نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین المللی امام خمینی
2 مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.
3 مرکز تحقیقات زیست‌پزشکی (MBRC)، دانشگاه علوم پزشکی کرمانشاه
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
ABSTRACT
Rapeseed (Brassica napus L.) is widely cultivated oilseed crops in Iran and worldwide, valued for its role in producing edible oil, supporting energy production and contributing to food security. Studies have shown that Fusarium species causing crown and root rot diseases are among the major threats to canola production in Golestan Province and other regions of Iran. These fungal pathogens are responsible for considerable economic losses and reduction of canola yield. In this study, 103 contaminated rapeseed samples exhibiting crown and root rot symptoms were collected from canola-growing fields of Golestan Province. Among these samples, 79 Fusarium isolates were obtained, and the pathogenicity of 59 isolates was confirmed using pathogenicity assays. To identify the collected isolates at species-level, we used a combination of morphological characteristics and molecular/bioinformatics analyses using sequencing of the ITS and TEF-1α genes. Accordingly, the identified isolates were Fusarium acuminatum, Fusarium proliferatum, and Fusarium culmorum. Interestingly, Fusarium culmorum and Fusarium proliferatum were accounting for 47 and 42% of identified isolates among the collected samples. For the first time, this study report the pathogenicity of these three Fusarium isolates collected from the studied regions. The results highlights the importance of accurate identification and the integrated management of pathogenic Fusarium isolates to reduce economic losses and maintain healthy rapeseed production.
کلیدواژه‌ها [English]
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