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ویژگی‌های مولکولی و تبارزایی جدایه آفتابگردان ویروس موزائیک زرد لوبیا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ولی‌عصر(عج) ‏رفسنجان

2 استادیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ولی‌عصر(عج) رفسنجان

چکیده

ویروس موزائیک زرد لوبیا (Bean yellow mosaic virus, BYMV) از گونه‌های مهم جنس پوتی‌ویروس است که دارای گسترش جهانی می‌باشد.‌ در پژوهش حاضر ژنوم کامل یک جدایه ایرانی از این ویروس با نام اختصاری BYSun که برای اولین بار از آفتابگردان جداسازی شده است، توالی‌یابی شد و مورد بررسی قرار گرفت. برای تکثیر ژنوم تمام طول جدایه BYSun از سه راهکار کلی، بهره‌گیری از آغازگرهای دژنره و اختصاصی و تکثیر انتهای 5` با استفاده از روش 5` RACE استفاده گردید. اندازه آر ان ای ژنومی BYSun (بدون محاسبه دنباله (polyA 9547 نوکلئوتید تعیین شد که پلی­پروتئین بزرگی دارای 3056 اسیدآمینه را رمزگردانی می‌کند. در نواحی ترجمه‌نشدنی3'و5' به‌ترتیب 171 و 206 نوکلئوتید شناسایی شد. علاوه بر این، چارچوب خوانش PIPO نیز به‌صورت همپوشان در ناحیه P3 این جدایه مورد شناسایی قرار گرفت. بررسی با روش‌های مختلف در نرم‌افزار RDP4 نشان داد که جدایه BYSun دارای حداقل یک نوترکیبی در ژنوم خود می‌باشد. نتایج حاصل از مقایسه ترادف نوکلئوتیدی و اسیدآمینه‌ای ژنوم جدایه BYSun با ترادف‌ 23 جدایه موجود در بانک ژن نشان داد که جدایه استرالیایی BYMV-S که از باقلا جداسازی شده است بیشترین شباهت را با این جدایه دارد. در درخت‌های تبارزایی رسم شده بر اساس ترادف جدایه‌های مورد بررسی، جدایه‌های مختلف BYMV بسته به ناحیه ژنومی در شش تا هشت گروه قابل تفکیک هستند که جدایه BYSun به همراه جدایه استرالیایی در شاخه‌ای تحت عنوان S قرار می‌گیرند. جدایه BYSun اولین جدایه ایرانی ویروس موزائیک زرد لوبیا است که به صورت کامل تعیین ترادف شده است.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular and phylogenetic characteristics of sunflower isolate of Bean ‎yellow mosaic virus ‎

نویسندگان [English]

  • Mohammad Morad Moradi 1
  • Ahmad Hosseini 2
  • Samin Hosseini Farhangi 2
1 Former M.Sc. Student, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of ‎Rafsanjan, Rafsanjan, Iran
2 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Vali-e-Asr University of ‎Rafsanjan, Rafsanjan, Iran
چکیده [English]

Bean yellow mosaic virus (BYMV) is one of the most important members of the genus Potyvirus that is distributed worldwide. In the present study, the complete genome sequence and molecular characteristics of a BYMV sunflower isolate (BYSun) from Iran has been reported. The complete nucleotide sequence of BYSun was determined using degenerate and specific primers and 5`-RACE. The viral genome comprises 9547 nucleotides, excluding a 3´-terminal poly (A) sequence. The genome of BYSun has a 206 nt 5´-non coding and a 171 nt 3´-non coding region. The RNA encodes a single polyprotein of 3056 amino acid residues and has a deduced genome organization typical for the members of the genus Potyvirus and nine cleavage sites of the polyprotein were predicted. The small overlapping ORF (PIPO) in the P3 gene was also deduced. When analyzed using the RDP4 program, at least one recombination breakpoint was identified in BYSun genome. BYSun along with 23 full-length sequences of BYMV isolates from Genbank were subjected to phylogenetic analysis that showed the highest nucleotide and amino acid sequence identitywith Australian isolate of BYMV-S isolated from Broad bean. Phylogenetic analysis of different BYMV isolates sequences revealed existence of six to eight phylogenetic groupings depending on parts of investigated genome. BYSun and BYMV-S grouped apart from other isolates and were placed within a distinct group, currently designated “S”. This is the first report of a full-length sequence of a BYMV isolate from Iran.

کلیدواژه‌ها [English]

  • Bean yellow mosaic virus
  • full length genome sequence
  • Sunflower
  • phylogeny
Adams, M.J., Antoniw, J.F. & Beaudoin, F. (2005). Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Molecular Plant Pathology, 6, 471-487.
Chen, J., Chen, J. & Adams, M.J. (2001). A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Archives of Virology, 146, 757-766.
Chang, C., Hiebert, E. & Purcifull, D. E. (1988). Purification, characterization, and immunological analysis of nuclear inclusions induced by bean yellow mosaic and clover yellow vein potyviruses. Phytopathology, 78(10), 1266-1275.
Chu, P., Anderson, B., Khan, M., Shukla, D. & Higgins, T. (1999). Production of Bean yellow mosaic virus resistant subterranean clover (Trifolium subterraneum) plants by transformation with the virus coat protein gene. Annals of Applied Biology, 135, 469-480.
Chung, B. Y. W., Miller, W. A., Atkins, J. F. & Firth, A. E. (2008). An overlapping essential gene in the Potyviridae. Proceedings of the National Academy of Sciences, 105, 5897-5902.
Clark, M. F. & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475-483.
Dombrovsky, A., Huet, H., Chejanovsky, N. & Raccah, B. (2005). Aphid transmission of a potyvirus depends on suitability of the helper component and the N terminus of the coat protein. Archives of Virology, 150, 287-298.
Gallie, D. R. & Walbot, V. (1992). Identification of the motifs within the Tobacco mosaic virus 5′-leader responsible for enhancing translation. Nucleic Acids Research, 20, 4631-4638.
Gal-On, A. (2007). Zucchini yellow mosaic virus: insect transmission and pathogenicity. Molecular Plant Pathology, 8, 139-150.
Goetz, R., Huth, W., Lesemann, D.E. & Maiss, E. (2002). Molecular and serological relationships of Spartina mottle virus (SpMV) strains from Spartina spec. and from Cynodon dactylon to other members of the Potyviridae. Archives of Virology, 147, 379-391.
Guyatt, K. J., Proll, D. F., Menssen, A. & Davidson, A. D. (1996). The complete nucleotide sequence of bean yellow mosaic potyvirus RNA. Archives of Virology, 141(7), 1231-1246.
Ha, C., Coombs, S., Revill, P.A., Harding, R.M., Vu, M. & Dale, J. L. (2008). Design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. Archives of Virology, 153, 25-36.
Hammond, J. & Hammond, R. W. (2003). The complete nucleotide sequence of isolate BYMV-GDD of Bean yellow mosaic virus, and comparison to other potyviruses. Archives of Virology, 148(12), 2461-2470.
Kaur, C., Raj, R., Srivastava, A., Kumar, S. & Raj, S.K. (2018). Sequence analysis of six full-length bean yellow mosaic virus genomes reveals phylogenetic diversity in India strains, suggesting subdivision of phylogenetic group-IV. Archives of Virology, 163, 235-242.
Kehoe, M. A., Coutts, B. A., Buirchell, B. J. & Jones, R. A. (2014). Plant virology and next generation sequencing: experiences with a Potyvirus. PLoS ONE, 9(8), e104580.
Parrella, G. & Lanave, C. (2009). Identification of a new pathotype of Bean yellow mosaic virus (BYMV) infecting blue passion flower and some evolutionary characteristics of BYMV. Archives of Virology, 154, 1689-1694.
Rabiee, S. (2013). Distribution and characterization of most prevalent potyviruses in sunflower fields of Isfahan province. M.Sc. Thesis in Plant Pathology, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran (in Farsi with English abstract).
Riechmann, J.L., Lain, S. & Garcia, J.A. (1992). Highlights and prospects of potyvirus molecular biology. Journal of General Virology, 73, 1-16.
Sasaya, T., Shimizu, T., Nozu, Y., Nishiguchi, M., Inouye, N. & Koganezawa, H. (1997). Biological, serological, and molecular variabilities of clover yellow vein virus. Phytopathology, 87(10), 1014-1019.
Shukla, D. D., Ward, C. W. & Brunt, A. A. (1994). The Potyviridae. Wallingford, UK, CAB International.
Wylie, S., Coutts, B., Jones, M. & Jones, R. (2008). Phylogenetic analysis of Bean yellow mosaic virus isolates from four continents: relationship between the seven groups found and their hosts and origins. Plant Disease, 92, 1596-1603.
Wylie, S. & Jones, R. (2009). Role of recombination in the evolution of host specialization within Bean yellow mosaic virus. Phytopathology, 99, 512-518.
Xu, D. L., Zhou, G. H., Xie, Y. J., Mock, R. & Li, R. (2010). Complete nucleotide sequence and taxonomy of Sugarcane streak mosaic virus, member of a novel genus in the family Potyviridae. Virus Genes, 40, 432-439.
Zhou, Y., Mammen, M.P., Klungthong, C., Chinnawirotpisan, P., Vaughn, D.W., Nimmannitya, S., Kalayanarooj, S., Holmes, E.C. & Zhang, C. (2006). Comparative analysis reveals no consistent association between the secondary structure of the 3'-untranslated region of dengue viruses and disease syndrome. Journal of General Virology, 87, 2595-2603.