نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تهران، کرج- ایران
2 گروه علوم باغبانی، دانشکده کشاورزی ی، دانشگاه تهران ، کرج- ایران
3 گروه گیاهپزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
4 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تهران، کرج - ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Saffron (Crocus sativus L.), is one of the most valuable medicinal and spice plants globally, is significantly impacted by the Saffron latent virus (SaLV), a member of the Potyvirus genus (Potyviridae family). In Iran, SaLV infects over 70% of saffron plants in cultivation fields. Saffron is a rich source of apocarotenoids, including crocetin esters, picrocrocin, and safranal, which are crucial for its medicinal and culinary value This study evaluated two virus elimination methods- meristem culture (with meristem sizes of 0.3, 0.5, and 0.7 mm) and thermotherapy (at 50 ℃ for 60 min; at 40 ℃ for 60 min; and 28 ℃ for 21 days) - as well as their combination. SaLV presence was assessed using ELISA, RT-PCR, and RT-qPCR. The highest percentage of SaLV-free plants was achieved with a 0.3 mmmeristem culture (83.73% and 81.30%) and thermotherapy at 50 ° C for 60 min (71.36% and 66.36%), based on ELISA and RT-PCR, respectively.. The combined treatment of 0.3 mm meristem culture and thermotherapy at 50 °C for 60 min resulted in 100% SaLV-free plants, as confirmed by all three detection methods. This study provides an effective strategy for developing virus-free saffron cultivars, supporting sustainable saffron production and propagation.
Keywords: ELISA, RT-PCR, RT-qPCR, Virus elimination, saffron latent virus
کلیدواژه‌ها [English]
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