The pathogenicity of Beauveria bassiana IRAN 441C on onion thrips and its compatibility with ّّّّFlonicamid and Thiocyclam insecticides in laboratory conditions

Document Type : Research Paper

Authors

1 Plant Protection, Department of Plant Protection, Faculty of Agriculture, University of Zabol, P.O. Box: 98613-35856,, Zabol, Iran.

2 Department of Plant Protection, Faculty of Agriculture, University of Zabol, P.O. Box 98613-35856, Zabol, ran.

3 Department of Plant Protection, Faculty of Agriculture, University of Zabol, P.O. Box: 98613-35856,, Zabol Iran.

Abstract

Onion thrips is one of the most important pests of onion fields around the world, which reduces plant production. It is necessary to apply alternative strategies and reduce the concentration of chemical insecticides along with biological agents in order to prevent environmental pollution and insect resistance. Therefore, the objectives of this study were to evaluate the toxicity of Flonicamid and Thiocyclam, the virulence of entomopathogenic fungus Beauveria bassiana, and the efficiency of mixing fungus with insecticides on the 2nd instars of onion thrips and the biological factors of the pathogenic fungus. This study was performed as a completely randomized design with four replications under laboratory conditions. Insects in the control group were treated with distilled water. The results showed that LC50 values for Flonicamid, Thiocyclam and B. bassiana were 2.3 mgL-1, 4.3 mgL-1, and 5.4×107 spores mL-1, respectively. With increasing the concentration of insecticides and pathogenic fungus and also with increasing their application time, the mortality rate of onion thrips increased. Flonicamid had no effect on the germination and mycelium growth of the pathogenic fungus and was selected as a compatible insecticide. The results of mixing the pathogenic fungus with the studied insecticides showed that the studied pathogen at the concentration of 6×107 spore mL-1 along with Flonicamid at the concentration of 1.2 mgL-1 is the best combination to deal with onion thrips. Therefore, the above mixture can be recommended as an alternative for chemical insecticides after field studies in integrated management programs of onion thrips.

Keywords

References

  1. Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18, 265-267.
  2. Aguilar Carpio, C., González Rendón, A., Pérez Ramírez, A., Ramírez Rojas, S. G. & Carapia Ruiz, V. E. (2017). Chemical control of Thrips tabaci (Thysanoptera: Thripidae) in onion crop of Morelos, Mexico. Acta Zoológica Mexicana, 33(1), 39-44.
  3. Ain, Q., Mohsin, A. U., Naeem, M. & Shabbir, G. (2021). Effect of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, on Thrips tabaci Lindeman (Thysanoptera: Thripidae) populations in different onion cultivars. Egyptian Journal of Biological Pest Control, 31, 97.
  4. Ansari, M. A., Brownbridge, M., Shah, F. A. & Butt, T. M. (2008). Efficacy of entomopathogenic fungi against soil-dwelling life stages of western flower thrips, Frankliniella occidentalis, in plant-growing media. Entomologia Experimentalis et Applicata, 127(2), 80-87.
  5. Bilbo, T. R., Schoof, S. C. & Walgenbach, J. F. (2020). Foliar insecticide efficacy against western flower thrips in staked tomato, 2019. Arthropod Management Tests, 45(1), tsaa063.
  6. Boopathi, T., Pathak, K., Singh, B. & Verma, A. (2011). Efficacy of entomopathogenic fungi for the management of onion thrips, Thrips tabaci Lind. Pest Management In Horticultural Ecosystems, 17, 92-98.
  7. Dannon, H. F., Dannon, A. E., Douro-Kpindou, O. K., Zinsou, A. V., Houndete, A. T., Toffa-Mehinto, J., Elegbede, I. A. T. M., Olou, B. D. & Tamo, M. (2020). Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management. Journal of Cotton Research, 3, 24.
  8. Gulzar, S., Wakil, W. & Shapiro-Ilan, D. I. (2021). Combined effect of entomopathogens against Thrips tabaci Lindeman (Thysanoptera: Thripidae): Laboratory, greenhouse and field trials. Insects, 12(5), 456.
  9. Halder, J., Majumder, S. & Rai, A. (2021). Compatibility and combined efficacy of entomopathogenic fungi and neonicotinoid insecticides against Myzus persicae (Sulzer): An ecofriendly approach. Entomologia Hellenica, 30(1), 24-32.
  10. Hiremath, R., Ghante, V. N., Hosamani, A., leela, S. & Amaresh, Y. S. (2020). Combined effect of Beauveria bassiana (Balsamo) and selected chemical insecticides against sunflower whitefly Bemisia tabaci Gennadius. International Journal of Current Microbiology and Applied Sciences, 9(11), 3694-3704.
  11. Iglesias, L., Havey, M. J. & Nault, B. A. (2021). Management of onion thrips (Thrips tabaci) in organic onion production using multiple IPM tactics. Insects, 12(3), 207.
  12. Irigaray, F., Marco-Manceb, V. & PerezMoreno, I. (2003). The entomopathogenic fungus Beauveria bassiana and its compatibility with triflumuron: Effects on the two spotted spider mite Tetranychus urticae. Biological Control, 26(2), 168-173.
  13. Kachot, A. V., Jethva, D. M. & Patel, D. S. (2021). Laboratory efficacy of Beauveria bassiana (Balsamo) Vuillemin and chemical insecticide against onion thrips, Thrips tabaci Lindeman. The Pharma Innovation Journal, 10(9), 1456-1459.
  14. Kansagara, S., Shah, K. D., Rathod, A. R., Ghelani, M. K. & Acharya, M. F. (2018). Bio-efficacy of different insecticides against thrips (Scirtothrips dorsalis Hood) in green gram. Current Agriculture Research Journal, 6(3), 365-371.
  15. Li, D., Park, S. E., Lee, M. R., Kim, J. C., Lee, S. J. & Kim, J. S. (2021). Soil application of Beauveria bassiana JEF-350 granules to control melon thrips, thrips palmi Karny (Thysanoptera: Thripidae). Journal of Asia-Pacific Entomology, 24(3), 636-644.
  16. Maniania, N. K., Sithanantham, S., Ekesi, S., Ampong-Nyarko, K., Baumgärtner, J., Lohr, B. & Matoka, C. M. (2003). A field trial of the entomogenous fungus Metarhizium anisopliae for control of onion thrips, Thrips tabaci. Crop Protection, 22(3), 553-559.
  17. Martin, N., Workman, P. & Butler, R. (2003). Insecticide resistance in onion thrips (Thrips tabaci) (Thysanoptera: Thripidae). New Zealand Journal of Crop and Horticultural Science, 31(2), 99-106.
  18. Patel, D. S., Jethva, D. M. & Kchot, A. V. (2021). Evaluation of biopesticides and insecticidal spray schedule against Lipaphis erysimi (Kaltenbach). The Pharma Innovation Journal, 10(8), 164-167.
  19. Patel, D. S., Jethva, D. M. & Kchot, A. V. (2020). Compatibility studies of different insecticides with entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin. Journal of Entomology and Zoology Studies, 8(6), 2101-2104.
  20. Radosevich, D. L., Cloyd, R. A. Herrick, N. J. (2020). Effects of spray volume and application frequency on insecticide efficacy against adult western flower thrips (Frankliniella occidentalis) under greenhouse conditions. HortScience, 55(10), 1708-1714.
  21. Sain, S. K., Monga, D., Kumar, R., Nagrale, D. T., Hiremani, N. S. & Kranthi, S. (2019). Compatibility of entomopathogenic fungi with insecticides and their efficacy for IPM of Bemisia tabaci in cotton. Journal of Pesticide Science, 44(2), 97-105.
  22. Trisyono, A. & Whalon, M. E. (1999). Toxicity of neem applied alone and in combinations with Bacillus thuringiensis to Colorado potato beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 92, 1281-1288.
  23. Vineet, K. & Divender, G. (2019). Evaluation of insecticides against woolly apple aphid Eriosoma lanigerum and its parasitoid Aphelinus mali. Indian Journal of Entomology, 81(3), 467-471.
  24. Vincent, J. M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159, 850.
  25. Wari, D., Okada, R., Takagi, M., Yaguchi, M., Kashima, T. & Ogawara, T. (2020). Augmentation and compatibility of Beauveria bassiana with pesticides against different growth stages of Bemisia tabaci (Gennadius); an in vitro and field approach. Pest Management Science, 76(9), 3236-3252.
  26. Zhang, Z., Zheng, C., Keyhani, N. O., Gao, Y. & Wang, J. (2021). Infection of the western flower thrips, Frankliniella occidentalis, by the insect pathogenic fungus Beauveria bassiana. Agronomy, 11, 1910.
Iranian Journal of Plant Protection Science
Volume 53, Issue 1
April 2022
Pages 71-84

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History

  • Receive Date: 23 October 2021
  • Revise Date: 25 February 2022
  • Accept Date: 09 April 2022

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