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بهبود تعلیق‌پذیری، ماندگاری و زهرآگینی فرآورده‌های تجاری باکتری Bacillus thuringiensis

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه گیاه پزشکی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

هدف از این مطالعه، تعیین اثر برخی مواد افزودنی در بهبود ماندگاری و تعلیق‌پذیری فرمولاسیون پودر وتابل باکتری Bacillus thuringiensis (Bt) تهیه شده در آزمایشگاه و فرآورده‌های تجاری (A و B) بود. برای تهیه پودر وتابل بهینه از پودر خشک شده سوسپانسیون باکتری به همراه ساکارز، لاکتوز، آلجینات سدیم، فیبر نارگیل، سیلیکات آلومینیوم، در دو تیمار استفاده شد، که مبنای تفاوت دو تیمار سیلیکات آلومینیوم (فرمولاسیون بهینه شده 1) و فیبر نارگیل (فرمولاسیون بهینه شده2) قرار گرفت. حشره‌کشی پودر وتابل بهینه، روی لاروهای شب‌پره پشت الماسی، Plutella xylostella (L.) (Lep.: Plutellidae) مورد آزمون قرار گرفت. نتایج حاصل از بررسی مقایسه‌ای ماندگاری، نشان‌دهنده تأثیر مثبت این مواد در حفظ انبارداری فرمولاسیون بود. میزان تعلیق‌پذیری فرمولاسیون تهیه شده، 70 درصد ثبت شد که در مقایسه با تعلیق-پذیری فرمولاسیون‌های تجاری A و B موجود در بازار، بطور معنی‌داری بالاتر بود. نتایج نشان داد که افزودن ساکارز در هر دو فرآورده موجب افزایش زهرآگینی (کاهش مقادیر (LC50 شد در حالی که فاکتور تعلیق-پذیری، بسته به نوع و ماهیت فرآورده متفاوت عمل کرد. در نهایت افزودن مواد همراه انتخابی ارزان قیمت و در دسترس، موجب بهبود ویژگی‌های فیزیکی و زیستی فرمولاسیون‌های باکتری Bt شد که این امر در توسعه استفاده از این آفت‌کش‌ میکروبی تأثیر بسزایی دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Optimizing suspensibility, stability and virulence of commercial products of Bacillus thuringiensis

نویسندگان [English]

  • Zahra Ashjaei
  • Reza Talaei-Hassanloui
  • Ayda Khorramnejad
  • Khalil Talebi Jahromi
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The purpose of this study was to determine the effect of some additives on stability and suspensibility of wettable powder (WP) formulation of Bacillus thuringiensis (Bt) in laboratory products (optimized formulation) and commercial formulations. For preparation of optimized formulation; sucrose, lactose, sodium alginate, coconut fiber and aluminum silicate were added to the dried powder of bacterial product in two different treatments; which were different in aluminum silicate (optimized formulation 1) and coconut fiber (optimized formulation 2). The efficacy and toxicity of wettable powders were tested on diamondback moth, Plutella xylostella (L.) (Lep.: Plutellidae) larvae. The results of various tests for stability showed that the additives had positive effects on the storage life of the optimized formulation. The suspensibility of the optimized formulations (70%) was significantly higher than those of the commercial formulations. Our results showed that adding sucrose in both commercial products caused an increase in toxicity (lower LC50s), whereas, the suspensibility parameter varied depending on the type and feature of the products. In conclusion, selecting low-cost and available additives would improve the physical and biological characteristics of Bt formulations, which has a significant effect on the development and application of biopesticides.

کلیدواژه‌ها [English]

  • Wettable powder
  • Optimization
  • Sucrose
  • Formulation
  • Plutella xylostella
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