Effect of developmental time on the cowpea weevil Callosobruchus maculatus adult longevity and mating behaviors

Document Type : Research Paper



This study investigated the effect of pre-maturity developmental time on adult longevity and mating behaviors of the cowpea weevil, Callosobruchus maculatus (Coleoptera: Bruchidae). Eggs of the same age and one-day-old virgin adult insects were used for the experiments. Longevity of adult insects associated with each developmental time, the number of eggs and hatching percentage, besides mating-related parameters, including mating success rate, males rejection rate, mating reluctance males, mating latency, onset time, and duration of kicking by the females and mating duration were recorded. The results showed that the length of the developmental time did not significantly affect the longevity of the female insects but showed a significant effect on the longevity of the male individuals. Male insects with a very short or very long developmental time had a shorter lifespan compared to the male insects that appeared in the middle of the period (with a medium developmental time). Male insects with longer developmental time had less ability to succeed against female insect resistance and eventually mating success. Copulation duration was also affected by the insect's developmental time length. In general, the cowpea weevil's developmental time directly impacts the longevity of adult insects, and their mating behaviors. In addition, attention to this issue is important in selecting insects for different experiments. It can play a significant role in reducing experimental errors.


  1. Amiri A., & Bandani A.R. (2021). Parents' living conditions influence offspring fitness and competency. Journal of Stored Products Research, 92 (101795).
  2. Andrés, J. & Cordero Rivera, A. (2000). Copulation duration and fertilization success in a damselfly: an example of cryptic female choice? Animal Behaviour, 59, 695-703.
  3. Araújo, M.dS., Gil, L.H.S. & e-Silva, A.dA. (2012). Larval food quantity affects development time, survival and adult biological traits that influence the vectorial capacity of Anopheles darlingiunder laboratory conditions. Malaria journal, 11, 
  4. Arnqvist, G. & Nilsson, T. (2000). The evolution of polyandry: multiple mating and female fitness in insects. Animal Behaviour, 60,145–164.
  5. Cope, J.M. & Fox, C.W. (2003). Oviposition Decisions in the Seed Beetle, Callosobruchus Maculatus (Coleoptera: Bruchidae): Effects of Seed Size on Superparasitism. Journal of stored products research, 39, 355-365.
  6. den Hollander, M. & Gwynne, D.T. (2009). Female fitness consequences of male harassment and copulation in seed beetles, Callosobruchus maculatus. Animal Behaviour, 78, 1061e1070.
  7. Eady, P.E., Brown, D.V. 2017. Male-female interactions drive the (un)repeatability of copula duration in an insect. Royal Society Open Science, 4, 160962.
  8. Edvardsson, M. & Canal, D. (2006). The effects of copulation duration in the bruchid beetle Callosobruchus maculatus. Behavioral Ecology, 17, 430–434.
  9. Fox, C.W. (1993). The influence of maternal age and mating frequency on egg size and offspring performance in Callosobruchus maculatus (Coleoptera: Bruchidae). Oecologia, 96(1),139-146. 
  10. Harrison, X.A. et al. (2018). A brief introduction to mixed effects modelling and multi-model inference in ecology. PeerJ 6, e4794.
  11. Lale, N.E.S. & Vidal, S. (2003). Effect of constant temperature and humidity on oviposition and development of Callosobruchus maculatus (F.) and Callosobruchus subinnotatus (Pic) on bambara groundnut, Vigna subterranea (L.) Verdcourt. Journal of Stored Products Research, 39, 459-70.
  12. Lang, B.J., Idugboe, S., McManus, K., Drury, F., Qureshi, A. & Cator, L.J. (2018). The Effect of Larval Diet on Adult Survival, Swarming Activity and Copulation Success in Male Aedes aegypti (Diptera: Culicidae). Journal of medical entomology, 55(1),29-35.
  13. Małek, D.K. & Czarnoleski, M. (2021). Thermal Preferences of Cowpea Seed Beetles (Callosobruchus maculatus): Effects of Sex and Nuptial Gift Transfers. Insects, 12, 310.
  14. Messina, F.J., Bloxham, A.J. & Seargent, A.J. (2007). Mating compatibility between geographic populations of the seed beetle Callosobruchus maculatus. Journal of Insect Behavior, 20, 489501.
  15. Omkar Singh, K. & Pervez, A.  (2006). Influence of mating duration on fecundity and fertility in two aphidophagous ladybirds. Journal of Applied Entomology, 130, 103–107.
  16. Rupesh S. et al. (2016). Growth and developmental responses of Callosobruchus maculatus (F.) on various pulses. Legume Research, (39),840-843.
  17. Simmons, L.W. (2001). Sperm competition and its evolutionary consequences in the insects. Princeton, New Jersey: Princeton University Press.
  18. van Lieshout, E., McNamara, K.B. & Simmons, L.W. (2014). Why Do Female Callosobruchus maculatus Kick Their Mates? PLoS One, 9(4), e95747.
  19. Vasudeva, R., Deeming, D.C. & Eady, P.E. (2018). Larval developmental temperature and ambient temperature affect copulation duration in a seed beetle. Behaviour, 155, 69–82.
  20. Wagner, J.D. & Bakare, A. (2017). Lifetime reproductive effort is equal between the sexes in seed beetles (Callosobruchus maculatus): dispelling the myth of the cheap male. Ethology Ecology and Evolution, 29, 387-396.
  21. Watanabe, N. (1990). Diversity in life cycle patterns of bruchids occurring in Japan (Coleoptera: Bruchidae): Bruchids and Legumes: Economics, Ecology and Coevolution. Kluwer Academic Publishers.
  22. Wolff, J. O. & Gorb, S. N. (2016). Biological Functions and Evolutionary Aspects: Attachment Structures and Adhesive Secretions in Arachnids. Biologically-Inspired Systems. Springer.