Comparison of lethal and synergistic effects of abamectin and liquid sulfur on the fig spider mite, Eotetranychus hirsti (Acari: Tetranychidae)in vitro

Document Type : Research Paper


1 Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran.

2 Shiraz university


The fig spider mite, Eotetranychus hirsti Pritchard and Baker (Acari: Tetranychidae), is one of the most important pests of figs in Estahban city, which has the highest production of figs in Iran; Therefore, it is important to minimize the damage to this pest. It is important to choose the proper pesticide that has the highest mortality at the lowest possible concentration, has no adverse residue on the fig fruit, and has little environmental damage. In this regard, during 2016-2017, the acaricidal effect of abamectin, liquid sulfur, and a mixture of them was studied in order to determine LC50 and their synergistic effect in a completely randomized design including six treatments and four replications in vitro. This study was carried out at 28 ± 2°C, 55 ± 5 RH, and 16L:8D hours photoperiods. The results indicated a very strong synergistic effect in the application of a lethal concentration of zero percent liquid sulfur with very low amounts of abamectin, 0.06, 0.08, 0.1, 0.2 and 0.5 microliters per liter. Also, due to the significance of the LC50 ratio related to abamectin (The lack of number 1 in the confidencelimits), liquid sulfur, and the mixture of them, it is possible to understand the different effects of these compounds and due to the high mixing efficiency (reduction of 71.54 times in LD50).


  1. Baradaran, P., Arbabi, M., & Ranjbar, V. (2002). Comparative Population Fluctuation of Fig Spider Mite (Eotetranychus hirsti) on Fig Varieties in Saveh Region. Journal of Entomological Society of Iran, 22(1), 49-61.
  2. Baranowski, T. (1991). Avermectins-a new group of pesticides for plant protection. In: Research Session: Plant Protection, Poznan (Poland), 1991. Panstwowe Wydawnictwo Rolnicze i Lesne.
  3. Beers, E. H., Martinez-Rocha, L., Talley, R. R., & Dunley, J. E. (2009). Lethal, sublethal, and behavioral effects of sulfur-containing products in bioassays of three species of orchard mites. Journal of Economic Entomology, 102(1), 324-335.
  4. Boone, C., Bond, C., Hallman, A., & Jenkins, J. (2017). Sulfur General Fact Sheet. National Pesticide Information Center, Oregon State University Extension Services.
  5. Corbel, V., Raymond, M., Chandre, F., Darriet, F., & Hougard, J. M. (2004). Efficacy of insecticide mixtures against larvae of Culex quinquefasciatus (Say) (Diptera: Culicidae) resistant to pyrethroids and carbamates. Pest Management Science, 60(4), 375-380.
  6. Devonshire, A. L., & Rice, A. D. (1988). Aphid bioassay techniques. Their Biology, Natural Enemies and Control, 2, 119-128.
  7. Diczbalis,Y. (2018). Treatment for Mites on Lychee Fruit Prior to Irradiation for Improved Market Access. Final Report, Published and distributed by: Hort Innovation.
  8. Ebrahimi, L., & shiri, M. R. (2017). Lethal Effects of Abamectin and Propargite on Moghan Population of Two Spotted Spider Mite, Tetranychus urticae Koch (Acari, Tetranychidae), and Efficacy of Their Residual Effects on the Bean Plants. Journal of Applied Researches in Plant Protection, 6(3), 1-9.
  9. El Kady, G. A., El-Sharabasy, H. M., Mahmoud, M. F., & Bahgat, I. M. (2007). Toxicity of two potential bio-insecticides against moveable stages of Tetranychus urticae Journal of Applied Sciences Research. November, 1315-1319.
  10. Faghih, H., & Sabet Sarvestani, J. (2001). Fig: planting, cultivation and harvestin. Rahgosha Press, Shiraz, 292 pp.
  11. Gheibi, M., &, Taheri, Y. (2015). Effect of acaricide, Envidor speed, on figs spider mite, Eotetranychus hirsti (Acari: Tetranycidae). Plant Protection Journal, 6 (3), 211-223.
  12. Jafari, M., Zare, H., Golkar, G., Jokar, L., & Tabatabaei, Z. (2016). Evaluation of morphological characteristics in some fig (Ficus carica L.) genotypes. Seed and Plant Improvement Journal, 32(2) 147-163.
  13. Memarizadeh, N., Ghadamyari, M., Sajedi, R. H. & Jalali sendi, J. (2011). Mechanisms of Resistance to Abamectin in Two-Spotted Spider Mite, Tetranychus urticae Koch (Acari: Tetranychidae). Iranian Journal of Plant Protection Science, 42(1) 75-83.
  14. Paramasivam, M. & Selvi, C. (2017). Laboratory Bioassay Methods to Assess the Insecticide Toxicity Against Insect Pests-A Review. Journal of Entomology and Zoology Studies, 5(3): 1441-1445.
  15. Pritchard, A. E., & Baker, E. W. (1955). A Revision of the Spider Mite Family Tetranyohidae. San Francisco: Pacific Coast Entomological Society, 472 pp.
  16. Robertson, J. L., Preisler, H. K., & Russell, R. M. (2007a). PoloPlus: Probit and logit analysis user’s guide. LeOra Software, Petaluna, CA, USA.
  17. Robertson, J. L., Savin, N. E., Russell, R. M., & Preisler, H. K. (2007b). Bioassays with Arthropods, CRC Press. 224pp.
  18. Salahvarzi, a., Aliniaeifard, s. a., Abdali, n., & Ismaeili, a. (2010). Evaluation of nutritional and qualitative values of different fig cultivars in Lorestan province. Yafte, 11(5), 101-108.
  19. Talebi Jahromi, Kh. (2011). Pesticides Toxicology. University of Tehran Press, Tehran, 507 pp.