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فیتوهورمون اسپرمیدین و القا مقاومت دو رقم متحمل ترمه و حساس کاپیتان گوجه فرنگی به بیماری پژمردگی فوزاریومی ناشی از Fusarium oxysporum fsp. Lycopersici

نوع مقاله : مقاله پژوهشی

نویسندگان

1 هیات علمی دانشگاه یزد

2 گروه زراعت دانشگاه پیام نور یزد

3 استادیار فیزیولوژی گیاهی گروه زیست شناسی دانشکده علوم دانشگاه یزد

4 دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی دانشکده منابع طبیعی و کویر شناسی دانشگاه یزد

5 کارشناس آزمایشگاه گروه زیست شناسی دانشکده علوم دانشگاه یزد

چکیده

در این تحقیق اثر پلی آمین اسپرمیدین در القا مقاومت گیاه گوجه فرنگی به این بیماری پژمردگی فوزاریمی ناشی از قارچ Fusarium oxysporum fsp. lycopercisi در دو رقم حساس کاپیتان و متحل ترمه گوجه فرنگی مورد ارزیابی قرار گرفت. بدین منظور یک طرح فاکتوریل بر پایه طرح بلوک های کاملاً تصادفی در شرایط گلخانه انجام گردید. محلول اسپرمیدین با غلظتهای 1 و 1/0 میلی مولار با روش مه پاشی گیاهان آلوده به بیماری و سالم در مرحله چهار برگی مورد استفاده قرار گرفت. نمونه برداری در دو بازه زمانی ( 48 و 72 ساعت) بعد از آلودگی انجام گرفت. میزان چند آنزیم آنتی اکسیدانی و همچنین بیان تعدادی از فاکتورهای نسخه برداری مرتبط با بیماری زایی (npr1, eds1, pds) به ترتیب با روش طیف سنجی و بیان ژن در زمان واقعی اندازه گیری شدند. نتایج نشان داد که تقریباً بیشترین میزان فعالیت آنزیمی مرتبط با گیاهان گوجه فرنگی آلوده با تیمار 1 میلی مولار اسپرمیدین بعد از 72 ساعت از بیماری زایی به وسیله قارچ fusarium oxysporum fsp. lycopercisi می باشد. آنالیز بیان ژن نشان داد که تمام ژن های مورد بررسی در گیاهان بیمار، تحت تاثیر کاربرد اسپرمیدین قرار گرفته اند. بیشترین و کمترین میزان بیان به ترتیب مربوط به ژن های npr1 و pds بود. بر اساس این نتایج ما پیشنهاد می کنیم که کاربرد خارجی اسپرمیدین با غلظت مناسب در گیاهان تحت تنش می تواند شرایط رشدی گیاه را بهبود بخشیده که این امر می تواند منجر به مقاومت گیاهان به حمله بیمارگر شود.

کلیدواژه‌ها


عنوان مقاله [English]

Spermidin phytohormone and induce resistance of two tolerant Termeh and sensitive Capitan cultivar of tomato against Fusarium wilting disease caused by Fusarium oxysporum fsp. Lycopersici

نویسندگان [English]

  • Seyed kazem Sabbagh 1
  • Masoud Golestani 2
  • Mohammad Reza Sarafaraz 3
  • Esmaeil Abbasi 4
  • Marziyeh Taheri 5
1 ِDepartment of Biology, Faculty of Science, Yazd University
2 Dep of Agriculture Payamnoor university of Yazd
3 epartment of Biology, Faculty of Science, Yazd University
4 MSc student of Biotechnology, Department of Biotechnology, Faculty of Natural Resources of Desert, Yazd University
5 1Department of Biology, Faculty of Science, Yazd University
چکیده [English]

In this research, the effects of spermidin polyamine on induce resistance of tomato to fusarium wilting disease caused by Fusarium oxysporum fsp. Lycopercisi was assayed. For this reason, a designed factorial experimental based on randomized completed block design in greenhouse condition was done. A solution of spermidin at 1 and 0.1 mM concentration was used by the spray on infected and non infected plants at 4 leaf stage. Sampling was performed at two time interval (48 and 72h) after inoculation. The level of several antioxidants enzyme and also expression of some transcription factors as related to pathogenesis (npr1, eds1 and pds genes) were measured by spectophotometery and real time PCR, respectively. The results showed that approximately, the highest enzyme activity was related to infected tomato plants treated with 1mM spermidin after 72h post inoculation with fusarium oxysporum fsp. Lycopercisi. Gene expression analysis showed that all tested genes were affected by spermidin application in infected plants. The highest and lowest expression level was observed for npr1 and pds genes, respectively. Based on these results we suggest that exogenous application of sprmidin with appropriate concentration to plant under stress condition could improve plant growth condition which can be resulted to plant resistance against pathogen attack

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • gene expression
  • Induced resistance
  • Plant disease
  • Spermidin
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