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شناسایی سرولوژیکی و مولکولی ویروس زردی شلغم در مزارع کلزای استان کرمانشاه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

2 عضو هیأت علمی دانشگاه رازی

3 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

چکیده

اعضای جنس پولروویروس ازجمله ویروس زردی شلغم (Turnip yellows virus, TuYV) از ویروس‌های مهم در گیاهان زراعی هستند. این ویروس‌ها توسط شته‌ها به‌صورت گردشی- غیرتکثیری انتقال پیدا می‌کنند. طی سال‌های ۱۳۹۷ و ۱۳۹۸ تعداد 32 نمونه‌ی برگ کلزا و یک نمونه خردل وحشی از مزارع استان کرمانشاه جمع‌آوری شد. این نمونه‌ها با کیت تاس-الیزا DSMZ که چهار گونۀ پولروویروس شامل TuYV، Beet mild yellowing virus (BMYV)، Beet western yellows virus (BWYV) و Beet chlorosis virus (BChV) را شناسایی می‌کند، آزمایش شدند. در آزمون تاس-الیزا، هفت نمونه کلزا و نمونه خردل وحشی پاسخ مثبت نشان دادند. برای آزمون انتقال ویروس از Myzus persicae و ‌Brevicoryne brassicae استفاده شد که در گیاهان کلزا و تربچه موفقیت‌آمیز بود ولی آلودگی به چغندرقند منتقل نگردید. در IC-RT-PCR با آغازگرهای اختصاصی، یک قطعه با اندازه ۷۸۰ جفت‌باز از ژن کد کننده P0 از نمونه خردل وحشی و دو نمونه کلزا تکثیر گردید. توالی‌های نوکلئوتیدی جدایه‌های کلزا و خردل وحشی بسیار مشابه بودند (۹۹٫۴ درصد همسانی) و در جستجوی BLAST با توالی‌های نوکلئوتیدی جدایه‌های TuYV، که بسیاری از آن‌ها از استرالیا و از گیاهان مختلف مانند شنبلیله، کلزا، نخودفرنگی و نخود بودند، تا ۹۷٫۲ درصد شباهت داشتند. در تحلیل فیلوژنتیک توالی‌های نوکلئوتیدی ناحیه کدکننده P0، این دو جدایه به همراه سایر جدایه‌های TuYV در یک گروه قرار گرفته و از سایر پولروویروس‌ها مانند BMYV، BWYV و BChV متمایز بودند. در این پژوهش، شناسایی ویروس زردی شلغم در مزارع کلزای ایران بر اساس تعیین توالی نوکلئوتیدی ناحیه کدکننده P0 برای اولین بار در شهرستان کرمانشاه انجام گرفت.

کلیدواژه‌ها


عنوان مقاله [English]

Serological and molecular detection of Turnip yellows virus in rapeseed fields in Kermanshah Province, Iran

نویسندگان [English]

  • Rahele Amini 1
  • Hadi Khateri 2
  • nahid Moarrefzadeh 3
1 Former M.Sc. student, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
2 Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
3 Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
چکیده [English]

Members of the genus Polerovirus, including Turnip yellows virus (TuYV), are important viruses on field crops. They are being transmitted by aphids in a circulative non-propagative manner. During 2018 and 2019, 32 leaf samples from rapeseed (Brassica napus) and one sample from wild mustard (Sinapis arvensis) were collected in Kermanshah Province, Iran. The samples were tested by a TAS-ELISA kit (DSMZ) that detects four poleroviruses including TuYV, Beet mild yellowing virus (BMYV), Beet western yellows virus (BWYV) and Beet chlorosis virus (BChV). A positive reaction was observed for the wild mustard and seven rapeseed samples. Myzus persicae and Brevicoryne brassicae were used for virus transmission. The virus was successfully transmitted by the aphids to rapeseed and radish but not to sugar beet plants. In IC-RT-PCR using a specific primer pair, a 780 bp fragment was amplified from wild mustard and two rapeseed samples. The two nucleotide sequences from rapeseed and wild mustard had 99.4% identity to each other and showed up to 97.2% similarity to TuYV isolates previously deposited in GenBank from fenugreek, rapeseed, pea and chickpea plants in Australia. In phylogenetic analysis based on the nucleotide sequences of ORF0, two Iranian isolates of TuYV were placed in the same clade with other TuYV isolates and were distinct from other poleroviruses such as BMYV, BWYV and BChV. In this study, for the first time in Kermanshah Province, TuYV was identified in Iranian rapeseed fields based on nucleotide sequencing of the P0 coding region.

کلیدواژه‌ها [English]

  • TAS-ELISA
  • IC-RT-PCR
  • Persistent Transmission
  • rapeseed
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