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ایدیومورف‌های تیپ آمیزشی و وضعیت باروری جنسی جدایه‌های Fusarium proliferatum حاصل از برخی اکوسیستم‌های آبی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه‌پزشکی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، 77871-31587، ایران

2 دانشیار گروه گیاه‌پزشکی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، 77871-31587، ایران

3 مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

چکیده

در این تحقیق برای شناسایی ایدیومورف های تیپ آمیزشی و باروری جنسی، از برخی رودخانه‌ها، دریاچه‌ها، تالاب‌ها و سدهای واقع در 10 استان (آذربایجان‌شرقی، البرز، بوشهر، خراسان جنوبی، خوزستان، فارس، کردستان، گیلان، لرستان و مازندران) نمونه برداری شد. در هر محل، نمونه ها از سه بستر آب، رسوبات و کف (حباب‌های ریز روی آب) اخذ شدند. برای جداسازی جدایه‌ها از محیط‌کشت آب-آگار حاوی آنتی‌بیوتیک و دمای 25 درجه سلسیوس و نور متناوب و محیط‌کشت پپتون پی سی ان بی آگار حاوی آنتی‌بیوتیک استفاده شد. تعداد 51 جدایه از F. proliferatum به دست آمد. شناسایی ریخت‌شناختی روی محیط کشت‌های برگ میخک-آگار، سیب‌زمینی-دکستروز-آگار و SNA انجام گرفت. سپس جدایه‌ها با استفاده از آغازگرهای اختصاصی گونه شناسایی شدند. برای تعیین ایدیومورف‌های تیپ آمیزشی از آغازگرهای طراحی شده بر اساس جایگاه‌های ژنی MAT در آزمون multiplex PCR استفاده شد. از 51 جدایه، تیپ آمیزشی 28 جدایه (9/54 درصد) از نوع MAT-1 و 23 جدایه (1/45 درصد) از نوع MAT-2 تشخیص داده شد. به منظور بررسی وضعیت باروری جنسی، جدایه-های یک تیپ آمیزشی با تمام جدایه‌های تیپ آمیزشی مخالف روی محیط‌کشت هویج-آگار تلاقی داده شدند. در مجموع 1288 تلاقی بین جدایه‌ها انجام گرفت. در نتیجه، در 102 تلاقی پریتسیوم‌های بارور مشاهده شدند که آسکوسپورهای تولید شده در آن‌ها 70 تا 80 درصد قابلیت جوانه‌زنی داشتند. پریتسیوم‌ها در تلاقی‌های چهار جدایه ماده بارور با جدایه‌های تیپ آمیزشی مخالف تولید شدند. فراوانی ایدیومورف‌های تیپ آمیزشی و وضعیت باروری جنسی جدایه‌های مورد بررسی، تایید کننده احتمال وقوع تولیدمثل جنسی بین جدایه‌های این گونه در اکوسیستم های آبی می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Mating type idiomorphs and sexual fertility status in Fusarium proliferatum isolates of some aquatic ecosystems

نویسندگان [English]

  • Razieh Poursaeid 1
  • Khalil-Berdi Fotouhifar 2
  • Rasoul Zare 3
1 Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
2 Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
3 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
چکیده [English]

In this study, in order to identification of mating type idiomorphs and possibility of sexual fertility samples were taken from rivers, lakes, lagoons and dams of 10 provinces (Alborz, Bushehr, East Azerbaijan, Fars, Guilan, Khuzestan, Kurdistan, Lorestan, Mazandaran and South Khorasan). In each site, samples were taken from three substrates of water, sediments and foam (small bubbles on water surface). Water-agar containing antibiotic and alternating light in 25°C and peptone-PCNB-agar containing antibiotic were used for isolation of the isolates. Fifty-one isolates of F. proliferatum were recovered and maintained. Isolates were identified using morphological characteristics on carnation-leaf-agar, potato dextrose agar and SNA culture media. Then, identification of isolates was done by species-specific primers. To detect the mating type idiomorphs of the isolates, idiomorph loci were amplified by multiplex PCR using the primers designed based on corresponding MAT loci. Of 51 isolates, 28 (54.9%) were determined as MAT-1 and 23 (45.1%) as MAT-2. To test sexual fertility of the isolates, each isolate was crossed with all isolates of opposite mating type on carrot agar culture medium, and total crosses of 1288 were done between the isolates. One hundred and two crosses resulted in fertile perithecia and ascospores recovered from fertile crosses had germination rate between 70–80%. Among successful crosses, four isolates were identified as female fertile. Distribution of mating type idiomorphs and fertility status of the isolates, confirms the probability of sexual reproduction among isolates in the aquatic ecosystems.

کلیدواژه‌ها [English]

  • Sexual reproduction
  • Female fertile
  • Male fertile
  • river
  • Lagoon
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