c3518cb17d976b8

شناسایی سرولوژیکی و مولکولی ویروس زردی شلغم در مزارع کلزای استان کرمانشاه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

2 عضو هیأت علمی دانشگاه رازی

3 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

چکیده

اعضای جنس پولروویروس ازجمله ویروس زردی شلغم (Turnip yellows virus, TuYV) از ویروس‌های مهم در گیاهان زراعی هستند. این ویروس‌ها توسط شته‌ها به‌صورت گردشی- غیرتکثیری انتقال پیدا می‌کنند. طی سال‌های ۱۳۹۷ و ۱۳۹۸ تعداد 32 نمونه‌ی برگ کلزا و یک نمونه خردل وحشی از مزارع استان کرمانشاه جمع‌آوری شد. این نمونه‌ها با کیت تاس-الیزا DSMZ که چهار گونۀ پولروویروس شامل TuYV، Beet mild yellowing virus (BMYV)، Beet western yellows virus (BWYV) و Beet chlorosis virus (BChV) را شناسایی می‌کند، آزمایش شدند. در آزمون تاس-الیزا، هفت نمونه کلزا و نمونه خردل وحشی پاسخ مثبت نشان دادند. برای آزمون انتقال ویروس از Myzus persicae و ‌Brevicoryne brassicae استفاده شد که در گیاهان کلزا و تربچه موفقیت‌آمیز بود ولی آلودگی به چغندرقند منتقل نگردید. در IC-RT-PCR با آغازگرهای اختصاصی، یک قطعه با اندازه ۷۸۰ جفت‌باز از ژن کد کننده P0 از نمونه خردل وحشی و دو نمونه کلزا تکثیر گردید. توالی‌های نوکلئوتیدی جدایه‌های کلزا و خردل وحشی بسیار مشابه بودند (۹۹٫۴ درصد همسانی) و در جستجوی BLAST با توالی‌های نوکلئوتیدی جدایه‌های TuYV، که بسیاری از آن‌ها از استرالیا و از گیاهان مختلف مانند شنبلیله، کلزا، نخودفرنگی و نخود بودند، تا ۹۷٫۲ درصد شباهت داشتند. در تحلیل فیلوژنتیک توالی‌های نوکلئوتیدی ناحیه کدکننده P0، این دو جدایه به همراه سایر جدایه‌های TuYV در یک گروه قرار گرفته و از سایر پولروویروس‌ها مانند BMYV، BWYV و BChV متمایز بودند. در این پژوهش، شناسایی ویروس زردی شلغم در مزارع کلزای ایران بر اساس تعیین توالی نوکلئوتیدی ناحیه کدکننده P0 برای اولین بار در شهرستان کرمانشاه انجام گرفت.

کلیدواژه‌ها

عنوان مقاله [English]

Serological and molecular detection of Turnip yellows virus in rapeseed fields in Kermanshah Province, Iran

نویسندگان [English]

  • Rahele Amini 1
  • Hadi Khateri 2
  • nahid Moarrefzadeh 3
1 Former M.Sc. student, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
2 Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
3 Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran
چکیده [English]

Members of the genus Polerovirus, including Turnip yellows virus (TuYV), are important viruses on field crops. They are being transmitted by aphids in a circulative non-propagative manner. During 2018 and 2019, 32 leaf samples from rapeseed (Brassica napus) and one sample from wild mustard (Sinapis arvensis) were collected in Kermanshah Province, Iran. The samples were tested by a TAS-ELISA kit (DSMZ) that detects four poleroviruses including TuYV, Beet mild yellowing virus (BMYV), Beet western yellows virus (BWYV) and Beet chlorosis virus (BChV). A positive reaction was observed for the wild mustard and seven rapeseed samples. Myzus persicae and Brevicoryne brassicae were used for virus transmission. The virus was successfully transmitted by the aphids to rapeseed and radish but not to sugar beet plants. In IC-RT-PCR using a specific primer pair, a 780 bp fragment was amplified from wild mustard and two rapeseed samples. The two nucleotide sequences from rapeseed and wild mustard had 99.4% identity to each other and showed up to 97.2% similarity to TuYV isolates previously deposited in GenBank from fenugreek, rapeseed, pea and chickpea plants in Australia. In phylogenetic analysis based on the nucleotide sequences of ORF0, two Iranian isolates of TuYV were placed in the same clade with other TuYV isolates and were distinct from other poleroviruses such as BMYV, BWYV and BChV. In this study, for the first time in Kermanshah Province, TuYV was identified in Iranian rapeseed fields based on nucleotide sequencing of the P0 coding region.

کلیدواژه‌ها [English]

  • TAS-ELISA
  • IC-RT-PCR
  • Persistent Transmission
  • Rapeseed

مراجع

  1. Abraham, A. D., Menzel, W., Lesemann, D. E., Varrelmann, M., & Vetten, H. J. (2006). Chickpea chlorotic stunt virus: A New Polerovirus Infecting Cool-Season Food Legumes in Ethiopia. Phytopathology, 96(5), 437-446.
  2. Aftab, M., Nancarrow, N., Freeman, A., Davidson, J., Rodoni, B., & Trębicki, P. (2018). Natural infection of Cucumber mosaic virus, Pea seed-borne mosaic virus and Turnip yellows virus in a fenugreek crop (Trigonella foenum-graecum). Australasian Plant Disease Notes, 13(2), 1-6.
  3. Ahmadi, K., Ebadzadeh, H. R., Hatami, F., Abdeshah, H., & Kazemian, A. (2019). Agricultural Statistics of 2017-2018, Vol 1: Field Crops (in Persian). Tehran, Iran: Ministry of Agriculture, Statistics and Information Technology Office.
  4. (2011). Agricultural Statistics of 2010-2011, Vol 1: Field Crops (in Persian). Tehran, Iran: Ministry of Agriculture, Statistics and Information Technology Office.
  5. Asare‐Bediako, E., Jones, J. E., Hambidge, A. J., Stevens, M., Mead, A., Jenner, C. E., & Walsh, J. A. (2020). The incidence of turnip yellows virus in oilseed rape crops (Brassica napus) in three different regions of England over three consecutive growing seasons and the relationship with the abundance of flying Myzus persicae. Annals of Applied Biology, 176(2), 130-137.
  6. (2019). Myzus persicae (green peach aphid). In Invasive Species Compendium. Wallingford, UK: CAB International. www.cabi.org/isc.
  7. Coleman, A. (2013). Control of Turnip yellows virus: Assessing impact on oilseed rape quality traits and dissecting circulative transmission by aphids. (PhD Thesis), University of East Anglia, Retrieved from https://ueaeprints.uea.ac.uk/47976/
  8. Coutts, B. A., Hawkes, J. R., & Jones, R. A. C. (2006). Occurrence of Beet western yellows virus and its aphid vectors in over-summering broad-leafed weeds and volunteer crop plants in the grainbelt region of south-western Australia. Australian Journal of Agricultural Research, 57(9), 975-982.
  9. D'Arcy, C., Torrance, L., & Martin, R. (1989). Discrimination among luteoviruses and their strains by monoclonal antibodies and identification of common epitopes. Phytopathology, 79(8), 869-873.
  10. Duffus, J. E. (1975). Serological relationship between beet western yellows and beet mild yellowing viruses. Phytopathology, 65(7), 811.
  11. Duffus, J. E., & Russell, G. E. (1972). Serological relationship between Beet western yellows and Turnip yellows viruses. Phytopathology, 62(11), 1274-1277.
  12. Farzadfar, S., & Pourrahim, R. (2010). Characterizations of Iranian isolates of beet mild yellowing virus. In (pp. 33): Iranian Research Institute of Plant Protection, Agricultural Research and Education Organization.
  13. Farzadfar, S., & Pourrahim, R. (2017). Molecular detection of Turnip yellows virus (TuYV) infecting alfalfa in Iran. Australasian Plant Disease Notes, 12(1), 12.
  14. Farzadfar, S., Pourrahim, R., Golnaraghi, A. R., & Ahoonmanesh, A. (2006). Distribution and incidence of some aphid and leafhopper transmitted viruses infecting sugar beets in Iran. Plant Disease, 90(3), 252-258.
  15. Farzadfar, S., Pourrahim, R., & Maleki, M. (2014). Incidence of Beet western yellows virus (BWYV) in alfalfa fields of Lorestan and Ilam Provinces (West Iran) and its taxonomical place using coat protein gene. Applied Entomology and Phytopathology, 82(1), 37-52.
  16. Gaafar, Y. Z. A., & Ziebell, H. (2019). Two divergent isolates of Turnip yellows virus from pea and rapeseed and first report of Turnip yellows virus-associated RNA in Germany. Microbiology Resource Announcements, 8(17), e00214-00219.
  17. Graichen, K., & Rabenstein, F. F. (1996). European isolates of beet western yellows virus (BWYV) from oilseed rape (Brassica napus ssp. napus) are non-pathogenic on sugar beet (Beta vulgaris L. var. altissima) but represent isolates of turnip yellows virus (TuYV). Journal of Plant Diseases and Protection, 103, 233-245.
  18. Graichen, K., & Schliephake, E. (1999). Infestation of winter oilseed rape by turnip yellows Luteovirus and its effect on yield in Germany. Paper presented at the 10th International Rapeseed Congress - New horizons for an old crop.
  19. Hackenberg, D., Asare-Bediako, E., Baker, A., Walley, P., Jenner, C., Greer, S., Bramham, L., Batley, J., Edwards, D., Delourme, R., Barker, G., Teakle, G., & Walsh, J. (2020). Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus. Theoretical and Applied Genetics, 133(2), 383-393.
  20. Hajiusef, T., Shahraeen, N., & Maleki, M. (2016). Serological and molecular diagnosis of some viral diseases infecting chickpea (Cicer arietinum) in Lorestan and Hamadan provinces in Iran. Journal of Crop Ecophysiology, 272-280.
  21. Hauser, S., Stevens, M., Mougel, C., Smith, H. G., Fritsch, C., Herrbach, E., & Lemaire, O. (2000a). Biological, serological, and molecular variability suggest three distinct polerovirus species infecting beet or rape. Phytopathology, 90(5), 460-466.
  22. Hauser, S., Weber, C., Vetter, G., Stevens, M., Beuve, M., & Lemaire, O. (2000b). Improved detection and differentiation of poleroviruses infecting beet or rape by multiplex RT-PCR. Journal of Virological Methods, 89(1-2), 11-21.
  23. Herrbach E, Lemaire O, Ziegler-Graff V, Lot H, Rabenstein F, & Bouchery Y. (1991). Detection of BMYV and BWYV isolates using monoclonal antibodies and radioactive RNA probes, and relationships among luteoviruses. Annals of Applied Biology, 118(1), 127-138.
  24. Katul, L. (1992). Serological and molecular characterization of bean leaf roll virus and faba bean necrotic yellows virus. (PhD), University of Göttingen, Göttingen, Germany.
  25. Kehoe, M. A., & Coutts, B. A. (2019). Turnip yellows virus and Soybean dwarf virus in Western Australia. Australasian Plant Pathology, 48(4), 323-329.
  26. King, A. M. Q., Adams, M. J., Carstens, E. B., & Lefkowitz, E. J. (2012). Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses: Elsevier.
  27. Lemaire, O., Herrbach, E., Stevens, M., Bouchery, Y., & Smith, H. G. (1995). Detection of sugar beet-infecting beet mild yellowing luteovirus isolates with a specific RNA probe. The American Phytopathological Society, 85, 1513-1518.
  28. Lemmetty, A., Latvala, S., Jones, A. T., Susi, P., McGavin, W. J., & Lehto, K. (1997). Purification and properties of a new virus from black currant, its affinities with nepoviruses, and its close association with black currant reversion disease. Phytopathology, 87(4), 404-413.
  29. Makkouk, K., Kumari, S. G., Shahraeen, N., & Farzadfar, S. (2003). Identification and seasonal variation of viral diseases of chickpea and lentil in Iran. Plant Diseases and Protection, 110, 157-169.
  30. Mayo, M. A. (2002). ICTV at the Paris ICV: results of the plenary session and the binomial ballot. Archives of Virology, 147, 2254-2260.
  31. Newbert, M. J. (2016). The genetic diversity of Turnip yellows virus in oilseed rape (Brassica napus) in Europe, pathogenic determinants, new sources of resistance and host range. (PhD), School of Life Sciences, University of Warwick, UK, Retrieved from http://wrap.warwick.ac.uk/79104
  32. Schliephake, E., Graichen, K., & Rabenstein, F. (2000). Investigations on the vector transmission of the Beet mild yellowing virus (BMYV) and the Turnip yellows virus (TuYV). [Untersuchungen zur Vektorübertragung des Milden Rübenvergilbungsvirus (Beet mild yellowing virus) und des Wasserrübenvergilbungsvirus (Turnip yellows virus)]. Journal of Plant Diseases and Protection, 107(1), 81-87.
  33. Schubert, J., Rabenstein, F., Graichen, K., & Richter, K. (1998). Comparison of the 5'‐end nucleotide sequences of luteoviruses from oilseed rape and sugar beet. Archives of Phytopathology and Plant Protection, 31(6), 519-530.
  34. Shahraeen, N. (2012). An overview of oilseed rape (canola) virus diseases in Iran. International Research Journal of Microbiology, 3(1), 024-028.
  35. Shahraeen, N., Farzadfar, S., & Lesemann, D. E. (2003). Incidence of viruses infecting winter oilseed rape (Brassica napus oleifera) in Iran. Journal of Phytopathology, 151(11-12), 614-616.
  36. Smith, H., I, B., G, B., M, S., & PB, H. (1996). Production and evaluation of monoclonal antibodies for the detection of beet mild yellowing luteovirus and related strains. European Journal of Plant Pathology, 102(2), 163-169.
  37. Smith, I. M. (1988). European Handbook of Plant Diseases. London, Great Britain: Blackwell Scientific Publications.
  38. Stevens, M., McGrann, G., & Clark, B. (2008). Turnip yellows virus (syn Beet western yellows virus): an emerging threat to European oilseed rape production? HGCA Research Review No. 69: Agriculture and Horticulture Development Board, Broom’s Barn Research Centre, Higham.
  39. Stevens, M., Patron, N. J., Dolby, C. A., Weekes, R., Hallsworth, P. B., Lemaire, O., & Smith, H. G. (2005). Distribution and properties of geographically distinct isolates of sugar beet yellowing viruses. Plant Pathology, 54(2), 100-107.
  40. Wetzel, V., Brault, V., & Varrelmann, M. (2018). Production of a Beet chlorosis virus full-length cDNA clone by means of Gibson assembly and analysis of biological properties. Journal of General Virology.
  41. Wilson, C. R., Lambert, S. J., Dann, A. L., Cross, P., & Hay, F. S. (2012). Occurrence of viruses within Tasmanian vegetable crops and identification of a novel Polerovirus infecting pea. Australasian Plant Pathology, 41(3), 311-319.
  42. Zahedi Tabarestani, A., Shams-Bakhsh, M., & Safaie, N. (2012). Comparison of the coat protein gene sequence of Iranian canola- infecting Beet western yellows virus Journal of Crop Protection, 1(3), 211-219.
  43. Zahedi Tabarestani, A., Shamsbakhsh, M., & Safaei, N. (2011). Distribution of three important aphid borne canola viruses in Golestan province. Plant Protection Science, 41, 251-259.
  44. Zhou, C. J., Xiang, H. Y., Zhuo, T., Li, D. W., Yu, J. L., & Han, C. G. (2011). A novel strain of Beet western yellows virus infecting sugar beet with two distinct genotypes differing in the 5'-terminal half of genome. Virus Genes, 42(1), 141-149.
دانش گیاهپزشکی ایران
دوره 52، شماره 1
شهریور 1400
صفحه 25-36

فایل ها

سابقه مقاله

  • تاریخ دریافت: 16 مهر 1399
  • تاریخ بازنگری: 19 آبان 1399
  • تاریخ پذیرش: 27 آبان 1399

هم رسانی

ارجاع به این مقاله

آمار