نوع مقاله : مقاله پژوهشی
نویسندگان
1 بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی بلوچستان، ایرانشهر، ایران. رایانامه
2 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت، ایران.
3 بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی کرمان، کرمان، ایران.
4 بخش تحقیقات بیماریهای گیاهی، موسسه تحقیقات گیاهپزشکی، تهران، ایران.
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
رThe provinces of Sistan and Baluchistan, along with the southern region of Kerman, are recognized as significant areas for producing tropical fruits. The advantageous climatic conditions prevalent in the southern part of the country, particularly in the aforementioned provinces, have contributed to the expansion of cultivated land dedicated to valuable tropical species such as Christ’s thorn jujube (Ziziphus spina-christi L.), Chico (Manilkara zapota L.), and Guava (Psidium guajava L.). The study aimed to isolate and identify fungi associated with die-back of some tropical fruit trees including Christ’s thorn jujube, Chico, and Guava, having die-back symptoms in Sistan and Baluchistan and Kerman Province. During 2022 and 2023, fungal isolation was done using a single spore and hyphal tip method. Seventy-one isolates with die-back symptoms were obtained from 150 samples and identified by morphological criteria. Finally, five fungal isolates with distinct morphological characteristics were selected for DNA extraction and nucleotide homology determination. To confirm the morphological identification, the ITS1-5.8S-ITS2 (ITS) and Elongation factor-1α (tef-1α) genomic regions were sequenced. Maximum Parsimony method for Lasiodiplodia isolates (ITS and tef-1α gene regions) and Neocosmospora isolates (tef-1α) in phylogeny analysis was used. The identified species in most locations included Neocosmospora solani on Christ’s thorn jujube, Chico and Guava, and Lasiodiplodia theobromae on Christ’s thorn jujube, which are reported for the first time in Iran.
کلیدواژه‌ها [English]
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