فیتوهورمون اسپرمیدین و القا مقاومت دو رقم متحمل ترمه و حساس کاپیتان گوجه فرنگی به بیماری پژمردگی فوزاریومی ناشی از Fusarium oxysporum fsp. Lycopersici

نوع مقاله : مقاله پژوهشی


1 هیات علمی دانشگاه یزد

2 گروه زراعت دانشگاه پیام نور یزد

3 استادیار فیزیولوژی گیاهی گروه زیست شناسی دانشکده علوم دانشگاه یزد

4 دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی دانشکده منابع طبیعی و کویر شناسی دانشگاه یزد

5 کارشناس آزمایشگاه گروه زیست شناسی دانشکده علوم دانشگاه یزد


در این تحقیق اثر پلی آمین اسپرمیدین در القا مقاومت گیاه گوجه فرنگی به این بیماری پژمردگی فوزاریمی ناشی از قارچ Fusarium oxysporum fsp. lycopercisi در دو رقم حساس کاپیتان و متحل ترمه گوجه فرنگی مورد ارزیابی قرار گرفت. بدین منظور یک طرح فاکتوریل بر پایه طرح بلوک های کاملاً تصادفی در شرایط گلخانه انجام گردید. محلول اسپرمیدین با غلظتهای 1 و 1/0 میلی مولار با روش مه پاشی گیاهان آلوده به بیماری و سالم در مرحله چهار برگی مورد استفاده قرار گرفت. نمونه برداری در دو بازه زمانی ( 48 و 72 ساعت) بعد از آلودگی انجام گرفت. میزان چند آنزیم آنتی اکسیدانی و همچنین بیان تعدادی از فاکتورهای نسخه برداری مرتبط با بیماری زایی (npr1, eds1, pds) به ترتیب با روش طیف سنجی و بیان ژن در زمان واقعی اندازه گیری شدند. نتایج نشان داد که تقریباً بیشترین میزان فعالیت آنزیمی مرتبط با گیاهان گوجه فرنگی آلوده با تیمار 1 میلی مولار اسپرمیدین بعد از 72 ساعت از بیماری زایی به وسیله قارچ fusarium oxysporum fsp. lycopercisi می باشد. آنالیز بیان ژن نشان داد که تمام ژن های مورد بررسی در گیاهان بیمار، تحت تاثیر کاربرد اسپرمیدین قرار گرفته اند. بیشترین و کمترین میزان بیان به ترتیب مربوط به ژن های npr1 و pds بود. بر اساس این نتایج ما پیشنهاد می کنیم که کاربرد خارجی اسپرمیدین با غلظت مناسب در گیاهان تحت تنش می تواند شرایط رشدی گیاه را بهبود بخشیده که این امر می تواند منجر به مقاومت گیاهان به حمله بیمارگر شود.


عنوان مقاله [English]

Spermidin phytohormone and induce resistance of two tolerant Termeh and sensitive Capitan cultivar of tomato against Fusarium wilting disease caused by Fusarium oxysporum fsp. Lycopersici

نویسندگان [English]

  • Seyed kazem Sabbagh 1
  • Masoud Golestani 2
  • Mohammad Reza Sarafaraz 3
  • Esmaeil Abbasi 4
  • Marziyeh Taheri 5
1 ِDepartment of Biology, Faculty of Science, Yazd University
2 Dep of Agriculture Payamnoor university of Yazd
3 epartment of Biology, Faculty of Science, Yazd University
4 MSc student of Biotechnology, Department of Biotechnology, Faculty of Natural Resources of Desert, Yazd University
5 1Department of Biology, Faculty of Science, Yazd University
چکیده [English]

In this research, the effects of spermidin polyamine on induce resistance of tomato to fusarium wilting disease caused by Fusarium oxysporum fsp. Lycopercisi was assayed. For this reason, a designed factorial experimental based on randomized completed block design in greenhouse condition was done. A solution of spermidin at 1 and 0.1 mM concentration was used by the spray on infected and non infected plants at 4 leaf stage. Sampling was performed at two time interval (48 and 72h) after inoculation. The level of several antioxidants enzyme and also expression of some transcription factors as related to pathogenesis (npr1, eds1 and pds genes) were measured by spectophotometery and real time PCR, respectively. The results showed that approximately, the highest enzyme activity was related to infected tomato plants treated with 1mM spermidin after 72h post inoculation with fusarium oxysporum fsp. Lycopercisi. Gene expression analysis showed that all tested genes were affected by spermidin application in infected plants. The highest and lowest expression level was observed for npr1 and pds genes, respectively. Based on these results we suggest that exogenous application of sprmidin with appropriate concentration to plant under stress condition could improve plant growth condition which can be resulted to plant resistance against pathogen attack

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • gene expression
  • Induced resistance
  • Plant disease
  • Spermidin
Amini, J. & Sidovich, D. (2010). The effects of fungicides on Fusarium oxysporum f sp. lycopersici associated with Fusarium wilt of tomato. Journal of Plant Protection Research, 50(2), 172-178. (In Farsi)
2. Bolok Yazdi, H. R., Sabbagh, S. K., Mazaheri, M., Salar, M. & Moshtaghioun, S. M. (2018). Virus-induced gene silencing for functional analysis of eds1 gene in tomato infected with Ralstonia solanacearum. Zemdirbyste-Agriculture, 105(4), 357-362
3. Fraser, P.D., Truesdale, M.R., Bird, C.R., Schuch, W. & Bramley, P.M. (1994). Carotenoid biosynthesis during tomato fruit development (evidence for tissue-specific gene expression). Plant Physiology, 105(1), 405-413.
4. Fravel, D., Olivain, C. & Alabouvette, C. (2003). Fusarium oxysporum and its biocontrol. New Phytologist, 157(3), 93-502  
5. García-Fraile, P., Menéndez, E., Celador-Lera, L., Díez-Méndez, A., Jiménez-Gómez, A., Marcos-García, M. & Rivas, R. (2017). Bacterial probiotics: a truly green revolution. Probiotics and Plant Health, 131-162
 6. Holl,   J.M., Bianchi, F.J., Entling, M.H., Moonen, A.C., Smith, B.M. & Jeanneret, P. (2016). Structure, function and management of semi‐natural habitats for conservation biological control: a review of European studies. Pest Management Science, 72(9), 1638-1652
7. Huang, J., Liu, J., Li, Y. & Chen, F. (2008). Solution & characterization of the phyton desaturase gene as apotential selective marker for genetic enginering of the astaxanthin production green algae Chlorella zofingiensis. Journal of Phycology, 44(3), 684-690.
Jebara, S., Jebaram M., Limamm F., Aouanim ME. (2005). Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. Journal of Plant Physiology,162(8), 929-36
8. Kim, N.H., Kim, B. S. & Hwang, B.K. (2013). Pepper arginine decarboxylase is required for polyamine and γ-aminobutyric acid signaling in cell death and defense response. Plant Physiology, 162(4), 2067-2083.
9. Kusano, T., Yamaguchi, K., Berberich, T. & Takahashi, Y. (2007). Advances in polyamine research in 2007. Journal of Plant Research, 120(3), 345-350.
10. Liu, Y., Schiff, M., Marathe, R. & DineshKumar, S. (2002). Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N‐mediated resistance to tobacco mosaic virus. The Plant Journal, 30(4), 415-429.
11. Lois, L.M., Rodríguez‐Concepción, M., Gallego, F., Campos, N. & Boronat, A. (2000). Carotenoid biosynthesis during tomato fruit development: regulatory role of 1‐deoxy‐D‐xylulose 5‐phosphate synthase. The Plant Journal, 22(6), 503-513.
12. Mandal, S., Mallick, N. & Mitra, A. (2009). Salicylic acid-induced resistance to Fusarium oxysporum f. sp. lycopersici in tomato. Plant Physiology and Biochemistry, 47(7), 642-649
13. Mishra, S., Singh, A., Keswani, C., Saxena, A., Sarma, B. & Singh, H. (2015). Harnessing plant-microbe interactions for enhanced protection against phytopathogens Plant Microbes Symbiosis: Applied Facets, 111-125
14. Orzaez, D., Mirabel, S., Wieland , W.H. & Granell, A. (2006). Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit. Plant Physiology, 140(1), 3-11.
15. Pathak, D., Kumar, M. & Rani, K. (2017). Biofertilizer application in horticultural crops. In: Deepak, P., Ygeshvari, K.J., Vyas, R. & Shelat, H. (Ed.). Microorganisms for Green Revolution: Microb for sustainable crop protection,1, 215-227).
16. Prabhavathi, V.R. & Rajam, M.V (2007). Polyamine accumulation in transgenic eggplant enhances tolerance to multiple abiotic stresses and fungal resistance. Plant Biotechnology, 24(3), 273-282.
17. Rajam, M. V., Weinstein, L. H. & Galston, A. W. (1985). Prevention of a plant disease by specific inhibition of fungal polyamine biosynthesis. Proceedings of the National Academy of Sciences, 82(20), 6874-6878.
18. Sabbagh, E., Sabbagh, S.K., Panjehkeh, N. & Bolok-Yazdi, H.R. (2018). Jasmonic Acid Induced Systemic Resistance in Infected Cucumber by Pythium aphanidermatum. Tarim Journal of Agricultural Sciences, 24(1), 143-152.
19. Sabbagh, S.K., Kermanizadeh, B., Gholamalizadeh, A. & Sirousmehr, A. (2016). Effects of fertilizer treatments on components, performance components and induce resistance to wheat scab disease. Iranian Journal of Filed Crop Science, 47(1), 77-85 .(In Farsi)
20. Sabbagh, S.K., Poorabdollah, A., Sirousmehr, A. & Gholamalizadeh, A. (2017). Bi-fertilizers and Systemic Acquired Resistance in Fusarium Infected Wheat. Journal of Agricultural Science and Technology, 19(2), 453-464
21. Sabbagh, S.K., Roudini, M. & Panjehkeh, N. (2017). Systemic resistance induced by Trichoderma harzianum and Glomus mossea on cucumber damping-off disease caused by Phytophthora melonis. Archives of Phytopathology and Plant Protection, 50(7-8), 375-388. .(In Farsi)
22. Sabbagh, S.K., Kermanizadeh, B., Gholamalizzadeh Ahangar, A. & Sirousmehr, A. (2016). Effects of fertilizer treatments on components, performance components and induce resistance to wheat scab disease. Iranian Journal of Field Crop Science, 1(47), 77-85. (In Farsi)
23. Sarma, B.K., Yadav, S.K., Singh, S. & Singh, H. B. (2015). Microbial consortium-mediated plant defense against phytopathogens: readdressing for enhancing efficacy. Soil Biology and Biochemistry, 87, 25-33.
24. Seevers, P., Daly, J. & Catedral, F. (1971). The role of peroxidase isozymes in resistance to wheat stem rust disease. Plant Physiology, 48(3), 353-360.
25. Takahashi, Y. (2016). The role of polyamines in plant disease resistance. Environmental Control in Biology, 54(1), 17-21.
26. Villanueva-Alonzo, H.J., Us-Camas, R.Y., López-Ochoa, L. A., Robertson, D., Guerra-Peraza, O., Minero-García, Y. & Moreno-Valenzuela, O.A. (2013). A new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim. Biotechnology Letters, 35(5), 811-823.
27. Waie, B. & Rajam, M.V. (2003). Effect of increased polyamine biosynthesis on stress responses in transgenic tobacco by introduction of human S-adenosylmethionine gene. Plant Science, 164(5), 727-734.
28. Walters, D. (2000). Polyamines in plant–microbe interactions. Physiological and Molecular Plant Pathology, 57(4), 137-146.
29. Walters, D. R. (2003). Polyamines and plant disease. Phytochemistry, 64(1), 97-107.
Weydert, CJ, & Cullen, JJ. (2010), Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nature protocols, 5(1),51.
30. Xie, S.., Wu, H.-J., Zang, H.-Y., Wu, L.-M., Zhu, Q. & Gao, X.-W. (2014). Plant growth promotion by spermidine-producing Bacillus subtilis OKB105. Molecular Plant-Microbe Interactions, 27(7), 655-663.
31. Yoda, H., Fujimura, K., Takahashi, H., Munemura, I., Uchimiya, H. & Sano, H. (2009). Polyamines as a common source of hydrogen peroxide in host-and nonhost hypersensitive response during pathogen infection. Plant Molecular Biology, 70(2), 103-112.