/jbc/30/2/91995
  24. Neeta, L. & Manjusha, C. (2017). Production of Levansucrase from a soil isolate Bacillus subtilis (DQ922949). International Journal of Emerging Trends in Science and Technology, 4, 5714-5721.
  25. Rother, B. (1999). Situation of Rhizoctonia in Europe. International Institute for Sugar Beet Research Info, 4, 2-6.
  26. Qi, Y., Patra, G., Liang, X., Williams, L. E., Rose, S., Redkar, R. J. & DelVecchio, V. G. (2001). Utilization of the rpoB gene as a specific chromosomal marker for real-ime PCR detection of Bacillus anthracis. Applied and Environmental Microbiology, 67, 3720-3727.
  27. Shahiri Tabarestani, M., Falahati Rastegar, M., Jafarpour, B. & Rohani, H. (2004). Investigation of antagonistic effect of Bacillus subtilis on biological control of sugar beet damping-off disease. Sugar Beet, (20) 2, 161-175.
  28. Suslow, T. V. & Schroth, M. N. (1982). Rhizobacteria of sugar beets: Effects of seed application and root colonization on yield. Phytopathology, 72, 199-206.
  29. Windels, C. E., Kuznia, R. A. & Call, J. (1997). Characterization and pathogenicity of Thanatephorus cucumeris from sugar beet in Minnesota. Plant Disease, 81, 245-249.
  30. Whitney, E. D. & Duffus, J. E. (1986). Compendium of beet diseases and insects. APS Press. 76p.